小鼠空肠Cajal间质细胞的分离、培养和亚甲蓝活染选择性标记
空肠;Cajal间质细胞;分离培养;亚甲蓝,祝炼,余保平,吴志轩,夏虹,通讯作者:,CultureofinterstitialcellsofCajalfromneonatalmousejejunumandselectivelivest
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祝炼, 湖北省孝感市妇幼保健院内儿科 湖北省孝感市 432000
余保平, 吴志轩, 夏虹, 武汉大学人民医院消化内科 湖北省武汉市 430060
通讯作者: 余保平, 430060, 湖北省武汉市, 武汉大学人民医院消化内科. yubaoping62@yahoo.com.cn
电话: 027-68759391
收稿日期: 2007-08-27 修回日期: 2007-10-29
Culture of interstitial cells of Cajal from neonatal mouse jejunum and selective live staining with methylene blue
Lian Zhu, Bao-Ping Yu, Zhi-Xuan Wu, Hong Xia
Lian Zhu, Department of Pediatrics, Xiaogan Maternal and Child Health Hospital, Xiaogan 432000, Hubei Province, China
Bao-Ping Yu, Zhi-Xuan Wu, Hong Xia, Department of Gastroenterology, Renmin Hospital, Wuhan University, Wuhan 430060, Hubei Province, China
Correspondence to: Dr. Bao-Ping Yu, Department of Gastroenterology, Renmin Hospital, Wuhan University, Wuhan 430060, Hubei Province, China. yubaoping62@yahoo.com.cn
Received: 2007-08-27 Revised: 2007-10-29
Abstract
AIM: To obtain intestinal pacemaker cells through dissection of the myenteric plexus of mouse jejunum, and to identify interstitial cells of Cajal (ICC) AP through selective staining with methylene blue for further electrophysiological study.
METHODS: Jejunum was removed from newborn CD-1 mice. The longitudinal muscle layer containing the myenteric plexus was cut into small pieces for culture. Live cells were stained with methylene blue and c-kit antibody.
RESULTS: Cells grown from explant culture and characterized by phenotypic features were identified as pacemaker cells, i.e. ICC-AP. Cells stained with methylene blue were also positive for c-kit antibody, whereas live neurons, smooth muscle cells and other types of ICC were not stained with methylene blue.
CONCLUSION: Pacemaker cells, i.e. ICC-AP, can be obtained through selective dissection. Methylene blue can be used for live staining identification of ICC-AP and, therefore, provides a model for further functional study.
Key Words: Jejunum; Interstitial cells of Cajal; Explant culture; Methylene blue ......
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