Volume10 September2003 Number 5
CHINESE JOURNAL OF INTEGRATED TRADITIONAL
AND WESTERN MEDICINE IN INTENSIVE AND CRITICAL CARE
Volume 10 September 2003 Number 5
PAGE：259-261
Clinical effective observation on integrated traditional Chinese and western medicine in treatment of 51 severe SARS patients
LI Xiu-hui, HU Jian-hua, ZHANG Ke, YE Jun, GOU Chun-yan, LI Yan, ZHANG Bin
, 百拇医药
Beijing Youan Hospital, Beijing 100054, China.
Abstract: Objective: To evaluate the clinical effect of integrated traditional Chinese and western medicine (TCM WM) in treatment of severe SARS patients. Methods: Prognosis and T subset of 51 severe SARS patients admitted to our hospital from March 11 to May 30, 2003 were analyzed. Results: Of 29 cases, 26 cases (86.65%) were cured and 3 cases (10.34%) died in TCM WM group. However, of 22 cases, 12 cases (54.5%) were cured and 10 cases(45.5%) died in western medicine group, it had significance difference (P<0.05 ) between two groups. Clinical symptom improved significantly in TCM WM group, it had significant difference compared with western medicine group (P<0.01). CD+4 T lymphocyte count increased from (361±278)×106/L to (630±454)×106/L in TCM WM group (P<0.05)， and from (288±186)×106/L to (376±285)×106/L in western medicine group(P<0.05). Conclusion: Compared with western medicine treatment, TCM WM treatment can improve clinical symptom and immunity, reduce mortality of severe SARS patients.
, 百拇医药
PAGE：262-264
Dynamic observation on changes of malondialdehyde, nitric oxide and superoxide dismutase in patients with severe acute respiratory syndrome and its significance
ZHUANG Jun-hua, HUANG Xian-zhang, LIN Li, PAN Wan-yi， LIN Lin.
Clinical Laboratory, Ersha Island Branch, Guangdong Provincal Hospital of Traditional Chinese Medicine, Guangzhou 510105, Guangdong, China.
, 百拇医药
Abstract: Objective: To dynamically observe the changes of malondialdehyde (MDA), nitric oxide (NO) and superoxide dismutase (SOD) in patients with severe acute respiratory syndrome(SARS) and to explore its significance. Methods: MDA, NO and SOD were determined by using bathyran chromatometry, nitrate reductase method, xanthine oxidase inhibition method in SARS patients during the first, recovery and follow-up stage, the front health doctors and nurses and health people. The significance of their changes were analyzed. Results: The mean of MDA during the first stage was higher than that of recovery stage and the mean of recovery stage was higher than that of follow-up stage and health people (all P<0.001).The mean of NO during the first stage was higher than that of other stages. The NO means of recovery and follow-up stage were secondly higher. The mean of SOD during the first stage was higher than that of recovery stage and was lower than that of health people(P=0.024), the mean of SOD during the recovery stage was lower than that of follow-up stage, the front health doctors and nurses and health people (all P<0.001). Conclusion: The pathological injury in pathogenesis of SARS is related to free radicals. SOD has the capacity of anti-injury.
, 百拇医药
PAGE：265-267
Study on traditional Chinese medical diagnosis and treatment based on differentiation of syndromes and signs in 30 severe acute respiratory syndrome patients
XIAO He-yin, ZHAO Ji-ping, ZHOU Shao-zhong, SHEN Chun, WANG Ji-dong, CUI Hong-sheng, ZHANG Xue-xun, LIU Ying-jie, CHEN Dong-mei, TAO Hong
Dongzhimen Hospital, Beijing University of Traditional Chinese Medicine, Beijing 100700, China.
, 百拇医药
Abstract: Objective: To investigate the traditional Chinese medical features of the different symptomatic types of severe acute respiratory syndrome (SARS) and assess the effects of integrated traditional Chinese and western medicine. Methods: According to the different symptomatic types of 30 SARS patients in Dongzhimen Hospital from April 19 to May 1, 2003, they were treated by traditional Chinese medicine herbs based on general treatment including steroid, anti-virus medicine, mechanical ventilation, symptomatic messure and so on. Results: At initial stage 30 cases all had Qi-fen(气分) syndrome such as high fever. They seldom had light fever as Wei-fen(卫分) syndrome. Four cases of them suffered diarrea. As traditional Chinese medicine theory SARS patients had three stages: heat-evil at Qi-fen, flaming of heat-evil at Qi-fen and Ying-fen(营分), heat-evil at Xue-fen(血分). Patients of Qi-fen type were treated with clearing away the heat-evil at Qi-fen, releasing inhabited lung-energy and dissipating phlegm, clearing away heat and toxic materials. The Chinese medicine prescriptions were Wuhu decoction(五虎汤) and Tingli Dazao Xiefei decoction(葶苈大枣泻肺汤). Patients of Qi-Ying-fen(气营分) type were treated with clearing away the heat-evil and phlegm located at Qi-Ying-fen. The Chinese medicine prescriptions were Qingwen Baidu drink(清瘟败毒饮) and Mengshi Guntan decoction(礞石滚痰汤). Patients of Xue-fen type were treated with cooling blood and clearing away the heat-evil, phlegm in the pericardium and waking up the patients from unconsciousness. The Chinese medicine prescriptions were Xijiao Dihuang decoction(犀角地黄汤) and Taohong Siwu decoction (桃红四物汤). Salvia miltiorrhiza injection(丹参注射液) were used in Ying-Xue-fen syndrome. Shengmai injection(生脉注射液) were used in shortage of energy and blood. They were very effective. Up to May 1 among 30 SARS patients, 3 cases recoverd and discharged, 4 cases were dead, the others were transferred to the special hospital, they felt much better. Severe patients had weaning from mechanical ventilation, and the changes in clest X-ray radiogram were improved. Conclusion: According to traditional Chinese medicine theory, SARS belongs to seasonal febrile disease. Its development follows Wei, Qi, Ying, Xue (卫气营血) regulation. The symptom of SARS patients can be obviously improved by the treatment of integrated traditional Chinese and western medicine. The method of activating blood circulation to dissipate blood stasis can be used at every stage.
, 百拇医药
PAGE：268-270
Symptoms of 126 SARS patients in recovering period
CHEN Jing, LIU Wei， YUAN Hui-qing, YU Zhen, YI Jing-hong, WANG Xu-hong, LIU Hui-lin, XUAN Ya-bo, ZHANG Jie, MENG Guang-song
Beijing Hospital of Traditional Chinese Medicine, Beijing 100010, China.
Abstract: Objective: To gather the information of SARS patients who were out of hospital and find their symptoms in order to provide the reasons to treat SARS in recovery period. Methods: The patients of the community in Beijing were inspected, their symptoms, tongue and pulse condition, laboratorial test of blood, result of X-ray and CT were gathered and sumed up. Results: A total of 126 cases were researched, 89 patients were inspected at all. Breathe hard, easy tired, sweat more, dry mouth or bitter taste, feverish sensation, tongue body red, coating thick and greasy, pulse slippery thin and rapid were appeared in more than 47% patients. In about 10 percent-20 percent patients, the results of laboratorial test of blood were abnormal. In 10 percent patients, electrocardiogram were abnormal. Fifty-five cases were found remained shadow in X-ray film. Conclusion: It is necessary to be treated and observed in recovering period in the patients with SARS.
, 百拇医药
PAGE：271-274
Analysis on treatment of severe acute respiratory syndrome by integrated traditional Chinese and western medicine
HE Xiao-yun, HOU Yi-jun, ZHANG Yan-lei, ZHOU Yun-zhi， ZHANG Tao， YUAN Ying, LI Hong-mei
Department of Respiration, The Coal General Hospital, Beijing 100028, China.
Abstract: Objective: To analyze the treatment of serious cases with severe acute respiratory syndrome (SARS) by integrated traditional Chinese and western medicine. Methods: Effect of integrated traditional Chinese and western medicine treatment was reviewed retrospectively on 91 patients of SARS hospitalized in the Coal General Hospital from April 30 to June 5，2003. Results: The mean dosage of glucocorticoid was 153.33 mg/d in treatment group and 246.00 mg/d in control group. The time treating by glucocorticoid was 13.00 days in treatment group and 15.53 days in control group. In patients treated with traditional Chinese and western medicine, the chest X-ray radiogram showed lung fibrosis was ameliorated. It indicated that integrated traditional Chinese and western medicine treatment could reduce the dosage of steroid hormone for serious cases of SARS and shorten time of using steroid hormone in vein. It produced also some effects to prevent SARS patients' condition from worsening and improve the prognosis of serious cases of SARS. Conclusion: Active integrated traditional Chinese and western medicine treatment in the early stage is better in improving the condition, shortening the disease course and reducing the pulmonary fibrosis for SARS patients.
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PAGE：275-278
Effect of naloxone on spinal cord ischemic tolerance induced by pretreatment with repeated electroacupuncture
LEI Yi, XIONG Li-ze, CHEN Shao-yang，HU Bo, ZENG Yi, WANG Qiang, HE Da-yin
Department of Anesthesiology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, Shanxi, China
Abstract: Objective: To study the effect of opioid on spinal cord ischemic tolerance induced by pretreatment with repeated electroacupuncture(EA) in rabbits. Methods: Forty New Zealand white rabbits were randomly divided into five groups (n=8): animals in the control group received no treatment; animals in the sodium pentobarbital(SP) group received SP 30 mg/kg intravenously each day for 5 days; animals in naloxone group received naloxone 0.3 mg/kg intravenously each day for 5 days; animals in EA group were anesthetized with SP 30 mg/kg intravenously and received electroacupuncture either at the Weizhong acupoint(委中穴) 60 minutes each day for 5 days; animals in EA and naloxone group were injected naloxone 0.3 mg/kg intravenously 30 minutes before pretreatment and were anesthetized with SP 30 mg/kg intravenously and received electroacupuncture either at the Weizhong acupoint 60 minutes each day for 5 days. Twenty-four hours after last treatment, spinal cord ischemia was induced by an infrarenal aortic occlusion for 20 minutes. Hind-limb motor function was determined with the Tarlov criteria (in which 4 is normal and 0 is paraplegia) at 4, 8, 12, 24, and 48 hours after reperfusion. All animals were sacrificed at 48 hours after reperfusion and the spinal cords (L5-L7) were removed immediately for histopathologic study. Results: All the animals survived the experiment. The neurologic and histopathologic outcomes in the EA group 48 hours after reperfusion were significantly better than the EA and naloxone group (all P<0.01), but no differences were found among control group, SP group, naloxone group and EA and naloxone group (all P>0.05).Conclusion:The effect of preconditioning with repeated electroacupuncture induces neuroprotection against spinal cord ischemic-reperfusion injury can be restrained by naloxone, which imply opioid relates with the mechanism of repeated electroacupuncture against spinal cord ischemic-preconditioning.
, 百拇医药
PAGE：279-281
Experiment study of salvia miltiorrhiza on inhibiting polymorphonuclear leukocyte and endothelial cells adhesion in acute pancreatitis rabbits
SHA Jian-ping， ZHU Bi-de， XU Yan， CHEN Xuan， ZHAO Yan， LIU Xiao， LU Dian-rong， LIU Zhou
Chengdu University of Tradtional Chinese Medicine, Chengdu 610072, Sichuan, China.
Abstract: Objective: To study the mechanism of polymorphonuclear leukocyte(PMN) and endothelial cell(EC) adhesion in the early period of acute pancreatitis(AP) and the effect of salvia miltiorrhiza on AP. Methods: Rabbit AP model was established. Twenty-four Japan rabbits were divided randomly into normal saline group which was treated with normal saline and the salvia miltiorrhiza treatment group which was treated with saliva miltiorrhiza. Blood was obtained at pro-operation, post-operation instantly, 2 hours, 4 hours, 8 hours, 12 hours, 16 hours and 24 hours after operation. PMN was purred out of the blood, and interacted with rabbit's vascular EC which was cultured out of the body, the adhesion ratio of PMN-EC and CD11a/CD18, CD11b/CD18 expression was measured. Results: After operation, both CD11a/CD18, CD11b/CD18 expression and the PMN-EC adhesion ratio were increased immediately, the normal saline group reached the peak at 4 hours after the operation, the salvia miltiorrhiza treatment group was dramatically lower compared with those of the normal saline group at 2 hours, 4 hours, 8 hours, 12 hours, 16 hours and 24 hours after operation. Conclusion: Salvia miltiorrhiza can lower the CD11a/CD18, CD11b/CD18 expression, inhibit PMN-EC adhesion, improve the microcirculation, ease the tissue damage produced by PMN-EC adhesion and help the AP treatment in the early period.
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PAGE：282-284
Changes of β-endorphin in different tissue of the rabbits with incomplete trauma paraplegia by acupuncture
LIU Jie, HU Xiang-ming, LIU Guang-guo, WANG Qun, LIU Zhi, DING Zhi-gao, ZHANG Guo-min
The First Affiliated Hospital, Hunan College of Traditional Chinese Medicine, Changsha 410007, Hunan, China.
Abstract: Objective: To observe the effect of acupuncture on the change of β-endorphin(β-EP) in the rabbits with incomplete paraplegia, and to explore the biochemical effect of acupuncture to repair of damaged spinal cord. Methods: Acupuncture were applied to governor vessel and nerve trunk for treatment of the rabbits with incomplete paraplegia. The concentration of β-EP in the plasma, pituitary, injuried spinal cord and hypothalamus of the rabbits was determined at 6 hours, 7 days and 15 days. Results: The concentration of β-EP in rabbits after acupuntured once (6 hours ago) decreased significantly compared to the model group. The regulation of β-EP level by acupunture was fast in the blood plasma and pituitary of the rabbits and it descended by stage at 6 hours, 7 days and 15 days. There was a significant difference between 6 hours and 15 days about β-EP levels of hypothalmas. The β-EP level in spinal cord began to descend at 7 days. Conclusion: Acupuncture treatment can facititate the damaged spinal cord repair by degrading and diffusing β-EP, blocking β-EP release, reducing β-EP secretion immediately, thus decreasing the level of β-EP in the blood and reducing β-EP combination with the β-EP receptor of target cells,counteracting the secondary injure of β-EP to spinal cord.
, 百拇医药
PAGE：285-286
Clinical study on treatment with Xuanjiang Feiqi mixture(宣降肺气方) for acute seizure phase of chronic cor pulmonale
YANG Mao-nong, XU Fang-yuan, LIU Yue-jian, CHEN Jin, GAN Jin-hua
The People's Hospital of Sichuan Province, Chengdu 610072, Sichuan, China.
Abstract: Objective: To observe the curative efficacy of Xuanjiang Feiqi mixture(XJFQM,宣降肺气方) combined with western medicine(WM) for acute seizure phase of chronic cor pulmonale. Methods: Sixty-two cases with acute seizure phase of cor pulmonale were randomly divided into two groups. The treatment group(n=31) was treated with both conventional therapy of WM and XJFQM, and the control group(n=31) was treated with conventional WM therapy described above only. Ten days after the treatment, the level of plasma endothelin-1(ET-1) and blood gas indices were detected in both groups, and the therapeutic effects were observed. Results: The markedly effective rate of the treatment group(67.74%) was higher than that of the control group(41.94%,P<0.05). Meanwhile the improvements of plasma ET-1,partial pressure of oxygen in artery(PaO2), partial pressure of carbondioxide in artery (PaCO2) in each group after the treatment were more obvious than that before the treatment(P<0.05 or P<0.01), and the changing extend were more obviously in treatment group than that in control group (all P<0.05). Conclusion: The treatment of XJFQM combined WM for acute seizure phase of chronic cor pulmonale is able to heighten the curative efficacy significantly.
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PAGE：287-289
Effect of Jiawei Zhenwu decoction(加味真武汤) on plasma endothelium in rabbits with congestive heart failure belonged to Yang-deficiency and water-stagnation symptom(阳虚水停证) of Saoyin disease(少阴病)
LONG Xin-sheng, LU Ping, MO Jin-song, LI Fa-la, ZHANG Fei-hui, QIU Xia, WANG Ju-ping, ZHANG Zhi-ling
Shenzhen Hospital of Traditional Chinese Medicine, Shenzhen 518033, Guangdong, China.
, 百拇医药
Abstract: Objective: To study the effect of Jiawei Zhenwu decoction(加味真武汤) on plasma endothelium in rabbits with congestive heart failure (CHF) belonged to Yang-deficiency and water-stagnation symptom(阳虚水停证) of Saoyin disease(少阴病). Methods: By first infusing large dose of cold nature drugs, followed by ligaturing the anterior descending branch of coronary artery and gradually narrowing down their aortic caliber, the rabbit model of Yang-deficiency and water-stagnation symptom of Saoyin disease in CHF was successfully established. The rabbits were divided into four groups: traditional Chinese medicine group was infused Jiawei Zhenwu decoction, western medicine group was treated with captopril, traditional Chinese medicine and western medicine group was treated with Jiawei Zhenwu decoction and captopril, control group was not received medicine. The plasma endothelin was measured in each group. Results: The level of plasma endothelin in the model rabbit was significantly higher than that in normal rabbits(P<0.05). Jiawei Zhenwu decoction could lower the level of plasma endothelin in the model rabbit, there was obviously difference between traditional Chinese medicine group and control group (P<0.01). There was not obviously difference between traditional Chinese medicine group and western medicine group(P>0.05). Conclusion: The low of plasma endothelin level was one of the mechanisms of treatment function of Jiawei Zhenwu decoction on CHF.
, 百拇医药
PAGE：290-292
Clinical observation on the effect of Ganxian prescription(肝纤方) combined lamivudine in treating 31 patients with chronic hepatitis B
SHEN Wei-sheng， YANG Hong-zhi, HONG Qi, ZHANG Yong-qiang, DAI Min
Jiangyin People's Hospital, Jiangyin 214400, Jiangsu, China
Abstract: Objective: To assess the therapeutic effects of Ganxian prescription(肝纤方) combined lamivudine in treating chronic hepatitis B. Methods: Thirty-one patients with chronic hepatitis B in treatment group were treated with Ganxian prescription and lamivudine, while 37 patients in control group were just received lamivudine. The therapeutic duration in both group were one year. Results: The overall effective rate including wholly and partly response was 90.3% in treatment group and 70.3% in control group (P<0.05). No tyrosine-methionioe-aspartate-astartate (YMDD) mutants were found in treatment group but 5 in control group(16.7%). The other objective markers in treatment group were superior to those in control group. Conclusion: Ganxian prescription can offset the limitation of lamivudine. The therapeutic effects can be significantly improved by Ganxian prescription combined lamivudine to treat chronic hepatitis B.
, 百拇医药
PAGE：293-295
Clinical comparison of cerebral vascular spasm treated with Yangxue Qingnao granular(养血清脑颗粒)
ZHANG Jian-jun， DONG Wei-feng, ZHANG Jun， XUAN Hong-fei， GU Shui-jun, XIE Ren-long, SI Yun
Xiaoshan the First People's Hospital, Hangzhou 311201, Zhejiang, China.
Abstract: Objective: To observe the effects of Yangxue Qingnao granular(养血清脑颗粒) in treating cerebral vascular spasm (CVS) caused by different cause. Methods: One hundred and twenty cases with CVS approved by transcraninal Doppler ultrasonography(TCD) were divided randomly into three groups, patients in A group were treated with Yangxue Qingnao granular and basic treatment. Patients in B group were treated with nimodipine and basic treatment. Patients in C group were treated with basic treatment only. The TCD test was repeated and the data of clinical sign were observed and analyzed after 4 weeks. Results: The outcome of treatment to deal with CVS in A and B group was better than that in C group (P<0.01). The outcome of treatment to deal with CVS caused by subarachnoid hemorrhage (SAH) in A group was better than that in B group (P<0.05). Conclusion: Yangxue Qingnao granular has the obvious therapeutic effect in treatment of CVS, especially in CVS which is not caused by SAH.
, 百拇医药
PAGE：296-298
Effect of Lingxie capsule(羚蝎胶囊) on circulating endothelial cells of rats with focal cerebral ischemia/reperfusion injury
ZHU Dong-sheng, MA Zhi-heng, CHEN Yi-wei, ZHU Jun， WANG Fang, WEI Hong-chang, XU Min-hua
Shanghai Second People's Hospital, Shanghai 200011, China.
Abstract: Objective: To observe the effect of Lingxie capsule(羚蝎胶囊) on circulating endothelial cells (CEC) of rats with focal cerebral ischemia/reperfusion(I/R) injury, and to investigate the mechanism of Lingxie capsule in treatment of cerebral infarction. Methods: Thirty-six SD rats were randomly divided into three groups, the sham-operated group, the control group and the Lingxie capsule treatment group. Rat model of cerebral I/R injury was established by the middle cerebral artery occlusion. The neurofunction deficit score and CEC were observed in cerebral I/R model rats. Results: Significant difference was found between the control group and the Lingxie capsule treatment group in decrease of neurofunction deficit score 3 hours after operation 〔(2.30±0.34) scores in Lingxie capsule treatment group and (3.10±0.23) scores in model group, P<0.05〕 and the count of CEC 〔(5.57±0.89) cells/0.9 μl in Lingxie capsule treatment group and (6.38±0.91)cells/0.9 μl in model group, P<0.05〕. Conclusion: Lingxie capsule might play an important role in reducing CEC of rats with focal cerebral I/R injury, and it may be the mechanism of treating cerebral infarction.
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PAGE：299-301
Protective effects of tetramethylpyrazine in patients with acute cerebral ischemia/ reperfusion injury after cardiopulmonary resuscitation
LOU Zheng-jia, ZHUGE Li-min, ZHENG Wen-long, OUYANG Kan, SHOU Lu
Department of Emergency, Hangzhou Hospital of Traditional Chinese Medicine, Hangzhou 310007, Zhejiang, China.
Abstract: Objective: To evaluate the protective effects of tetramethylpyrazine on acute cerebral ischemia/reperfusion injury in patients after cardiopulmonary resuscitation. Methods: Forty-two cases who were going to be treated with cardiopulmonary resuscitation were randomly divided into two groups, 22 cases in treatment group (A group) and 20 cases in control group (B group). Both groups were treated with tetramethyplyrazine 240 mg added 5% glucose 250 ml infused intravenously for 1 hour, once a day for 7 days. The additional treatment of tetramethylpyrazine infusion was administered in A group while in B group was not. The changes of plasma superoxide dismutase (SOD), malondialdehyde (MDA) and thromboxane B2 (TXB2) were determined in each group before and after therapy. The conciousness recovery was as the main index to valuating the clinical curative effects. Results: The marked effective rate, effective rate and no effective rate in A group(72.72%, 13.64%, 13.64%) was significantly higher than those in B group (40.00%, 15.00%， 45.00%, all P<0.05). The plasma SOD, MDA and TXB2 levels were more remarkably improved in A group (all P<0.01). Conclusion: Treating with tetramethylpyrazine can restrain the over oxidation of lipid, bate the conglomeration of platelet and evaluate the activity of SOD，even can elevate the rate of cerebral resuscitation. It can prevent patients from acute cerebral ischemia/reperfusion injury after cardiopulmonary resuscitation.
, 百拇医药
PAGE：302-303
Clinical observation on effect of Puerarin(葛根素) combined with meglumine adenosine cyclophosphate in treatment of refractory heart failure
WANG Yong-jian, GUO Peng, QU Yu-zhen
Emergency Department,the Third People's Hospital of Jinan, Jinan 250101, Shandong, China.
Abstract: Objective: To explore the clinical effect of Puerarin combined with meglumine adenosine cyclophosphate in treatment of refractory heart failure. Methods: Sixty-two cases with refractory heart failure were randomly divided into control group (n=30) and treatment group (n=32). The control group was treated with routine therapy including oxygen inhalation, diuretic, digitalis, vasodilator, angiotensin-converting enzyme inhibitor (ACEI), antibiotic therapy, and supporting treatment. The treatment group was treated with Puerarin 0.4 g+5% glucose 250 ml intravenously drip once a day for 14 days, meglumine adenosine cyclophosphate 120 mg+5% glucose 250 ml intravenously, drip once a day for 14 days, as well as routine therapy. After two weeks, the clinical effects were compared between two groups. Results: The heart function and cardiac indexes were improved after treatment in two groups (all P<0.01), the difference was significant between treatment group and control group after treatment (P<0.05). Conclusion: Combined Puerarin with meglumine adenosine cyclophosphate in treating refractory heart failure is effective and safe.
, 百拇医药
PAGE：304-306
Myocardial injury and changes of nitric oxide content in hemorrhagic shock/ reperfusion and the effect of ganoderma lucidum polysaccharide on them
YANG Hong-mei，WANG Li， CHEN Jie， PEI Rui, XU Qiu-xia, GUO An-qi, GUI Xing-fen. Henan Medical College for Staff and Worker, Zhengzhou 450003, Henan, China.
Abstract: Objective: To investigate the myocardial injury and changes of nitric oxide (NO) content in hemorrhagic shock / reperfusion (HS-R) rabbits and the effect of ganoderma lucidum polysaccharide (GLP) on them. Methods: Using the HS-R model of rabbits, animals were divided into three groups: sham operation group, reperfusion with normal saline (NS) group, reperfusion with 1% GLP group. The mean artery pressure (MAP), ventricular function, NO content in serum and myocardium, the activity of NOS in myocardium were examined. Results: At 40 minutes after hemorrhagic shock, left ventricular end-diastolic pressure(LVEDP) were slightly increased in the two reperfusion groups, but the difference was not significant (all P>0.05). The maximum rate of intraventricular pressure rise (+dp/dtmax) and maximum rate of intraventricular pressure down (-dp/dtmax) were reduced, and NO content in serum were increased significantly compared with those before shock and in sham operation group (all P<0.05). At 40 minutes after reperfusion, ventricular functions were reduced, NO content in serum and myocardium, the activity of NOS in myocardium were all increased significantly in reperfusion with NS group compared with those in sham operation group (all P<0.05). At the same time, the cardiac function was improved, NO concentration in serum and myocardium, the activity of NOS in myocardium were significantly decreased in reperfusion with 1% GLP group than those in reperfusion with NS group (all P<0.05). Conclusion: In HS-R process, the activity of NOS, the NO content in serum and myocardium are all increased, the cardiac function is decreased. The myocardial injury in HS-R is related with changes of NO content. GLP has protective effect on myocardial injury in HS-R .Its mechanism would be inhibiting the activity of NOS and reduced NO content in myocardium.
, 百拇医药
PAGE：307-309
Effects of traditional Chinese herbs Zhixiao Pingchuan prescription(止哮平喘方) on Th1/Th2 cells in guinea-pigs asthma model
HU Zuo-wei, ZHOU Yan-ping, WANG Peng
Institute of Integrated Traditional Chinese and Western Medicine, Fudan University, Shanghai 200040, China.
Abstract: Objective: To investigate the effects of traditional Chinese herbs Zhixiao Pingchuan prescription(止哮平喘方) on Th1/Th2 cells in guinea-pigs asthma model. Methods: CD+ 4 and CD+ 8 T cells counts were detected with immunocytochemistry (ICC) in the peripheral blood mononuclear cells (PBMC).Apoptosis of T lymphocyte was detected by TdT-mediated dUTP nick end labeling(TUNEL) method and flow cytometry assay. Results: The number of CD+ 4 cells (Th2) was significantly increased in asthma group than that in the normal group (P<0.05),and that of the high-dose group was significantly decreased(P<0.05). There was no remarkable difference in the number of CD+ 8 cells (Th1) compared to the normal group (P>0.05). The percentages of apoptosis cells in T lymphocytes were significantly decreased in the asthma group than that in the normal group(P<0.01). The percentages of apoptosis cells in T lymphocytes were significantly increased in the high-dose group(P<0.05). Conclusion: Traditional Chinese herbs Zhixiao Pingchuan prescription can improve the airway inflammation, lowering the numbers of Th2 type cells (CD+4 cells) by enhancing apoptosis of T lymphocyte.
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PAGE：310-312
Experimental study on protective effect of anisodamine on lung ischemia/reperfusion injury in rabbits
WU Hao, CHAO Fu, YANG Hong-sheng， AI Zi-qiang
Department of Thoracic Surgery, the First Hospital of Hangzhou City, Hangzhou 310006, Zhejiang, China.
Abstract:Objective: To explore the protective effects of anisodamine(654-2) on lung and its possible mechanism during lung ischemia-reperfusion injury. Methods: Single lung in suit warm ischemia/reperfusion rabbit model was established. Thirty Japanese rabbits were randomly divided into three equal groups and 10 rabbits were in each group. The control group received no ischemia/reperfusion. The I/R group received ischemia/reperfusion in left lung. The 654-2 group received ischemia/reperfusion in left lung and anisodamine (8 mg/kg) intravenous injection. The changes of lung tissue wet weight/dry weigh ratio(W/D)，malondialdehyde(MDA) and myeloperoxidase(MPO) activity, as well as the lung permeability index(LPI) were checked in each group. The histological appearances of the lungs were observed under histology and electron microscopy. Some sections was examined as an index of quantitative assessment of the degree of lung tissue damage(LTD). Results: After 90 minutes ischemia and 120 minutes reperfusion，W/D，MDA，LPI， MPO activity and LTD degree were significantly higher in I/R group than those of the control group (all P< 0.01, respectively), and the morphology injury of lung tissue was obvious. Anisodamine was effective in reducing W/D， MDA， LPI, MPO activity and LTD (all P<0.01, respectively). Histological examination of lung tissues demonstrated the degree of lung injury had been alleviated significantly. Conclusion: The lung tissue ischemia/reperfusion injury can be significantly protected with anisodamine. The protective mechanism may be that anisodamine can effectively reduce the neutrophil sequestration within the lung and the lung injury resulting from oxygen-derived free radical., 百拇医药
AND WESTERN MEDICINE IN INTENSIVE AND CRITICAL CARE
Volume 10 September 2003 Number 5
PAGE：259-261
Clinical effective observation on integrated traditional Chinese and western medicine in treatment of 51 severe SARS patients
LI Xiu-hui, HU Jian-hua, ZHANG Ke, YE Jun, GOU Chun-yan, LI Yan, ZHANG Bin
, 百拇医药
Beijing Youan Hospital, Beijing 100054, China.
Abstract: Objective: To evaluate the clinical effect of integrated traditional Chinese and western medicine (TCM WM) in treatment of severe SARS patients. Methods: Prognosis and T subset of 51 severe SARS patients admitted to our hospital from March 11 to May 30, 2003 were analyzed. Results: Of 29 cases, 26 cases (86.65%) were cured and 3 cases (10.34%) died in TCM WM group. However, of 22 cases, 12 cases (54.5%) were cured and 10 cases(45.5%) died in western medicine group, it had significance difference (P<0.05 ) between two groups. Clinical symptom improved significantly in TCM WM group, it had significant difference compared with western medicine group (P<0.01). CD+4 T lymphocyte count increased from (361±278)×106/L to (630±454)×106/L in TCM WM group (P<0.05)， and from (288±186)×106/L to (376±285)×106/L in western medicine group(P<0.05). Conclusion: Compared with western medicine treatment, TCM WM treatment can improve clinical symptom and immunity, reduce mortality of severe SARS patients.
, 百拇医药
PAGE：262-264
Dynamic observation on changes of malondialdehyde, nitric oxide and superoxide dismutase in patients with severe acute respiratory syndrome and its significance
ZHUANG Jun-hua, HUANG Xian-zhang, LIN Li, PAN Wan-yi， LIN Lin.
Clinical Laboratory, Ersha Island Branch, Guangdong Provincal Hospital of Traditional Chinese Medicine, Guangzhou 510105, Guangdong, China.
, 百拇医药
Abstract: Objective: To dynamically observe the changes of malondialdehyde (MDA), nitric oxide (NO) and superoxide dismutase (SOD) in patients with severe acute respiratory syndrome(SARS) and to explore its significance. Methods: MDA, NO and SOD were determined by using bathyran chromatometry, nitrate reductase method, xanthine oxidase inhibition method in SARS patients during the first, recovery and follow-up stage, the front health doctors and nurses and health people. The significance of their changes were analyzed. Results: The mean of MDA during the first stage was higher than that of recovery stage and the mean of recovery stage was higher than that of follow-up stage and health people (all P<0.001).The mean of NO during the first stage was higher than that of other stages. The NO means of recovery and follow-up stage were secondly higher. The mean of SOD during the first stage was higher than that of recovery stage and was lower than that of health people(P=0.024), the mean of SOD during the recovery stage was lower than that of follow-up stage, the front health doctors and nurses and health people (all P<0.001). Conclusion: The pathological injury in pathogenesis of SARS is related to free radicals. SOD has the capacity of anti-injury.
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PAGE：265-267
Study on traditional Chinese medical diagnosis and treatment based on differentiation of syndromes and signs in 30 severe acute respiratory syndrome patients
XIAO He-yin, ZHAO Ji-ping, ZHOU Shao-zhong, SHEN Chun, WANG Ji-dong, CUI Hong-sheng, ZHANG Xue-xun, LIU Ying-jie, CHEN Dong-mei, TAO Hong
Dongzhimen Hospital, Beijing University of Traditional Chinese Medicine, Beijing 100700, China.
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Abstract: Objective: To investigate the traditional Chinese medical features of the different symptomatic types of severe acute respiratory syndrome (SARS) and assess the effects of integrated traditional Chinese and western medicine. Methods: According to the different symptomatic types of 30 SARS patients in Dongzhimen Hospital from April 19 to May 1, 2003, they were treated by traditional Chinese medicine herbs based on general treatment including steroid, anti-virus medicine, mechanical ventilation, symptomatic messure and so on. Results: At initial stage 30 cases all had Qi-fen(气分) syndrome such as high fever. They seldom had light fever as Wei-fen(卫分) syndrome. Four cases of them suffered diarrea. As traditional Chinese medicine theory SARS patients had three stages: heat-evil at Qi-fen, flaming of heat-evil at Qi-fen and Ying-fen(营分), heat-evil at Xue-fen(血分). Patients of Qi-fen type were treated with clearing away the heat-evil at Qi-fen, releasing inhabited lung-energy and dissipating phlegm, clearing away heat and toxic materials. The Chinese medicine prescriptions were Wuhu decoction(五虎汤) and Tingli Dazao Xiefei decoction(葶苈大枣泻肺汤). Patients of Qi-Ying-fen(气营分) type were treated with clearing away the heat-evil and phlegm located at Qi-Ying-fen. The Chinese medicine prescriptions were Qingwen Baidu drink(清瘟败毒饮) and Mengshi Guntan decoction(礞石滚痰汤). Patients of Xue-fen type were treated with cooling blood and clearing away the heat-evil, phlegm in the pericardium and waking up the patients from unconsciousness. The Chinese medicine prescriptions were Xijiao Dihuang decoction(犀角地黄汤) and Taohong Siwu decoction (桃红四物汤). Salvia miltiorrhiza injection(丹参注射液) were used in Ying-Xue-fen syndrome. Shengmai injection(生脉注射液) were used in shortage of energy and blood. They were very effective. Up to May 1 among 30 SARS patients, 3 cases recoverd and discharged, 4 cases were dead, the others were transferred to the special hospital, they felt much better. Severe patients had weaning from mechanical ventilation, and the changes in clest X-ray radiogram were improved. Conclusion: According to traditional Chinese medicine theory, SARS belongs to seasonal febrile disease. Its development follows Wei, Qi, Ying, Xue (卫气营血) regulation. The symptom of SARS patients can be obviously improved by the treatment of integrated traditional Chinese and western medicine. The method of activating blood circulation to dissipate blood stasis can be used at every stage.
, 百拇医药
PAGE：268-270
Symptoms of 126 SARS patients in recovering period
CHEN Jing, LIU Wei， YUAN Hui-qing, YU Zhen, YI Jing-hong, WANG Xu-hong, LIU Hui-lin, XUAN Ya-bo, ZHANG Jie, MENG Guang-song
Beijing Hospital of Traditional Chinese Medicine, Beijing 100010, China.
Abstract: Objective: To gather the information of SARS patients who were out of hospital and find their symptoms in order to provide the reasons to treat SARS in recovery period. Methods: The patients of the community in Beijing were inspected, their symptoms, tongue and pulse condition, laboratorial test of blood, result of X-ray and CT were gathered and sumed up. Results: A total of 126 cases were researched, 89 patients were inspected at all. Breathe hard, easy tired, sweat more, dry mouth or bitter taste, feverish sensation, tongue body red, coating thick and greasy, pulse slippery thin and rapid were appeared in more than 47% patients. In about 10 percent-20 percent patients, the results of laboratorial test of blood were abnormal. In 10 percent patients, electrocardiogram were abnormal. Fifty-five cases were found remained shadow in X-ray film. Conclusion: It is necessary to be treated and observed in recovering period in the patients with SARS.
, 百拇医药
PAGE：271-274
Analysis on treatment of severe acute respiratory syndrome by integrated traditional Chinese and western medicine
HE Xiao-yun, HOU Yi-jun, ZHANG Yan-lei, ZHOU Yun-zhi， ZHANG Tao， YUAN Ying, LI Hong-mei
Department of Respiration, The Coal General Hospital, Beijing 100028, China.
Abstract: Objective: To analyze the treatment of serious cases with severe acute respiratory syndrome (SARS) by integrated traditional Chinese and western medicine. Methods: Effect of integrated traditional Chinese and western medicine treatment was reviewed retrospectively on 91 patients of SARS hospitalized in the Coal General Hospital from April 30 to June 5，2003. Results: The mean dosage of glucocorticoid was 153.33 mg/d in treatment group and 246.00 mg/d in control group. The time treating by glucocorticoid was 13.00 days in treatment group and 15.53 days in control group. In patients treated with traditional Chinese and western medicine, the chest X-ray radiogram showed lung fibrosis was ameliorated. It indicated that integrated traditional Chinese and western medicine treatment could reduce the dosage of steroid hormone for serious cases of SARS and shorten time of using steroid hormone in vein. It produced also some effects to prevent SARS patients' condition from worsening and improve the prognosis of serious cases of SARS. Conclusion: Active integrated traditional Chinese and western medicine treatment in the early stage is better in improving the condition, shortening the disease course and reducing the pulmonary fibrosis for SARS patients.
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PAGE：275-278
Effect of naloxone on spinal cord ischemic tolerance induced by pretreatment with repeated electroacupuncture
LEI Yi, XIONG Li-ze, CHEN Shao-yang，HU Bo, ZENG Yi, WANG Qiang, HE Da-yin
Department of Anesthesiology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, Shanxi, China
Abstract: Objective: To study the effect of opioid on spinal cord ischemic tolerance induced by pretreatment with repeated electroacupuncture(EA) in rabbits. Methods: Forty New Zealand white rabbits were randomly divided into five groups (n=8): animals in the control group received no treatment; animals in the sodium pentobarbital(SP) group received SP 30 mg/kg intravenously each day for 5 days; animals in naloxone group received naloxone 0.3 mg/kg intravenously each day for 5 days; animals in EA group were anesthetized with SP 30 mg/kg intravenously and received electroacupuncture either at the Weizhong acupoint(委中穴) 60 minutes each day for 5 days; animals in EA and naloxone group were injected naloxone 0.3 mg/kg intravenously 30 minutes before pretreatment and were anesthetized with SP 30 mg/kg intravenously and received electroacupuncture either at the Weizhong acupoint 60 minutes each day for 5 days. Twenty-four hours after last treatment, spinal cord ischemia was induced by an infrarenal aortic occlusion for 20 minutes. Hind-limb motor function was determined with the Tarlov criteria (in which 4 is normal and 0 is paraplegia) at 4, 8, 12, 24, and 48 hours after reperfusion. All animals were sacrificed at 48 hours after reperfusion and the spinal cords (L5-L7) were removed immediately for histopathologic study. Results: All the animals survived the experiment. The neurologic and histopathologic outcomes in the EA group 48 hours after reperfusion were significantly better than the EA and naloxone group (all P<0.01), but no differences were found among control group, SP group, naloxone group and EA and naloxone group (all P>0.05).Conclusion:The effect of preconditioning with repeated electroacupuncture induces neuroprotection against spinal cord ischemic-reperfusion injury can be restrained by naloxone, which imply opioid relates with the mechanism of repeated electroacupuncture against spinal cord ischemic-preconditioning.
, 百拇医药
PAGE：279-281
Experiment study of salvia miltiorrhiza on inhibiting polymorphonuclear leukocyte and endothelial cells adhesion in acute pancreatitis rabbits
SHA Jian-ping， ZHU Bi-de， XU Yan， CHEN Xuan， ZHAO Yan， LIU Xiao， LU Dian-rong， LIU Zhou
Chengdu University of Tradtional Chinese Medicine, Chengdu 610072, Sichuan, China.
Abstract: Objective: To study the mechanism of polymorphonuclear leukocyte(PMN) and endothelial cell(EC) adhesion in the early period of acute pancreatitis(AP) and the effect of salvia miltiorrhiza on AP. Methods: Rabbit AP model was established. Twenty-four Japan rabbits were divided randomly into normal saline group which was treated with normal saline and the salvia miltiorrhiza treatment group which was treated with saliva miltiorrhiza. Blood was obtained at pro-operation, post-operation instantly, 2 hours, 4 hours, 8 hours, 12 hours, 16 hours and 24 hours after operation. PMN was purred out of the blood, and interacted with rabbit's vascular EC which was cultured out of the body, the adhesion ratio of PMN-EC and CD11a/CD18, CD11b/CD18 expression was measured. Results: After operation, both CD11a/CD18, CD11b/CD18 expression and the PMN-EC adhesion ratio were increased immediately, the normal saline group reached the peak at 4 hours after the operation, the salvia miltiorrhiza treatment group was dramatically lower compared with those of the normal saline group at 2 hours, 4 hours, 8 hours, 12 hours, 16 hours and 24 hours after operation. Conclusion: Salvia miltiorrhiza can lower the CD11a/CD18, CD11b/CD18 expression, inhibit PMN-EC adhesion, improve the microcirculation, ease the tissue damage produced by PMN-EC adhesion and help the AP treatment in the early period.
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PAGE：282-284
Changes of β-endorphin in different tissue of the rabbits with incomplete trauma paraplegia by acupuncture
LIU Jie, HU Xiang-ming, LIU Guang-guo, WANG Qun, LIU Zhi, DING Zhi-gao, ZHANG Guo-min
The First Affiliated Hospital, Hunan College of Traditional Chinese Medicine, Changsha 410007, Hunan, China.
Abstract: Objective: To observe the effect of acupuncture on the change of β-endorphin(β-EP) in the rabbits with incomplete paraplegia, and to explore the biochemical effect of acupuncture to repair of damaged spinal cord. Methods: Acupuncture were applied to governor vessel and nerve trunk for treatment of the rabbits with incomplete paraplegia. The concentration of β-EP in the plasma, pituitary, injuried spinal cord and hypothalamus of the rabbits was determined at 6 hours, 7 days and 15 days. Results: The concentration of β-EP in rabbits after acupuntured once (6 hours ago) decreased significantly compared to the model group. The regulation of β-EP level by acupunture was fast in the blood plasma and pituitary of the rabbits and it descended by stage at 6 hours, 7 days and 15 days. There was a significant difference between 6 hours and 15 days about β-EP levels of hypothalmas. The β-EP level in spinal cord began to descend at 7 days. Conclusion: Acupuncture treatment can facititate the damaged spinal cord repair by degrading and diffusing β-EP, blocking β-EP release, reducing β-EP secretion immediately, thus decreasing the level of β-EP in the blood and reducing β-EP combination with the β-EP receptor of target cells,counteracting the secondary injure of β-EP to spinal cord.
, 百拇医药
PAGE：285-286
Clinical study on treatment with Xuanjiang Feiqi mixture(宣降肺气方) for acute seizure phase of chronic cor pulmonale
YANG Mao-nong, XU Fang-yuan, LIU Yue-jian, CHEN Jin, GAN Jin-hua
The People's Hospital of Sichuan Province, Chengdu 610072, Sichuan, China.
Abstract: Objective: To observe the curative efficacy of Xuanjiang Feiqi mixture(XJFQM,宣降肺气方) combined with western medicine(WM) for acute seizure phase of chronic cor pulmonale. Methods: Sixty-two cases with acute seizure phase of cor pulmonale were randomly divided into two groups. The treatment group(n=31) was treated with both conventional therapy of WM and XJFQM, and the control group(n=31) was treated with conventional WM therapy described above only. Ten days after the treatment, the level of plasma endothelin-1(ET-1) and blood gas indices were detected in both groups, and the therapeutic effects were observed. Results: The markedly effective rate of the treatment group(67.74%) was higher than that of the control group(41.94%,P<0.05). Meanwhile the improvements of plasma ET-1,partial pressure of oxygen in artery(PaO2), partial pressure of carbondioxide in artery (PaCO2) in each group after the treatment were more obvious than that before the treatment(P<0.05 or P<0.01), and the changing extend were more obviously in treatment group than that in control group (all P<0.05). Conclusion: The treatment of XJFQM combined WM for acute seizure phase of chronic cor pulmonale is able to heighten the curative efficacy significantly.
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PAGE：287-289
Effect of Jiawei Zhenwu decoction(加味真武汤) on plasma endothelium in rabbits with congestive heart failure belonged to Yang-deficiency and water-stagnation symptom(阳虚水停证) of Saoyin disease(少阴病)
LONG Xin-sheng, LU Ping, MO Jin-song, LI Fa-la, ZHANG Fei-hui, QIU Xia, WANG Ju-ping, ZHANG Zhi-ling
Shenzhen Hospital of Traditional Chinese Medicine, Shenzhen 518033, Guangdong, China.
, 百拇医药
Abstract: Objective: To study the effect of Jiawei Zhenwu decoction(加味真武汤) on plasma endothelium in rabbits with congestive heart failure (CHF) belonged to Yang-deficiency and water-stagnation symptom(阳虚水停证) of Saoyin disease(少阴病). Methods: By first infusing large dose of cold nature drugs, followed by ligaturing the anterior descending branch of coronary artery and gradually narrowing down their aortic caliber, the rabbit model of Yang-deficiency and water-stagnation symptom of Saoyin disease in CHF was successfully established. The rabbits were divided into four groups: traditional Chinese medicine group was infused Jiawei Zhenwu decoction, western medicine group was treated with captopril, traditional Chinese medicine and western medicine group was treated with Jiawei Zhenwu decoction and captopril, control group was not received medicine. The plasma endothelin was measured in each group. Results: The level of plasma endothelin in the model rabbit was significantly higher than that in normal rabbits(P<0.05). Jiawei Zhenwu decoction could lower the level of plasma endothelin in the model rabbit, there was obviously difference between traditional Chinese medicine group and control group (P<0.01). There was not obviously difference between traditional Chinese medicine group and western medicine group(P>0.05). Conclusion: The low of plasma endothelin level was one of the mechanisms of treatment function of Jiawei Zhenwu decoction on CHF.
, 百拇医药
PAGE：290-292
Clinical observation on the effect of Ganxian prescription(肝纤方) combined lamivudine in treating 31 patients with chronic hepatitis B
SHEN Wei-sheng， YANG Hong-zhi, HONG Qi, ZHANG Yong-qiang, DAI Min
Jiangyin People's Hospital, Jiangyin 214400, Jiangsu, China
Abstract: Objective: To assess the therapeutic effects of Ganxian prescription(肝纤方) combined lamivudine in treating chronic hepatitis B. Methods: Thirty-one patients with chronic hepatitis B in treatment group were treated with Ganxian prescription and lamivudine, while 37 patients in control group were just received lamivudine. The therapeutic duration in both group were one year. Results: The overall effective rate including wholly and partly response was 90.3% in treatment group and 70.3% in control group (P<0.05). No tyrosine-methionioe-aspartate-astartate (YMDD) mutants were found in treatment group but 5 in control group(16.7%). The other objective markers in treatment group were superior to those in control group. Conclusion: Ganxian prescription can offset the limitation of lamivudine. The therapeutic effects can be significantly improved by Ganxian prescription combined lamivudine to treat chronic hepatitis B.
, 百拇医药
PAGE：293-295
Clinical comparison of cerebral vascular spasm treated with Yangxue Qingnao granular(养血清脑颗粒)
ZHANG Jian-jun， DONG Wei-feng, ZHANG Jun， XUAN Hong-fei， GU Shui-jun, XIE Ren-long, SI Yun
Xiaoshan the First People's Hospital, Hangzhou 311201, Zhejiang, China.
Abstract: Objective: To observe the effects of Yangxue Qingnao granular(养血清脑颗粒) in treating cerebral vascular spasm (CVS) caused by different cause. Methods: One hundred and twenty cases with CVS approved by transcraninal Doppler ultrasonography(TCD) were divided randomly into three groups, patients in A group were treated with Yangxue Qingnao granular and basic treatment. Patients in B group were treated with nimodipine and basic treatment. Patients in C group were treated with basic treatment only. The TCD test was repeated and the data of clinical sign were observed and analyzed after 4 weeks. Results: The outcome of treatment to deal with CVS in A and B group was better than that in C group (P<0.01). The outcome of treatment to deal with CVS caused by subarachnoid hemorrhage (SAH) in A group was better than that in B group (P<0.05). Conclusion: Yangxue Qingnao granular has the obvious therapeutic effect in treatment of CVS, especially in CVS which is not caused by SAH.
, 百拇医药
PAGE：296-298
Effect of Lingxie capsule(羚蝎胶囊) on circulating endothelial cells of rats with focal cerebral ischemia/reperfusion injury
ZHU Dong-sheng, MA Zhi-heng, CHEN Yi-wei, ZHU Jun， WANG Fang, WEI Hong-chang, XU Min-hua
Shanghai Second People's Hospital, Shanghai 200011, China.
Abstract: Objective: To observe the effect of Lingxie capsule(羚蝎胶囊) on circulating endothelial cells (CEC) of rats with focal cerebral ischemia/reperfusion(I/R) injury, and to investigate the mechanism of Lingxie capsule in treatment of cerebral infarction. Methods: Thirty-six SD rats were randomly divided into three groups, the sham-operated group, the control group and the Lingxie capsule treatment group. Rat model of cerebral I/R injury was established by the middle cerebral artery occlusion. The neurofunction deficit score and CEC were observed in cerebral I/R model rats. Results: Significant difference was found between the control group and the Lingxie capsule treatment group in decrease of neurofunction deficit score 3 hours after operation 〔(2.30±0.34) scores in Lingxie capsule treatment group and (3.10±0.23) scores in model group, P<0.05〕 and the count of CEC 〔(5.57±0.89) cells/0.9 μl in Lingxie capsule treatment group and (6.38±0.91)cells/0.9 μl in model group, P<0.05〕. Conclusion: Lingxie capsule might play an important role in reducing CEC of rats with focal cerebral I/R injury, and it may be the mechanism of treating cerebral infarction.
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PAGE：299-301
Protective effects of tetramethylpyrazine in patients with acute cerebral ischemia/ reperfusion injury after cardiopulmonary resuscitation
LOU Zheng-jia, ZHUGE Li-min, ZHENG Wen-long, OUYANG Kan, SHOU Lu
Department of Emergency, Hangzhou Hospital of Traditional Chinese Medicine, Hangzhou 310007, Zhejiang, China.
Abstract: Objective: To evaluate the protective effects of tetramethylpyrazine on acute cerebral ischemia/reperfusion injury in patients after cardiopulmonary resuscitation. Methods: Forty-two cases who were going to be treated with cardiopulmonary resuscitation were randomly divided into two groups, 22 cases in treatment group (A group) and 20 cases in control group (B group). Both groups were treated with tetramethyplyrazine 240 mg added 5% glucose 250 ml infused intravenously for 1 hour, once a day for 7 days. The additional treatment of tetramethylpyrazine infusion was administered in A group while in B group was not. The changes of plasma superoxide dismutase (SOD), malondialdehyde (MDA) and thromboxane B2 (TXB2) were determined in each group before and after therapy. The conciousness recovery was as the main index to valuating the clinical curative effects. Results: The marked effective rate, effective rate and no effective rate in A group(72.72%, 13.64%, 13.64%) was significantly higher than those in B group (40.00%, 15.00%， 45.00%, all P<0.05). The plasma SOD, MDA and TXB2 levels were more remarkably improved in A group (all P<0.01). Conclusion: Treating with tetramethylpyrazine can restrain the over oxidation of lipid, bate the conglomeration of platelet and evaluate the activity of SOD，even can elevate the rate of cerebral resuscitation. It can prevent patients from acute cerebral ischemia/reperfusion injury after cardiopulmonary resuscitation.
, 百拇医药
PAGE：302-303
Clinical observation on effect of Puerarin(葛根素) combined with meglumine adenosine cyclophosphate in treatment of refractory heart failure
WANG Yong-jian, GUO Peng, QU Yu-zhen
Emergency Department,the Third People's Hospital of Jinan, Jinan 250101, Shandong, China.
Abstract: Objective: To explore the clinical effect of Puerarin combined with meglumine adenosine cyclophosphate in treatment of refractory heart failure. Methods: Sixty-two cases with refractory heart failure were randomly divided into control group (n=30) and treatment group (n=32). The control group was treated with routine therapy including oxygen inhalation, diuretic, digitalis, vasodilator, angiotensin-converting enzyme inhibitor (ACEI), antibiotic therapy, and supporting treatment. The treatment group was treated with Puerarin 0.4 g+5% glucose 250 ml intravenously drip once a day for 14 days, meglumine adenosine cyclophosphate 120 mg+5% glucose 250 ml intravenously, drip once a day for 14 days, as well as routine therapy. After two weeks, the clinical effects were compared between two groups. Results: The heart function and cardiac indexes were improved after treatment in two groups (all P<0.01), the difference was significant between treatment group and control group after treatment (P<0.05). Conclusion: Combined Puerarin with meglumine adenosine cyclophosphate in treating refractory heart failure is effective and safe.
, 百拇医药
PAGE：304-306
Myocardial injury and changes of nitric oxide content in hemorrhagic shock/ reperfusion and the effect of ganoderma lucidum polysaccharide on them
YANG Hong-mei，WANG Li， CHEN Jie， PEI Rui, XU Qiu-xia, GUO An-qi, GUI Xing-fen. Henan Medical College for Staff and Worker, Zhengzhou 450003, Henan, China.
Abstract: Objective: To investigate the myocardial injury and changes of nitric oxide (NO) content in hemorrhagic shock / reperfusion (HS-R) rabbits and the effect of ganoderma lucidum polysaccharide (GLP) on them. Methods: Using the HS-R model of rabbits, animals were divided into three groups: sham operation group, reperfusion with normal saline (NS) group, reperfusion with 1% GLP group. The mean artery pressure (MAP), ventricular function, NO content in serum and myocardium, the activity of NOS in myocardium were examined. Results: At 40 minutes after hemorrhagic shock, left ventricular end-diastolic pressure(LVEDP) were slightly increased in the two reperfusion groups, but the difference was not significant (all P>0.05). The maximum rate of intraventricular pressure rise (+dp/dtmax) and maximum rate of intraventricular pressure down (-dp/dtmax) were reduced, and NO content in serum were increased significantly compared with those before shock and in sham operation group (all P<0.05). At 40 minutes after reperfusion, ventricular functions were reduced, NO content in serum and myocardium, the activity of NOS in myocardium were all increased significantly in reperfusion with NS group compared with those in sham operation group (all P<0.05). At the same time, the cardiac function was improved, NO concentration in serum and myocardium, the activity of NOS in myocardium were significantly decreased in reperfusion with 1% GLP group than those in reperfusion with NS group (all P<0.05). Conclusion: In HS-R process, the activity of NOS, the NO content in serum and myocardium are all increased, the cardiac function is decreased. The myocardial injury in HS-R is related with changes of NO content. GLP has protective effect on myocardial injury in HS-R .Its mechanism would be inhibiting the activity of NOS and reduced NO content in myocardium.
, 百拇医药
PAGE：307-309
Effects of traditional Chinese herbs Zhixiao Pingchuan prescription(止哮平喘方) on Th1/Th2 cells in guinea-pigs asthma model
HU Zuo-wei, ZHOU Yan-ping, WANG Peng
Institute of Integrated Traditional Chinese and Western Medicine, Fudan University, Shanghai 200040, China.
Abstract: Objective: To investigate the effects of traditional Chinese herbs Zhixiao Pingchuan prescription(止哮平喘方) on Th1/Th2 cells in guinea-pigs asthma model. Methods: CD+ 4 and CD+ 8 T cells counts were detected with immunocytochemistry (ICC) in the peripheral blood mononuclear cells (PBMC).Apoptosis of T lymphocyte was detected by TdT-mediated dUTP nick end labeling(TUNEL) method and flow cytometry assay. Results: The number of CD+ 4 cells (Th2) was significantly increased in asthma group than that in the normal group (P<0.05),and that of the high-dose group was significantly decreased(P<0.05). There was no remarkable difference in the number of CD+ 8 cells (Th1) compared to the normal group (P>0.05). The percentages of apoptosis cells in T lymphocytes were significantly decreased in the asthma group than that in the normal group(P<0.01). The percentages of apoptosis cells in T lymphocytes were significantly increased in the high-dose group(P<0.05). Conclusion: Traditional Chinese herbs Zhixiao Pingchuan prescription can improve the airway inflammation, lowering the numbers of Th2 type cells (CD+4 cells) by enhancing apoptosis of T lymphocyte.
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PAGE：310-312
Experimental study on protective effect of anisodamine on lung ischemia/reperfusion injury in rabbits
WU Hao, CHAO Fu, YANG Hong-sheng， AI Zi-qiang
Department of Thoracic Surgery, the First Hospital of Hangzhou City, Hangzhou 310006, Zhejiang, China.
Abstract:Objective: To explore the protective effects of anisodamine(654-2) on lung and its possible mechanism during lung ischemia-reperfusion injury. Methods: Single lung in suit warm ischemia/reperfusion rabbit model was established. Thirty Japanese rabbits were randomly divided into three equal groups and 10 rabbits were in each group. The control group received no ischemia/reperfusion. The I/R group received ischemia/reperfusion in left lung. The 654-2 group received ischemia/reperfusion in left lung and anisodamine (8 mg/kg) intravenous injection. The changes of lung tissue wet weight/dry weigh ratio(W/D)，malondialdehyde(MDA) and myeloperoxidase(MPO) activity, as well as the lung permeability index(LPI) were checked in each group. The histological appearances of the lungs were observed under histology and electron microscopy. Some sections was examined as an index of quantitative assessment of the degree of lung tissue damage(LTD). Results: After 90 minutes ischemia and 120 minutes reperfusion，W/D，MDA，LPI， MPO activity and LTD degree were significantly higher in I/R group than those of the control group (all P< 0.01, respectively), and the morphology injury of lung tissue was obvious. Anisodamine was effective in reducing W/D， MDA， LPI, MPO activity and LTD (all P<0.01, respectively). Histological examination of lung tissues demonstrated the degree of lung injury had been alleviated significantly. Conclusion: The lung tissue ischemia/reperfusion injury can be significantly protected with anisodamine. The protective mechanism may be that anisodamine can effectively reduce the neutrophil sequestration within the lung and the lung injury resulting from oxygen-derived free radical., 百拇医药