重组BCG-Sj26GST疫苗诱导小鼠sIL-2R和IFN-γ的变化
作者：李文桂 石佑恩azo, http://www.100md.com
单位：李文桂(重庆医科大学附一院传染病寄生虫病研究所，重庆 400016)；石佑恩(同济医科大学寄生虫学教研室，湖北 武汉 430030)azo, http://www.100md.com
关键词：日本血吸虫；重组BCG-Sj26GST疫苗；sIL-2R；IFN-γazo, http://www.100md.com
免疫学杂志010103 摘 要：目的 研究日本血吸虫重组BCG-Sj26GST疫苗对小鼠脾细胞sIL-2R和IFN-γ的影响。方法 第1次实验采用1×106和1×108CFU疫苗皮下免疫BALB/C鼠，免疫后8周用日本血吸虫尾蚴进行攻击感染，感染后6周剖杀小鼠，同时设有PBS对照组。第2次实验用1×106CFU疫苗皮下和静脉注射分别免疫小鼠，于免疫后0、4、8、10、14和16周各剖杀4只，分离脾脏，用Sj26或ConA刺激脾细胞，用ELISA法检测脾细胞上清液中sIL2-R和IFN-γ含量。结果 疫苗免疫，尾蚴攻击后，sIL-2R和IFN-γ水平显著升高；动态观察发现sIL-2R和IFN-γ分别于免疫后8～10周和4～8周达最高水平。结论 日本血吸虫重组BCG-Sj26GST疫苗可加强宿主Th1反应，促进Th1细胞分泌IL-2和IFN-γ，它们与免疫细胞相互作用，提高宿主抗血吸虫感染的保护力。azo, http://www.100md.com
分类号：R383.2+4；R392.11 文献标识码：Aazo, http://www.100md.com
文章编号：1000-8861(2001)01-0009-04azo, http://www.100md.com
Changes of sIL-2R and IFN-γ in mice induced by recombinant BCG-Sj26GST vaccineazo, http://www.100md.com
LI Wen-gui(Institute of Infectious and Parasitic Diseases， the First Affiliated Hospital， Chongqing Medical University， Chongqing 400016，China)azo, http://www.100md.com
SHI You-en(Department of Parasitology， Tongji Medical University， Wuhan 430030， China)
Abstract：Objective To investigate the effects of recombinant BCG-Sj26GST vaccine of schistosoma japonicum on sIL-2R and IFN-γ produced by spleen cells of mice. Methods In the first experiment， BALB/C mice were immunized subcutaneously by 1×106 and 1×108 CFU vaccine respectively， challenged with Sj cercariae 8 weeks after immunization and killed 6 weeks after infection to separate spleens， PBS served as control； in the second experiment， after immunized subcutaneously and intravenously by 1×106 CFU vaccine respectively，4 mice were killed to separate spleens on 0、4、8、10、14 and 16 weeks after immunization， spleen cells were stimulated by Sj26 or ConA. Levels of sIL-2R and IFN-γ in supernatant of spleen cells were detected by ELISA.Results Levels of sIL-2R and IFN-γ increased obviously after immunization with the vaccine against challenge with Sj cercariae； dynamic observation showed sIL-2R and IFN-γ reached the highest level on 8-10 weeks and 4-8 weeks after immunization respectively. Conclusion Recombinant BCG-Sj26GST vaccine of schistosoma japonicum may strengthen host’s Th1 response， promote Th1 cells to secrete IL-2 and IFN-γ. These cytokines and immune cells may influence each other to increase host’s protection against schistosomiasis.
Keywords：schistosoma japonicum； recombinant BCG-Sj26GST vaccine； sIL-2R； IFN-γ}h0, http://www.100md.com
基金项目：国务院总理基金资助项目(94-Y-19)}h0, http://www.100md.com
作者简介：李文桂(1967-)，男，湖北郧县人，主治医师，博士，主要从事分子免疫学研究。 Tel：(023)69012724；E-mail：liwengui＠hotmail.com}h0, http://www.100md.com
参考文献：}h0, http://www.100md.com
[1]李文桂，石佑恩，汪燕鸣，等.血吸虫重组BCG-Sj26GST疫苗接种后保护力的观察[J].同济医科大学学报，1999，28(1)：13-16.}h0, http://www.100md.com
[2]李文桂，石佑恩.血吸虫重组BCG-Sj26GST疫苗诱导小鼠T淋巴细胞亚群变化的研究[J].中国人畜共患病杂志，2000，16(2)：56-58.}h0, http://www.100md.com
[3]Paul WE， Seder RA. Lymphocyte responses and cytokines [J].Cell，1994，76(1)：241-251.}h0, http://www.100md.com
[4]Boros DL. The role of cytokines in the formation of the schistosome egg granuloma [J].Immunobiol，1994，191(2)：441-450.}h0, http://www.100md.com
[5]Reynolds SR， Harn DA. Comparison of irradiated cercariae schistosoma vaccine models that use 15- and 50-kilorad doses： the 15-kilorad dose gives greater protection， smaller liver sizes and higher gamma interferon levels after challenge [J].Infect Immun，1992，60(1)：90-94.}h0, http://www.100md.com
[6]Keisari Y， Seger M， Lency J， et al.IL-1，TNF-α and IL-2 production by peritoneal and spleen cells from schistosoma mansoni infected mice and its potentiation by preimmunization with schistosomal antigens and immunostimulants[J].Immunobiol，1993，188(2)：446-459.
[7]Wynn TA， Oswald IP， Eltoum IA， et al. Elevated expression of Th1 cytokines and nitric oxide synthase in the lungs of vaccinated mice after challenge infection with schistosoma mansoni[J].J Immunol，1994，153(11)：5 200-5 209.jcx, 百拇医药
[8]Wilson RA，Coulson PS，Betts C，et al. Impaired immunity and altered pulmonary responses in mice with a disrupted interferon-gamma receptor gene exposed to the irradiated schistosoma mansoni vaccine[J].Immunol，1996，87(2)：275-282.jcx, 百拇医药
[9]Betts CJ，Wilson RA.Th1 cytokines mRNA expression dominates in the skin-draining lymph nodes of C57BL/6 mice following vaccination with irradiated schistosoma mansoni cercariae， but is down-regulated upon challenge infection[J].Immunol，1998，93(1)：49-54.jcx, 百拇医药
[10]周生华，刘述先，宋光承，等.日本血吸虫重组谷胱甘肽-S-转移酶诱生的保护性免疫应答特征的研究[J].中国寄生虫学与寄生虫病杂志，1999，17(2)：74-77.jcx, 百拇医药
[11]戴五星，郑 波，皇甫永穆.日本血吸虫重组BCG-Sj26GST疫苗对小鼠免疫应答的影响[J].同济医科大学学报，2000，29(1)：1-3.jcx, 百拇医药
[12]Cornaby A，Sunder SP，Peter JB，et al. Interleukin-2 production in plasma and urine，plasma interleukin-2 receptor levels and urine cytology as a means of monitoring renal allograft recipient[J].Transplant Pros，1988，20(1)：108-124.jcx, 百拇医药
[13]Stavitsky AB，Harold WW.Deficiency of interleukin-2 activity upon addition of soluble egg antigen to cultures of spleen cells from mice infected with schistosoma japonicum[J].Infect Immun，1998，56(7)：1 778-1 784.jcx, 百拇医药
[14]刘 莉，沈一平，管晓虹，等.日本血吸虫感染小鼠脾细胞IL-2及IFN-γ动态观察[J].中国寄生虫学与寄生虫病杂志，1995，13(1)：35-38.jcx, 百拇医药
[15]吕芳丽，石佑恩，李雍龙，等.紫外线减毒日本血吸虫尾蚴免疫小鼠的细胞免疫应答和细胞因子的动态变化[J].中国寄生虫学与寄生虫病杂志，1997，15(4)：238-242.jcx, 百拇医药
收稿日期：2000年6月9日jcx, 百拇医药
修稿日期：2000年10月11日jcx, 百拇医药
出版日期：2001年1月15日(作者：李文桂 石佑恩)
单位：李文桂(重庆医科大学附一院传染病寄生虫病研究所，重庆 400016)；石佑恩(同济医科大学寄生虫学教研室，湖北 武汉 430030)azo, http://www.100md.com
关键词：日本血吸虫；重组BCG-Sj26GST疫苗；sIL-2R；IFN-γazo, http://www.100md.com
免疫学杂志010103 摘 要：目的 研究日本血吸虫重组BCG-Sj26GST疫苗对小鼠脾细胞sIL-2R和IFN-γ的影响。方法 第1次实验采用1×106和1×108CFU疫苗皮下免疫BALB/C鼠，免疫后8周用日本血吸虫尾蚴进行攻击感染，感染后6周剖杀小鼠，同时设有PBS对照组。第2次实验用1×106CFU疫苗皮下和静脉注射分别免疫小鼠，于免疫后0、4、8、10、14和16周各剖杀4只，分离脾脏，用Sj26或ConA刺激脾细胞，用ELISA法检测脾细胞上清液中sIL2-R和IFN-γ含量。结果 疫苗免疫，尾蚴攻击后，sIL-2R和IFN-γ水平显著升高；动态观察发现sIL-2R和IFN-γ分别于免疫后8～10周和4～8周达最高水平。结论 日本血吸虫重组BCG-Sj26GST疫苗可加强宿主Th1反应，促进Th1细胞分泌IL-2和IFN-γ，它们与免疫细胞相互作用，提高宿主抗血吸虫感染的保护力。azo, http://www.100md.com
分类号：R383.2+4；R392.11 文献标识码：Aazo, http://www.100md.com
文章编号：1000-8861(2001)01-0009-04azo, http://www.100md.com
Changes of sIL-2R and IFN-γ in mice induced by recombinant BCG-Sj26GST vaccineazo, http://www.100md.com
LI Wen-gui(Institute of Infectious and Parasitic Diseases， the First Affiliated Hospital， Chongqing Medical University， Chongqing 400016，China)azo, http://www.100md.com
SHI You-en(Department of Parasitology， Tongji Medical University， Wuhan 430030， China)
Abstract：Objective To investigate the effects of recombinant BCG-Sj26GST vaccine of schistosoma japonicum on sIL-2R and IFN-γ produced by spleen cells of mice. Methods In the first experiment， BALB/C mice were immunized subcutaneously by 1×106 and 1×108 CFU vaccine respectively， challenged with Sj cercariae 8 weeks after immunization and killed 6 weeks after infection to separate spleens， PBS served as control； in the second experiment， after immunized subcutaneously and intravenously by 1×106 CFU vaccine respectively，4 mice were killed to separate spleens on 0、4、8、10、14 and 16 weeks after immunization， spleen cells were stimulated by Sj26 or ConA. Levels of sIL-2R and IFN-γ in supernatant of spleen cells were detected by ELISA.Results Levels of sIL-2R and IFN-γ increased obviously after immunization with the vaccine against challenge with Sj cercariae； dynamic observation showed sIL-2R and IFN-γ reached the highest level on 8-10 weeks and 4-8 weeks after immunization respectively. Conclusion Recombinant BCG-Sj26GST vaccine of schistosoma japonicum may strengthen host’s Th1 response， promote Th1 cells to secrete IL-2 and IFN-γ. These cytokines and immune cells may influence each other to increase host’s protection against schistosomiasis.
Keywords：schistosoma japonicum； recombinant BCG-Sj26GST vaccine； sIL-2R； IFN-γ}h0, http://www.100md.com
基金项目：国务院总理基金资助项目(94-Y-19)}h0, http://www.100md.com
作者简介：李文桂(1967-)，男，湖北郧县人，主治医师，博士，主要从事分子免疫学研究。 Tel：(023)69012724；E-mail：liwengui＠hotmail.com}h0, http://www.100md.com
参考文献：}h0, http://www.100md.com
[1]李文桂，石佑恩，汪燕鸣，等.血吸虫重组BCG-Sj26GST疫苗接种后保护力的观察[J].同济医科大学学报，1999，28(1)：13-16.}h0, http://www.100md.com
[2]李文桂，石佑恩.血吸虫重组BCG-Sj26GST疫苗诱导小鼠T淋巴细胞亚群变化的研究[J].中国人畜共患病杂志，2000，16(2)：56-58.}h0, http://www.100md.com
[3]Paul WE， Seder RA. Lymphocyte responses and cytokines [J].Cell，1994，76(1)：241-251.}h0, http://www.100md.com
[4]Boros DL. The role of cytokines in the formation of the schistosome egg granuloma [J].Immunobiol，1994，191(2)：441-450.}h0, http://www.100md.com
[5]Reynolds SR， Harn DA. Comparison of irradiated cercariae schistosoma vaccine models that use 15- and 50-kilorad doses： the 15-kilorad dose gives greater protection， smaller liver sizes and higher gamma interferon levels after challenge [J].Infect Immun，1992，60(1)：90-94.}h0, http://www.100md.com
[6]Keisari Y， Seger M， Lency J， et al.IL-1，TNF-α and IL-2 production by peritoneal and spleen cells from schistosoma mansoni infected mice and its potentiation by preimmunization with schistosomal antigens and immunostimulants[J].Immunobiol，1993，188(2)：446-459.
[7]Wynn TA， Oswald IP， Eltoum IA， et al. Elevated expression of Th1 cytokines and nitric oxide synthase in the lungs of vaccinated mice after challenge infection with schistosoma mansoni[J].J Immunol，1994，153(11)：5 200-5 209.jcx, 百拇医药
[8]Wilson RA，Coulson PS，Betts C，et al. Impaired immunity and altered pulmonary responses in mice with a disrupted interferon-gamma receptor gene exposed to the irradiated schistosoma mansoni vaccine[J].Immunol，1996，87(2)：275-282.jcx, 百拇医药
[9]Betts CJ，Wilson RA.Th1 cytokines mRNA expression dominates in the skin-draining lymph nodes of C57BL/6 mice following vaccination with irradiated schistosoma mansoni cercariae， but is down-regulated upon challenge infection[J].Immunol，1998，93(1)：49-54.jcx, 百拇医药
[10]周生华，刘述先，宋光承，等.日本血吸虫重组谷胱甘肽-S-转移酶诱生的保护性免疫应答特征的研究[J].中国寄生虫学与寄生虫病杂志，1999，17(2)：74-77.jcx, 百拇医药
[11]戴五星，郑 波，皇甫永穆.日本血吸虫重组BCG-Sj26GST疫苗对小鼠免疫应答的影响[J].同济医科大学学报，2000，29(1)：1-3.jcx, 百拇医药
[12]Cornaby A，Sunder SP，Peter JB，et al. Interleukin-2 production in plasma and urine，plasma interleukin-2 receptor levels and urine cytology as a means of monitoring renal allograft recipient[J].Transplant Pros，1988，20(1)：108-124.jcx, 百拇医药
[13]Stavitsky AB，Harold WW.Deficiency of interleukin-2 activity upon addition of soluble egg antigen to cultures of spleen cells from mice infected with schistosoma japonicum[J].Infect Immun，1998，56(7)：1 778-1 784.jcx, 百拇医药
[14]刘 莉，沈一平，管晓虹，等.日本血吸虫感染小鼠脾细胞IL-2及IFN-γ动态观察[J].中国寄生虫学与寄生虫病杂志，1995，13(1)：35-38.jcx, 百拇医药
[15]吕芳丽，石佑恩，李雍龙，等.紫外线减毒日本血吸虫尾蚴免疫小鼠的细胞免疫应答和细胞因子的动态变化[J].中国寄生虫学与寄生虫病杂志，1997，15(4)：238-242.jcx, 百拇医药
收稿日期：2000年6月9日jcx, 百拇医药
修稿日期：2000年10月11日jcx, 百拇医药
出版日期：2001年1月15日(作者：李文桂 石佑恩)