大鼠肝硬化形成过程中肝组织TGF-β1, Smad3, Smad7水平动态的变化
肝硬化;转移生长因子-β1;Smad3;Smad7;逆转录聚合酶链式反应;免疫印迹,大鼠肝硬化形成过程中肝组织TGF-β1,Smad3,Smad7水平动态的变化,俞蕾敏,吕宾,李善高,斯淑英,俞蕾敏,通讯作者:,Expressionoftransforminggrowthfactor-b1
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俞蕾敏, 吕宾, 李善高, 斯淑英, 浙江中医药大学附属第一医院消化科 浙江省杭州市 310006
俞蕾敏, 浙江中医药大学中西医结合临床消化专业硕士.
浙江省中医药管理局资助项目, No. 2005C147
通讯作者: 吕宾, 310006, 浙江省杭州市, 浙江中医药大学附属第一医院消化科. lvbin@medmail.com.cn
电话: 00571-87032028
收稿日期: 2007-07-12 修回日期: 2007-10-06
Expression of transforming growth factor-b1, Smad3 and Smad7 in experimental liver cirrhosis in rats
Lei-Min Yu, Bin Lv, Shan-Gao Li, Shu Ying Si
Lei-Min Yu, Bin Lv, Shan-Gao Li, Shu Ying Si, Department of Gastroenterology, the First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310006, Zhejiang Province, China
Supported by: Zhejiang Management Board of Traditional Chinese Medicine, No. 2005C147
Correspondence to: Bin Lv, Department of Gastroente-rology, the First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310006, Zhejiang Province, China. lvbin@medmail.com.cn
Received: 2007-07-12 Revised: 2007-10-06
Abstract
AIM: To observe changes in transforming growth factor (TGF)-b1, Smad3 and Smad7 during liver fibrosis, and to study their role hepatic tissue during the formation of experimental liver cirrhosis in rats.
METHODS: Male rats were divided into control group and model group. Control: Six male rats were killed at the beginning of the experiment as the week 0 group. Model: The surplus rats was injected subcutaneously 600 mL/L CCl4 in oil two times per week at the dosage of 3 mL/kg body weight. The rats were sacrificed after 3, 6, 9 and 12 wk. Expression of mRNA for TGF-β1 and TGF-β receptor Ⅱ (TGF-β RⅡ) in rat liver was detected by reverse transcription-polymerase chain reaction (RT-PCR), and Smad3 and Smad7 were determined by Western blotting. Meanwhile, the level of TGF-β1 was measured by immunocytochemistry.
RESULTS: TGF-β1 mRNA, TGF-β RⅡ mRNA and Smad3 at 3, 6, 9 and 12 wk were expressed in low amounts in the normal liver tissue, and were increased after CCl4 treatment, while Smad7 at the same point was decreased (TGF-β1, 0 ......
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