丹酚酸B对肝纤维化大鼠TGF-β1、MMP-2和TIMP-2表达的影响
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卢明芹, 潘陈为, 李 骥, 陈永平
丹酚酸B盐;肝纤维化;转化生长因子β1;基质金属蛋白酶-2;金属蛋白酶组织抑制因子-2;放射免疫法,卢明芹,潘陈为,李骥,陈永平,通讯作者:,EffectofsalvianolicacidBonlevelsofTGF-β1,M
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卢明芹, 潘陈为, 李骥, 陈永平, 温州医学院附属第一医院 浙江省温州市 325000
通讯作者: 卢明芹, 325000, 浙江省温州市, 温州医学院附属第一医院感染内科. lmq0906@163.com
电话: 0577-88078232
收稿日期: 2007-08-31 修回日期: 2007-11-08
Effect of salvianolic acid B on levels of TGF-β1, MMP-2 and TIMP-2 in rats with liver fibrosis
Ming-Qin Lu, Chen-Wei Pan, Ji Li, Yong-Ping Chen
Ming-Qin Lu, Chen-Wei Pan, Ji Li, Yong-Ping Chen, Department of Infection Medicine, the First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, Zhejiang Province, China
Correponding to: Ming-Qin Lu, the First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, Zhejiang Province, China. lmq0906@163.com
Received: 2007-08-31 Revised: 2007-11-08
Abstract
AIM: To observe the levels of TGF-β1, MMP-2 and TIMP-2 in the livers of rats with hepatic fibrosis, and the effects of salvianolic acid B (SA-B) on it.
METHODS: An animal model of hepatic fibrosis was established by injecting DMN (dimethyl nitroxide) solution into the abdominal cavity for four continuous weeks. Levels of TGF-β1, MMP-2 and TIMP-2 in liver tissue were assessed by S-P immunohistochemical staining using polyclonal antibodies against TGF-β1, MMP-2 and TIMP-2. Pathological characteristics of liver tissue were observed by microscopy after hematoxylin-eosin and Masson staining. Levels of ALT, AST and Alb were detected using an auto-biochemical analytical tool in the laboratory test department of our hospital. Serum levels of HA, LN were detected by radioimmunoassay (RIA).
RESULTS: Compared with rats in the liver fibrosis group, the pathologic manifestations of those in the SA-B-treated group improved significantly. The levels of ALT, AST, HA and LN in the treated group were significantly lower than those in the liver fibrosis group (87.0 ± 28.7 U/L vs 190.4 ± 27.4 U/L, 85.6 ± 25.3 U/L vs 178.2 ± 15.9 U/L, 179.7 ± 32.8 mg/L vs 433 ......
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