PPARγ表达上调体外抑制肝星状细胞的增殖
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陈 瑞, 杨胜兰
过氧化物酶体增殖物激活受体γ;肝星状细胞;凋亡;流式细胞仪,陈瑞,杨胜兰,通讯作者:,Inhibitionofproliferationofhepaticstellatecellsbyup-regulationofperoxisomeproliferat
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陈瑞, 杨胜兰, 华中科技大学附属协和医院中医科 湖北省武汉市 430022
通讯作者: 陈瑞, 430022, 湖北省武汉市汉口解放大道, 华中科技大学附属协和医院中医科. unioncr@hotmail.com
电话: 027-85726395 传真: 0411-83631284
收稿日期: 2007-03-19 修回日期: 2007-11-15
Inhibition of proliferation of hepatic stellate cells by up-regulation of peroxisome proliferator-activated receptor γ
Rui Chen, Sheng-Lan Yang
Rui Chen, Sheng-Lan Yang, Department of Chinese Traditional Medicine, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China
Correspondence to: Rui Chen, Department of Chinese Traditional Medicine, Huazhong University of Science and Technology, Jiefang Avenue, Wuhan 430022, Hubei Province, China. unioncr@hotmail.com
Received: 2007-03-19 Revised: 2007-11-15
Abstract
AIM: To evaluate the effect of up-regulated expression of peroxisome proliferators-activated receptor (PPAR)γ on hepatic stellate cell (HSC) apoptosis and proliferation.
METHODS: The rat hepatic stellate cell line (HSC-T6) was cultured in DMEM, and treated with PPARγ agonist 1, 2, 3 μmol/L 15d-PGJ2 for 24 h. The proliferation rate for HSC-T6 cells was determined in 15d-PGJ2-treated and control groups by the MTT colorimetric assay. The cell cycle and apoptosis ratio were measured by flow cytometry.
RESULTS: The expression of PPARγ mRNA in HSC-T6 cells treated with 1, 2, 3 μmol/L 15d-PGJ2 was increased (0.513 ± 0.031, 0.697 ± 0.018, 0.674 ± 0.032 vs 0.198 ± 0.021), and the proliferation of HSC-T6 cells was significantly inhibited by 15d-PGJ2, especially at a concentration of 2 μmol/L (54.6% ± 11.1%). In addition, flow cytometry suggested that the apoptosis was significantly increased in HSC-T6 cells treated with 15d-PGJ2.
CONCLUSION: Up-regulated expression of PPARγ can inhibit proliferation and induce apoptosis of HSC cells.
Key Words: Peroxisome proliferator-activated receptor γ; Hepatic stellate cell; Apoptosis; Flow cytometry ......
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