小檗碱对肝细胞核因子6 mRNA表达及葡萄糖激酶活性的影响
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闫忠卿, 冷三华, 陆付耳, 陆小红, 董 慧, 高志强
小檗碱;肝细胞核因子;原代肝细胞;葡萄糖激酶;逆转录聚合酶链反应,小檗碱对肝细胞核因子6mRNA表达及葡萄糖激酶活性的影响,闫忠卿,冷三华,陆付耳,董慧,高志强,陆小红,通讯作者:,Effectsofberberineonexpressionofhepatocytenuclearfactor6a
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闫忠卿, 冷三华, 陆付耳, 董慧, 高志强, 华中科技大学同济医学院附属同济医院中西医结合研究所 湖北省武汉市 430030
陆小红, 湖北天茂集团制剂厂 湖北省荆门市 448000
国家自然科学基金资助项目, No. 30500685
通讯作者: 陆付耳, 430030, 华中科技大学同济医学院附属同济医院中西医结合研究所. felu@tjh.tjmu.edu.cn
电话: 027-83663237 传真: 027-83663237
收稿日期: 2007-08-31 修回日期: 2007-12-13
Effects of berberine on expression of hepatocyte nuclear factor 6 and glucokinase activity in mouse primary hepatocytes
Zhong-Qing Yan, San-Hua Leng, Fu-Er Lu, Xiao-Hong Lu, Hui Dong, Zhi-Qiang Gao
Zhong-Qing Yan, San-Hua Leng, Fu-Er Lu, Hui Dong, Zhi-Qiang Gao, Institute of Integrative Traditional and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, Hubei Province, China
Xiao-Hong Lu, Hubei Biocause Pharmaceutical Co., Ltd, Jingmen 448000, Hubei Province, China
Supported by: National Natural Science Foundation of China, No. 30500685
Correspondence to: Fu-Er Lu, Institute of Integrative Traditional and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, Hubei Province, China. felu@tjh.tjmu.edu.cn
Received: 2007-08-31 Revised: 2007-12-13
Abstract
AIM: To observe the expression of hepatocyte nuclear factor 6 (HNF6) and the activity of glucokinase (GK), the key enzyme in glucose metabolism, and to investigate the possible mechanism of berberine in treating type II diabetes.
METHODS: Mouse primary hepatocytes were isolated by an improved single two-step perfusion method. The murine hepatocytes were incubated with berberine (0, 1, 3, 10, 30 and 100 μmol/L) and 1 mmol/L metformin for 24 hours. mRNA expression for HNF6 was determined by RT-PCR and analyzed by consequent quantification, and the activity of GK was detected by an enzyme kinetics method.
RESULTS: Compared with the negative control group, 1, 3, 10 or 30 μmol/L berberine promoted the expression of HNF6 mRNA (1 ......
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