血清和卵泡液中抗精子抗体对体外受精—胚胎移植的影响
作者：宋兰林 全松 陈东红 阎宗合 邢福祺2r, http://www.100md.com
单位：第一军医大学南方医院妇产科，广东 广州 5105152r, http://www.100md.com
关键词：体外受精—胚胎移植；血清；卵泡液；抗精子抗体2r, http://www.100md.com
免疫学杂志010117 摘 要：目的 评价女性抗精子抗体对体外受精—胚胎移植(IVF-ET)的影响。方法 采用ELISA法测定施行IVF-ET的妇女的血清和卵泡液中抗精子抗体(AsAb)。A组：血清AsAb阳性13例；B组：血清及卵泡液AsAb均阳性29例；C组：AsAb阴性158例。分析3组受精率、卵裂率、胚胎发育情况及临床妊娠率和流产率的关系。结果 A、B组受精率、卵裂率明显低于C组(P<0.01)。移植日，A、B组Ⅰ—Ⅱ级胚胎率较C组低，IV级胚胎及2原核受精细胞增加(P<0.05)。各组妊娠率及流产率无显著差异(P>0.05)。结论 AsAb对IVF和早期胚胎发育有损害，体外培养不能完全清除AsAb对IVF-ET的影响。2r, http://www.100md.com
分类号：R321.2；R711.6 文献标识码：A2r, http://www.100md.com
文章编号：1000-8861(2001)01-0058-032r, http://www.100md.com
Effect of female antisperm antibodies in serum and follicular fluid on in vitro fertilization-embryo transfer2r, http://www.100md.com
SONG Lan-lin(Department of Gynecologic and Obstetric，Nanfang Hospital， the First Military Medical University， Guangzhou 510515，China)2r, http://www.100md.com
QUAN Song(Department of Gynecologic and Obstetric，Nanfang Hospital， the First Military Medical University， Guangzhou 510515，China)2r, http://www.100md.com
CHEN Dong-hong(Department of Gynecologic and Obstetric，Nanfang Hospital， the First Military Medical University， Guangzhou 510515，China)
YAN Zong-he(Department of Gynecologic and Obstetric，Nanfang Hospital， the First Military Medical University， Guangzhou 510515，China)xdd, 百拇医药
XING Fu-qi(Department of Gynecologic and Obstetric，Nanfang Hospital， the First Military Medical University， Guangzhou 510515，China)xdd, 百拇医药
Abstract：Objective To evaluate the effect of female antisperm antibodies (AsAb) on in vitro fertilization-embryo transfer(IVF-ET). Methods Concentration of AsAb of serum and follicular fluid in women undergoing IVF-ET was measured with ELISA. Group A：13 women with AsAb positive in serum， Group B： 29 women with AsAb positive in serum and follicular fluid， Group C： 158 women with AsAb negative. The IVF rate， cleavage rate， early embryonic development as well as clinical pregnancy and abortion rate were analyzed and compared among the three groups respectively.Results The IVF rate and cleavage rate in group A and B decreased significantly as compared with group C (P<0.01) . On the day of embryo transfer，Ⅰ—Ⅱgrade embryo rate in group A and B were significantly lower than that in group C.However，gradeⅣand 2PN embryo rates increased significantly as compared with group C (P<0.05). There were no significant difference in pregnancy and abortion rates among the three groups (P>0.05).Conclusion AsAb may exert a detrimental effect on IVF and early embryonic development， which can not be overcome completely by improving in vitro culture.
Keywords：in vitro fertilization-embryo transfer；serum；follicular fluid；antisperm antibodies$?, http://www.100md.com
作者简介：宋兰林(1962-)，女， 浙江温州市人，主管技师，大专，从事生殖和细胞遗传学研究。 Tel：(020)85141617；E-mail： gznfhpsl@public.guangzhou.gd.cn$?, http://www.100md.com
参考文献：$?, http://www.100md.com
[1]Marshburn PB， Kutten WH. The role of antisperm antibodies in infertility [J].Fertil Steril，1994，61(5)：799-811.$?, http://www.100md.com
[2]邢福祺，陈士岭，宋兰林，等.体外受精-胚胎移植治疗不孕症85例报道[J].中国实用妇科与产科杂志， 1998，14 (6)：353.$?, http://www.100md.com
[3]Liu DY， Clarke GN， Barer HWG. Inhibition of human sperm-zona pellucida and sperm-oolemma binding by antisperm antibodies[J]. Fertil Steril， 1991，55(2)：440-442.$?, http://www.100md.com
[4]Clarke GN， Lopata A， Johnston WIH. Effect of sperm antibodies in females on human in vitro fertilization [J]. Fertil Steril，1986，46(3)：435-441.$?, http://www.100md.com
[5]Vazquez-Levin M， Kaplan P，Guzman I， et al. The effect of female antisperm antibodies on in vitro fertilization， early embryonic development， and pregnancy outcome[J]. Fertil Steril， 1991，56(1)：84-88.$?, http://www.100md.com
收稿日期：2000年8月23日$?, http://www.100md.com
修稿日期：2000年12月4日$?, http://www.100md.com
出版日期：2001年1月15日(作者：宋兰林 全松 陈东红 阎宗合 邢福祺)
单位：第一军医大学南方医院妇产科，广东 广州 5105152r, http://www.100md.com
关键词：体外受精—胚胎移植；血清；卵泡液；抗精子抗体2r, http://www.100md.com
免疫学杂志010117 摘 要：目的 评价女性抗精子抗体对体外受精—胚胎移植(IVF-ET)的影响。方法 采用ELISA法测定施行IVF-ET的妇女的血清和卵泡液中抗精子抗体(AsAb)。A组：血清AsAb阳性13例；B组：血清及卵泡液AsAb均阳性29例；C组：AsAb阴性158例。分析3组受精率、卵裂率、胚胎发育情况及临床妊娠率和流产率的关系。结果 A、B组受精率、卵裂率明显低于C组(P<0.01)。移植日，A、B组Ⅰ—Ⅱ级胚胎率较C组低，IV级胚胎及2原核受精细胞增加(P<0.05)。各组妊娠率及流产率无显著差异(P>0.05)。结论 AsAb对IVF和早期胚胎发育有损害，体外培养不能完全清除AsAb对IVF-ET的影响。2r, http://www.100md.com
分类号：R321.2；R711.6 文献标识码：A2r, http://www.100md.com
文章编号：1000-8861(2001)01-0058-032r, http://www.100md.com
Effect of female antisperm antibodies in serum and follicular fluid on in vitro fertilization-embryo transfer2r, http://www.100md.com
SONG Lan-lin(Department of Gynecologic and Obstetric，Nanfang Hospital， the First Military Medical University， Guangzhou 510515，China)2r, http://www.100md.com
QUAN Song(Department of Gynecologic and Obstetric，Nanfang Hospital， the First Military Medical University， Guangzhou 510515，China)2r, http://www.100md.com
CHEN Dong-hong(Department of Gynecologic and Obstetric，Nanfang Hospital， the First Military Medical University， Guangzhou 510515，China)
YAN Zong-he(Department of Gynecologic and Obstetric，Nanfang Hospital， the First Military Medical University， Guangzhou 510515，China)xdd, 百拇医药
XING Fu-qi(Department of Gynecologic and Obstetric，Nanfang Hospital， the First Military Medical University， Guangzhou 510515，China)xdd, 百拇医药
Abstract：Objective To evaluate the effect of female antisperm antibodies (AsAb) on in vitro fertilization-embryo transfer(IVF-ET). Methods Concentration of AsAb of serum and follicular fluid in women undergoing IVF-ET was measured with ELISA. Group A：13 women with AsAb positive in serum， Group B： 29 women with AsAb positive in serum and follicular fluid， Group C： 158 women with AsAb negative. The IVF rate， cleavage rate， early embryonic development as well as clinical pregnancy and abortion rate were analyzed and compared among the three groups respectively.Results The IVF rate and cleavage rate in group A and B decreased significantly as compared with group C (P<0.01) . On the day of embryo transfer，Ⅰ—Ⅱgrade embryo rate in group A and B were significantly lower than that in group C.However，gradeⅣand 2PN embryo rates increased significantly as compared with group C (P<0.05). There were no significant difference in pregnancy and abortion rates among the three groups (P>0.05).Conclusion AsAb may exert a detrimental effect on IVF and early embryonic development， which can not be overcome completely by improving in vitro culture.
Keywords：in vitro fertilization-embryo transfer；serum；follicular fluid；antisperm antibodies$?, http://www.100md.com
作者简介：宋兰林(1962-)，女， 浙江温州市人，主管技师，大专，从事生殖和细胞遗传学研究。 Tel：(020)85141617；E-mail： gznfhpsl@public.guangzhou.gd.cn$?, http://www.100md.com
参考文献：$?, http://www.100md.com
[1]Marshburn PB， Kutten WH. The role of antisperm antibodies in infertility [J].Fertil Steril，1994，61(5)：799-811.$?, http://www.100md.com
[2]邢福祺，陈士岭，宋兰林，等.体外受精-胚胎移植治疗不孕症85例报道[J].中国实用妇科与产科杂志， 1998，14 (6)：353.$?, http://www.100md.com
[3]Liu DY， Clarke GN， Barer HWG. Inhibition of human sperm-zona pellucida and sperm-oolemma binding by antisperm antibodies[J]. Fertil Steril， 1991，55(2)：440-442.$?, http://www.100md.com
[4]Clarke GN， Lopata A， Johnston WIH. Effect of sperm antibodies in females on human in vitro fertilization [J]. Fertil Steril，1986，46(3)：435-441.$?, http://www.100md.com
[5]Vazquez-Levin M， Kaplan P，Guzman I， et al. The effect of female antisperm antibodies on in vitro fertilization， early embryonic development， and pregnancy outcome[J]. Fertil Steril， 1991，56(1)：84-88.$?, http://www.100md.com
收稿日期：2000年8月23日$?, http://www.100md.com
修稿日期：2000年12月4日$?, http://www.100md.com
出版日期：2001年1月15日(作者：宋兰林 全松 陈东红 阎宗合 邢福祺)