Chin Crit Care Med, January 2004, Vol.16, No.1
PAGE：1
The diagnosis criteria of multiple organ dysfunction syndromes in the elderly (MODSE) (trial draft, 2003)
Wang Shi-wen, Wang Jin-da, Chen Ke-ji, Wang Yi-tang, Wang Xin-de, Mou Shan-chu
General Hospital of People's Liberation Army, Beijing 100853, China
PAGE：2-5
Impact of secondary pancreatic infection on the prognosis of patients with severe acute pancreatitis: an analysis of 60 cases
, 百拇医药
Li Guang-kuo, Tian Fu-zhou, Su Yong-ping, Li Xu
Center of General Surgery, General Hospital of Chengdu Command, Chengdu 610083, Sichuan, China
OBJECTIVE: To investigate the impact of secondary pancreatic infection (SPI) on the prognosis of patients with severe acute pancreatitis (SAP). METHODS: Clinical data of 60 patients with SAP (January 1, 1980-December 31, 1999), especially the data at the onset of SPI, were retrospectively analyzed. Results: Sixty patients were divided into two groups: SPI group (29 cases) and non-SPI group (31 cases). There was no significant difference in gender, average age, scores of Ranson and of high risk factors of China Medical Association between two groups, but the average stay days in hospital, payment, acute physiology and chronic health evaluation II (APACHE II) scores, days of fever, prehospital days, days of high white blood cell (WBC) count, the duration of comstipation after the onset of SAP and average times of operation were significantly higher in SPI group than that of non-SPI group. Fatality rate of SPI group (7/29, 24.14 percent) was higher than that of non-SPI (1/31, 3.23 percent). There were 14 cases of infection with single microorganism (48.3 percent) and 15 cases of mixed infection (51.7 percent) in SPI group. Twenty-seven patients were infected with bacteria of Enterobacteriaecae (Escherichia 25, Klebsiella pneumoniae 1, Proteus morgani 1, 8 with bacteria of Pseudomonas (P. aeruginosa 7, P. stanieri 1), 2 with fungus, 5 with other bacteria (Bacillus subtilis 2, Tetracoccus 1, Acetobacteraceae 1, Staphylococcus epidermidis 1) in SPI group. Only in 2 patients with infective pancreatic necrosis the blood culture was positive (Escherichia coli). CONCLUSION: These results showed that the major source of SPI is entergenous, and SPI would affect the prognosis of SAP patients. With the present therapeutic regimes it was hard to prevent entergenous infections. It is necessary to find a new strategy.
, 百拇医药
PAGE：6-8
Effects evaluation of middle mode artificial liver in treatment of severe hepatitis
He Nian-hai, Wang Ying-jie, Liu Jun, Li Jia-jia, Liu Guo-dong, Wang Yu-ming
Research Institute of Infectious Disease, Southwest Hospital, Third Military Medical University, Chongqing 400038, China
OBJECTIVE: To evaluate the clinical efficacy and safety of middle mode artificial liver-plasma pheresis in treatment of severe hepatitis. METHODS: Seventeen patients with severe hepatitis were treated with plasma pheresis. The results of liver function, renal function, blood routine, prothrombin time (PT), prothrombin time activity (PTa) before and after the treatment were analyzed. All patients were observed closely. Results: Symptoms of patients treated with plasma pheresis were improved, and the total effective rate reached 58.8 percent, but the survival rate was only 11.8 percent. Compared with those before the therapy, there were significant differences in aminotransferase, total bilirubin, direct bilirubin, PT, PTa and total protein level after treatment (P<0.05 or P<0.01). The side-effects were mild. CONCLUSION: Middle artificial liver is effective in the treatment of severe hepatitis.
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PAGE：8
Study on the emergency treatment of 106 acute tetramine poisoning patients
Cheng Yu-zhi, Chen Bo-zeng
Xinzhou People's Hospital, Xinzhou 034000, Shanxi, China
PAGE：9-12
Effects of acute hypoxia and intermittent hypoxic acclimatization on erythropoietin and hypoxia-inducible factor-1α gene expression in rat hepatic and renal tissues
, 百拇医药
Chen Wei， Chen Jia-pei, Ge Shi-li, Cong Yuwen, Fu Xiao-bing
PLA Key Laboratory for Burn and Repair, 304 th Hospital, Beijing 100037, China
OBJECTIVE： To explore the effects of acute hypoxia and intermittent hypoxic acclimatization on gene expression of erythropoietin (EPO) and hypoxia-inducible factor-1α (HIF-1α) in rat hepatic and renal tissues. METHODS: Twenty-four rats (weight was 180 to 220 g) were divided into three groups: normal control group (NC), intermittent hypoxic acclimatization (IH) and acute hypoxia groups (AH). Gene expression of EPO and HIF-1α were examined with Northern dot blot method. Results: As compared with NC group, the levels of EPO gene expression in rat hepatic and renal tissues in AH group were both significantly elevated (P<0.05 or P<0.01). In IH group, the contents of EPO mRNA in hepatic and renal tissues were apparently decreased versus AH group (P<0.01), whilst were not differently elevated in comparison with group NC (P>0.05). In hepatic tissue from AH group, the content of HIF-1α mRNA were more than those in NC group and IH group, while the levels of gene expression of HIF-1α were no substantial change in renal tissues from different groups (all P>0.05). CONCLUSION: Hypoxic acclimatization can inhibit the increment of EPO gene expression induced by acute hypoxia in rat hepatic and renal tissues, in which HIF-1α may play important roles.
, 百拇医药
PAGE：12
Mechanical ventilation for 32 patients with respiratory failure induced by acute poisoning
Li Jia-jia
Kaiyuan People's Hospital, Kaiyuan 661600, Yunnan, China
PAGE：13-18
Effects of fibroblast growth factor-10 on the secretions of transforming growth factor-α, platelet-derived growth factor-AB and vascular endothelial grouth factor by normal adult human keratinocytes in culture
, http://www.100md.com
Sun Xiao-qing, Fu Xiao-bing, Chen Wei, Sun Tong-zhu
304 th Hospital of People's Liberation Army, Beijing 100037, China
OBJECTIVE: To evaluate the effect of fibroblast growth factor-10(FGF-10) on the secretion of transforming growth factor-α (TGF-α), platelet-derived grouth factor-AB (PDGF-AB) and vascular endothelial growth factor (VEGF) by human keratinocytes. METHODS: Concentrations of FGF-10 used were 4, 16, 125 and 500 ng/ml. Serum-free keratinocyte growth medium without EGF or with EGF were as negative control and positive control, respectively. Cells were cultured at 2500， 37 500 cells/cm 2 in dishes in serum-free medium and supernatants were collected at 24, 48 and 72 hours. The concentrations of TGF-α， PDGF-AB and VEGF in cell culture supernatant were measured by using TGF-α, PDGF-AB and VEGF enzyme linked immunoadsorbent assay (ELISA) kits, respectively and cell numbers were counted by haemocytometer. Results: For cells cultured at low density and cells were subconfluent, TGF-α in each group was low and there was no significant difference among them at 24 hours. At 48 hours, both in absolute concentrations and on a per-cell basis, FGF-10 enhanced the secretion of TGF-α by cells cultured at low density in a dose-dependent fashion. At 72 hours, the concentrations of TGF-α in all dose of FGF-10 were significantly higher than that in negative control (P<0.05). The production of TGF-α in pg/10 6cells in 500 ng/ml FGF-10 reached statistical significance compared with negative control (P<0.05). The secretion of TGF-α in FGF-10 were lower than that in positive control at 48 and 72 hours. For cells cuttured at 37500 cells/cm 2, the secretion of TGF-α stimulated by FGF-10 was similar to that when cells cuttured at lower density. PDGF-AB in each group was undetectable. When cells cuttured at high density and reached 100 percent confluent, PDGF-AB could not be detected at 24 hours. At 48 hours, both in absolute concentrations and on a per-cell basis, PDGF-AB productions in 125 and 500 ng/ml of FGF-10 were markedly greater than those of negative control at 48 and 72 hours (P<0.05), and the secretion of PDGF-AB stimulated by FGF-10 was in a dose-dependent fashion. For cells cultured at low density, although the concentrations of VEGF in 16-500 ng/ml of FGF-10 were significantly higher than that in negative control (P<0.05) at 72 hours, on a per-cell basis, they were not greater than that of negative control. The secretion of VEGF in positive control was greater than that of FGF-10 at 24-72 hours. CONCLUSION: The effect of FGF-10 on increasing secretion of TGF-α and PDGF-AB might mediate the actions of FGF-10 on keratinocytes, fibroblasts and endothelial cells to promote re-epithelialization as well as granulation tissue deposition during wound healing.
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PAGE：19-21
Effects of acidi fibroblast growth factor on hepatic and renal functions after intestinal ischemia/reperfusion injury
Weng Li-xin, Fu Xiao-bing, Li Xiu-xia, Sun Tong-zhu, Zheng Shu-yun, Chen Wei.304 th Hospital of PLA, Beijing 100037, China
OBJECTIVE： To investigate the change in hepatic and renal functions parameters after intestinal ischemia-reperfusion (I/R) injury, and to explore the effects of acidic fibroblast growth factor (aFGF) on hepatic and renal functions after intestinal I/R injury in rats. METHODS: Seventy-eight Wistar rats were divided into four groups, which are sham-operated (C) group, ischemia (45 minutes) plus reperfusion (R), reconstructive human aFGF treatment (rhF), and wild type aFGF treatment (wtF) groups. The animals were sacrificed at 2, 6, 12 and 24 hours, respectively. Hepatic and renal functions were analyzed. Results: In comparison with those in group C, the hepatic and renal functions were damaged in group R, rhF and wtF decreased. Treatment with rhF and wtF markedly abated the hepatic and renal dysfunction. The desquamation of intestine villi and infiltration of inflammation cells in the submucosa were observed in all groups. CONCLUSION: Hepatic and renal functions are damaged after intestinal I/R injury. Treatment with rhF and wtF could protect against multiple organ dysfunction associcated with intestinal ischemia-reperfusiun injury.
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PAGE：22-25
Radioprotection of recombinant human interleukin-3 and granulocyte-macrophage colony-stimulating factor on peripheral lymphocytes of rhesus monkey irradiated by 3.0 Gy γ-rays
Cui Yu-fang, Yang Hong, Luo Qing-liang, Dong Bo, Liu Xiao-lan, Xu Han, Mao Bing-zhi, Wang De-wen
Beijing Institute of Radiation Medicine, Military Academy Medical Sciences, Beijing 100850, China
, http://www.100md.com
OBJECTIVE： To observe the radioprotection of recombinant human interleukin-3 (rhIL-3), granulocyte-macrophage colony-stimulating factor(GM-CSF) and rhIL-3 (rhIL-3+GM-CSF) on peripheral lymphocytes of rhesus monkey irradiated by 3.0 Gy γ-rays, and attempt to provide evidence of cytokines used effectively in the therapy of acute radiation sickness. METHODS: Thirty rhesus monkey used in the experiment were randomly divided into six groups of rhIL-3 20μg·kg -1·d -1, 60μg·kg -1·d -1， GM-CSF 10μg·kg -1·d -1，IL-3 20 μg·kg -1·d -1 +GM-CSF 10μg·kg -1·d -1， radiation control and normal control. 21 d after whole body γ-irradiation and subcutaneous injection of cytokines, T lymphocyte and its subsets, Bax/Bcl-2 proteins in lymphocytes were determined by immunohistochemical staining with alkaline phosphatase, and lymphocyte apoptosis was detected by TdT-mediated dUTP nick end labeling (TUNEL) technique. Results: (1) After irradiation the quantities of peripheral lymphocyte, T cell and its subsets obviously decreased as compared with those of normal controls. For instance, the percentages of lymphocyte, T, T H and Ts cells in radiation control group reduced to 44 percent, 42 percent, 41 percent and 57 percent of normal controls, respectively. (2)After radiation the reduction of lymphocyte, T, T H and Ts cells were evidently improved by injection of GM-CSF and GM-CSF+IL-3， The T,T H cells in GM-CSF and GM-CSF+IL-3 groups were respectively elevated by 1.57 and 1.76 fold, as well as 1.48 and 1.72 fold of radiation controls. (3) A large amount of lymphocyte apoptosis was found after radiation, GM-CSF and GM-CSF+IL-3 treatment could distinctively inhibit abundant lymphocyte apoptosis induced by acute irradiation,the apoptotic rates of lymphocytes in GM-CSF and GM-CSF+IL-3 groups reduced to 41 percent and 48 percent respectively when compared with that of radiation controls. CONCLUSION: A definite dose of GM-CSF and GM-CSF+IL-3 could suppress the reduction of lymphocyte, T and T H cells and lymphocyte apoptosis induced by 3.0 Gy γ-irradiation. It confirms that inhibition of GM-CSF and GM-CSF+IL-3 on lymphocyte reduction as well as apoptosis might be one of the major causes to alleviate radiation injury of lymphocytes and improve the immunological function.
, 百拇医药
PAGE：26-28
Experimental study on prevention and treatment of focal segmental glomerulosclerosis with heparin
Zhang Bi-li， Li Zhi-jun, Song Lan-yun， Zhou Hong, Wang Wen-hong, Zhang Xuan Department of Nephrology, Tianjin Children's Hospital, Tianjin 300074, China
OBJECTIVE： To observe the preventive and therapeutic feasibility of heparin calcium injection in rats with focal segmental glomerulosclerosis (FSGS). METHODS: Eighteen male Wistar rats were divided into three groups： Normal control group (A, n=6), heparin-treated rats (B, n=6) and model group (C, n=6). Rat model of FSGS was constructed with low-dose adriamycin, metaraminol and high lipid feed method. Group B was treated with heparin. 24-hour urinary protein was measured at the 0, 2nd, 4th, 6th and 12th week. Serum total protein, albumin, cholesterol, blood urea nitrogen (BUN) and serum creatinine (SCr) were determined at 0, 2nd, 6th and 12th week. Renal cortex blood flow was performed at the 12th week. Renal pathology changes were evaluated at the 12th week as well. Results: 24-hour urinary protein was decreased in heparin treatment group after 6th week. The serum cholesterol level in heparin treatment group was lower than that in model group at 12th week. Renal cortex blood flow was increased significantly in heparin-treated group. Glomerular sclerotic index (SI) and glomerular extracellular matrix (ECM)/glomerular area (GA) were decreased significantly in heparin treatment group. Electron microscopy revealed that there was no glomerular capillary cavities obstruction in heparin -treated group, basement membrane change and foot precesses fusion were markedly alleviated in comparison with model group. CONCLUSION: Heparin may be playing a valuable role in the prevention and treatment of FSGS.
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PAGE：29-32
Effect of fructose-1,6-diphosphate and dexamethasone on ischemia/reperfusion injury after hemorrhagic shock in rabbits
Chen Yong-quan, Jin Xiao-ju, Tang Rong-hua, Huang Wen-bin, Tao Qing-song, Wang Bang-an
Yijishan Hospital, College of Wannan Medical, Wuhu 241001, Anhui, China
OBJECTIVE： To investigate the mechanism of myocardial ischemia/reperfusion injury and the protective effect of fructose-1, 6-diphosphagic (FDP) and dexamethasone (DXM) in hemorrhagic shock in rabbits. METHODS: Using a hemorrhagic shock model of Wiggers, 48 rabbits were randomly divided into 6 groups. GroupⅠ control group; GroupⅡ with drugs given before ischemia phase (divided into 3 groups: FDPⅠ, DXMⅠ and FDPⅠ+ DXMⅠ); Group Ⅲ with drugs given in reperfusion phase (divided into 2 groups: FDPII and DXMⅡ). The levels of creatine kinase (CK) and troponin I (cTnI) in plasma were measured, and myocyte apoptosis index was assessed. Results: Baseline levels of CK and cTnI were similar in three groups; CK and cTnI and apoptosis index were lower or with a lower tendency in groupⅡ and in groupⅢ (P<0.05 or P<0.01); CK and cTnI showed a lower tendency in rise in FDPⅠ and DXMⅠ than in FDPII and even slower in FDP group than in DXM group; CK and cTnI levels rose slower in FDPⅠ+DXMⅠ than in FDPⅠ and DXMⅠ.CONCLUSION: FDP given during ischemia and DXM could effectively protect the myocardium from reperfusion injury following hemorrhagic shock.
, 百拇医药
PAGE：33-35
Significance of Changes in plasma lipid peroxide levels in critical lyill patients after liver transplantation
Liu Chun, Zhou Xiao-si, Geng Qiu-ming
The Third Hospital of Peking University,Beijing 100083, China; Corresponding author: LIU Chun' Tianjin Union Hospital, Tianjing 300050, China
OBJECTIVE： To investigate the relationship between plasma lipid peroxide (LPO) levels and prognosis in critically ill patients after liver transplantation. METHODS: Plasma LPO levels were measured in 18 patients within 21 days after liver transplantation, and their relations to the prognosis were analyzed. Results: Plasma LPO levels were markedly elevated in patients before state of illness got severe, especially in those with fatal outcome. The peak LPO levels were much higher in non-survivors than in survivors, and mortality rate in patients whose plasma LPO exceeding 10 μmol/L (5/6 cases) was significantly higher than those under 10 μmol/L (1/12 cases, P<0.01). The mortality rate in patients with daily fluctuation range of plasma LPO levels over 1.2 μmol·L-1·d-1 was higher than those under 1.2 μmol· L-1· d-1, and the difference was significant between two groups (P<0.01). CONCLUSION: Measurement of plasma LPO level might be of clinical significance to properly judge the critically ill conditions, and it can be used as routine examination for patients after liver transplantation.
, 百拇医药
PAGE：36-41
Study of mechanism on loss of some components from basement membrane in epithelial-interstitial junction in cutaneous pseudoepitheliomatous hyperplasia lesion
JIANG Du-yin, FU Xiao-bing, SHENG Zhi-yong, CHENG Wei, ZHOU Gang, SUN Tong-zhu Key Laboratory of Wound Repair, 304th Hospital of PLA, Beijing, 100037, China
OBJECTIVE： To investigate the relationship between the formation of pseudoepitheliomatous hyperplasia (PEH) and the mechanism of loss of some components from the basement membrane in epithelial interstitial junction (EIJ) in the treatment of cutaneous wounds, when formation of PEH lesion was induced. METHODS: Morphological change in epithelial tissue was observed with histopathologic method and electronic microscopy in 11 specimens of PEH lesions and 6 specimens of normal skin adjacent to PEH (PEH-N) from 11 patients with injured skin. The expression characteristics and distribution of pan-cytokeratin (p-CK), matrix metalloproteinase-2 (MMP-2), MMP-3, MMP-9, proliferating cell nuclear antigen (PCNA), epithelial cadherin(E-Cad) and β-catenin(β-Cat) in EMJ were detected with immunohistochemical methods. Results: Epithelial cells expressing P-CK presented squamous epithelization and extended into deep layer of mesenchyma. In epithelium-mesenchyma junction, where Ⅳ type collagen and laminin were weakly expressed, the protein contents of p-CK, E-Cad, MMP-2, MMP-3， MMP-9 were decreased, whilst the immunochemical staining of β-Cat and PCNA was apparently increased. In the junction, epithelial basal cells were observed to migrate and to depart from basal membrane; epithelial islands and isolated epithelial cells expressing p-CK in mesenchyma could be observed. Ultrastructural observation revealed deformation of epithelial basal cells, increment of nucleus/cytoplasm ratio, loosened intercellular junctions, decrement of electronic density of BM and derangement of BM stucture could be observed. CONCLUSION: Reduction in the capability of epithelial basal cells adhesion, differentiation and formation of basement membrane and cytokeratin in PEH associated with wound may be the crucial cause which controls epithelial cells migration into mesenchyma. That the contents of ColⅣ and LN were decreased may not be associated with MMPs, but with enhancement of the ratio of β-Cat/E-Cad signal might be the important mechanism of dedifferentiation of epithelial basal cells and the loss of ability of structure formation and cellular migration.
, 百拇医药
PAGE：42-44
Protective effect of propofol on liver during ischemia-reperfusion injury in patients undergoing liver surgery
Lin Li-na, Wang Wan-tie, Wu Jin-ze, Hu Zheng-yang, Xie Ke-jian
Department of Anesthesiology, First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325003, Zhejiang, hina
OBJECTIVE： To explore the protective effect of propofol on liver during hepatic ischemia/reperfusion injury (HIRI) and its mechanisms in patients undergoing liver surgery. METHODS: Eighteen patients who were scheduled for selective hepatic surgery were randomly divided into control group (n=9) and propofol treatment group (n=9). Changes of several parameters in plasma and effects of propofol on them were observed before liver ischemia, at end of ischemia and at reperfusion for 25 minutes, parameters of which included superoxide dismutase (SOD), xanthine oxidase (XO), lipid peroxide (LPO) and alanine aminotrasferase (ALT) activity, and the ultrastructure changes in liver tissue were observed under electron microscope at 25 minutes after reperfusion. Results: SOD activity decreased remarkably (P<0.01); XO activity, LPO concentration and ALT value increased significantlly (P<0.01) during HIRI, and there were abnormal changes of the hepatic ultrastructure at 25 minutes after reperfusion. Afer treatment with propofol, the variation of all parameters were alleviated markedly (P<0.05 and P<0.01). CONCLUSION: Propofol has protective effects on HIRI by reducing oxygen free radical level and inhibiting lipid peroxidation after hepatic ischemia/reperfusion in patients undergoing liver cancer surgery.
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PAGE：45-48
Influence of inhibitors of nitric oxide synthase and L-arginine on the survival rate in a rat model of traumatic shock
HUANG Zong-hai, FENG Hao-miao, HUANG Xu-liang, SUN Ying-gang, LIN Hong-wu
Department of General Surgery， Zhujiang Hospital， First Military Medical University, Guangzhou 510282, Guangdong, China
OBJECTIVE： To investigate the influence of inhibitors of nitric oxide synthase (NOS) and L-arginine on the survival rate in a rat model of traumatic shock. METHODS: A rat model of traumatic shock was established by facturing the posterior limb of the rat. Nω-nitro-L-arginine-methyl ester (L-NAME, non-selective NOS inhibitor, 10 mg/kg), amino- guanidine〔AG, selective inducible nitric oxide synthase (iNOS) inhibitor, 100 mg/kg〕 and L-arginine 〔L-Arg, the precursor of nitric oxide(NO) synthesis 100 mg/kg〕 were injected intravenously during resuscitation,survival time and survival rate were observed. Results: There were no significant changes in survival time and 24-hour survival rate between L-NAME 〔(23.80±9.09) hours and 40 percent〕 and control group〔(18.78±4.64)hours and 10 percent, both P>0.05〕; the survival time of AG group(28.72±6.25)hours and L-Arg group (30.64±8.77)hours prolonged apparently (both P<0.01), and 24-hour survival rate was also increased (both 80 percent, both P<0.01). CONCLUSION: Selective iNOS inhibitor AG and L-Arg exert benefitial effects on after traumatic shock rats.
, 百拇医药
PAGE：49-51
Effects of L-arginine on the function of platelet aggregation during hepatic ischemia/reperfusion injury
Wang Wan-tie, Lin Li-na, Pan Xue-rong, Xu Zheng-jie
Department of Pathophysiology, Wenzhou Medical College, Wenzhou 325027, Zhejiang, China
OBJECTIVE： To explore the effects of L-arginine (L-Arg) on the function of platelet aggregation during hepatic ischemia-reperfusion injury (HIRI). METHODS: The changes in maximum aggregating rate of circulating platelets (Ptmax), its maximum aggregating time (PtT) as well as its aggregating slopes (PtS) were measured. Effects of L-Arg on those parameters were observed during HIRI in 20 rabbits and 18 patients who were scheduled for elective hepatic surgery. Results: Ptmax and PtS all increased significantly (P<0.05 and P<0.01), while PtT decreased remarkably (both P<0.05) during HIRI of rabbits and patients. After treatment with L-Arg, the abnormal changes of parameters as above were all alleviated remarkably (P<0.05 and P<0.01). CONCLUSION: It is indicated that L-Arg can effectively regutate the function of platelet aggregation during HIRI.
, 百拇医药
PAGE：52-53
Effect of complex nutrients on lipid peroxidation reation in stressed rats with moist-heat combired injury
Li Ya-jie, Wang Ying, Zhai Hui-min, Xu Cai-xia, Luo Bing-de
Nanfang Hospital, First Military Medical University, Guangzhou 510515， Guangdong, China
PAGE：54-55
Change in myokinase in hypokalenice periodic paralysis and its diagnostic significance
, 百拇医药
Tu Qiu-yun, Zi Xiao-hong, Li Xiao-bo, Li Yong-hong, Chen Li-hao
The third Xiangya Medical University, Central-South University, Changsha 410013, Hunan, China
PAGE：55-56
Clinical study on charges in serum enzynce and c-reative protein levels in patients with acute poisoning
Wang Yu-jun, Lin Kai-yan, Wu Da-hong, Wang Zheng-ying, Wang Qiong, Mi Xiao-wei, Liu Zhao-hui
, 百拇医药
Qiannan People's Hospital, Qiannan 558000, Guizhou, China
PAGE：57
Significance of procalcitonin in acute pancreatitis
Pei Hong-hong, Feng Ying-qun, Yang Zheng-an, Niu Fei, Wang Ni
Xian traffic university, the second Hospital, Xian 710004, Shanxi, China
PAGE：59-62
Mitogen-activated protein kinase signaling pathway and mucosal repair after intestinal injury
, 百拇医药
Zhen Shu-yun, Fu Xiao-bing, Xu Jian-guo
304th Hospital of PLA, Beijing, 100037, China
PAGE：63-64
Guidelines of European Heart Association: Principles of Emergoncy treatment
Zhu Hai-yan, Shen Hong
General Hospital of People's Liberation Army, Beijing 100853, China, 百拇医药
The diagnosis criteria of multiple organ dysfunction syndromes in the elderly (MODSE) (trial draft, 2003)
Wang Shi-wen, Wang Jin-da, Chen Ke-ji, Wang Yi-tang, Wang Xin-de, Mou Shan-chu
General Hospital of People's Liberation Army, Beijing 100853, China
PAGE：2-5
Impact of secondary pancreatic infection on the prognosis of patients with severe acute pancreatitis: an analysis of 60 cases
, 百拇医药
Li Guang-kuo, Tian Fu-zhou, Su Yong-ping, Li Xu
Center of General Surgery, General Hospital of Chengdu Command, Chengdu 610083, Sichuan, China
OBJECTIVE: To investigate the impact of secondary pancreatic infection (SPI) on the prognosis of patients with severe acute pancreatitis (SAP). METHODS: Clinical data of 60 patients with SAP (January 1, 1980-December 31, 1999), especially the data at the onset of SPI, were retrospectively analyzed. Results: Sixty patients were divided into two groups: SPI group (29 cases) and non-SPI group (31 cases). There was no significant difference in gender, average age, scores of Ranson and of high risk factors of China Medical Association between two groups, but the average stay days in hospital, payment, acute physiology and chronic health evaluation II (APACHE II) scores, days of fever, prehospital days, days of high white blood cell (WBC) count, the duration of comstipation after the onset of SAP and average times of operation were significantly higher in SPI group than that of non-SPI group. Fatality rate of SPI group (7/29, 24.14 percent) was higher than that of non-SPI (1/31, 3.23 percent). There were 14 cases of infection with single microorganism (48.3 percent) and 15 cases of mixed infection (51.7 percent) in SPI group. Twenty-seven patients were infected with bacteria of Enterobacteriaecae (Escherichia 25, Klebsiella pneumoniae 1, Proteus morgani 1, 8 with bacteria of Pseudomonas (P. aeruginosa 7, P. stanieri 1), 2 with fungus, 5 with other bacteria (Bacillus subtilis 2, Tetracoccus 1, Acetobacteraceae 1, Staphylococcus epidermidis 1) in SPI group. Only in 2 patients with infective pancreatic necrosis the blood culture was positive (Escherichia coli). CONCLUSION: These results showed that the major source of SPI is entergenous, and SPI would affect the prognosis of SAP patients. With the present therapeutic regimes it was hard to prevent entergenous infections. It is necessary to find a new strategy.
, 百拇医药
PAGE：6-8
Effects evaluation of middle mode artificial liver in treatment of severe hepatitis
He Nian-hai, Wang Ying-jie, Liu Jun, Li Jia-jia, Liu Guo-dong, Wang Yu-ming
Research Institute of Infectious Disease, Southwest Hospital, Third Military Medical University, Chongqing 400038, China
OBJECTIVE: To evaluate the clinical efficacy and safety of middle mode artificial liver-plasma pheresis in treatment of severe hepatitis. METHODS: Seventeen patients with severe hepatitis were treated with plasma pheresis. The results of liver function, renal function, blood routine, prothrombin time (PT), prothrombin time activity (PTa) before and after the treatment were analyzed. All patients were observed closely. Results: Symptoms of patients treated with plasma pheresis were improved, and the total effective rate reached 58.8 percent, but the survival rate was only 11.8 percent. Compared with those before the therapy, there were significant differences in aminotransferase, total bilirubin, direct bilirubin, PT, PTa and total protein level after treatment (P<0.05 or P<0.01). The side-effects were mild. CONCLUSION: Middle artificial liver is effective in the treatment of severe hepatitis.
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PAGE：8
Study on the emergency treatment of 106 acute tetramine poisoning patients
Cheng Yu-zhi, Chen Bo-zeng
Xinzhou People's Hospital, Xinzhou 034000, Shanxi, China
PAGE：9-12
Effects of acute hypoxia and intermittent hypoxic acclimatization on erythropoietin and hypoxia-inducible factor-1α gene expression in rat hepatic and renal tissues
, 百拇医药
Chen Wei， Chen Jia-pei, Ge Shi-li, Cong Yuwen, Fu Xiao-bing
PLA Key Laboratory for Burn and Repair, 304 th Hospital, Beijing 100037, China
OBJECTIVE： To explore the effects of acute hypoxia and intermittent hypoxic acclimatization on gene expression of erythropoietin (EPO) and hypoxia-inducible factor-1α (HIF-1α) in rat hepatic and renal tissues. METHODS: Twenty-four rats (weight was 180 to 220 g) were divided into three groups: normal control group (NC), intermittent hypoxic acclimatization (IH) and acute hypoxia groups (AH). Gene expression of EPO and HIF-1α were examined with Northern dot blot method. Results: As compared with NC group, the levels of EPO gene expression in rat hepatic and renal tissues in AH group were both significantly elevated (P<0.05 or P<0.01). In IH group, the contents of EPO mRNA in hepatic and renal tissues were apparently decreased versus AH group (P<0.01), whilst were not differently elevated in comparison with group NC (P>0.05). In hepatic tissue from AH group, the content of HIF-1α mRNA were more than those in NC group and IH group, while the levels of gene expression of HIF-1α were no substantial change in renal tissues from different groups (all P>0.05). CONCLUSION: Hypoxic acclimatization can inhibit the increment of EPO gene expression induced by acute hypoxia in rat hepatic and renal tissues, in which HIF-1α may play important roles.
, 百拇医药
PAGE：12
Mechanical ventilation for 32 patients with respiratory failure induced by acute poisoning
Li Jia-jia
Kaiyuan People's Hospital, Kaiyuan 661600, Yunnan, China
PAGE：13-18
Effects of fibroblast growth factor-10 on the secretions of transforming growth factor-α, platelet-derived growth factor-AB and vascular endothelial grouth factor by normal adult human keratinocytes in culture
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Sun Xiao-qing, Fu Xiao-bing, Chen Wei, Sun Tong-zhu
304 th Hospital of People's Liberation Army, Beijing 100037, China
OBJECTIVE: To evaluate the effect of fibroblast growth factor-10(FGF-10) on the secretion of transforming growth factor-α (TGF-α), platelet-derived grouth factor-AB (PDGF-AB) and vascular endothelial growth factor (VEGF) by human keratinocytes. METHODS: Concentrations of FGF-10 used were 4, 16, 125 and 500 ng/ml. Serum-free keratinocyte growth medium without EGF or with EGF were as negative control and positive control, respectively. Cells were cultured at 2500， 37 500 cells/cm 2 in dishes in serum-free medium and supernatants were collected at 24, 48 and 72 hours. The concentrations of TGF-α， PDGF-AB and VEGF in cell culture supernatant were measured by using TGF-α, PDGF-AB and VEGF enzyme linked immunoadsorbent assay (ELISA) kits, respectively and cell numbers were counted by haemocytometer. Results: For cells cultured at low density and cells were subconfluent, TGF-α in each group was low and there was no significant difference among them at 24 hours. At 48 hours, both in absolute concentrations and on a per-cell basis, FGF-10 enhanced the secretion of TGF-α by cells cultured at low density in a dose-dependent fashion. At 72 hours, the concentrations of TGF-α in all dose of FGF-10 were significantly higher than that in negative control (P<0.05). The production of TGF-α in pg/10 6cells in 500 ng/ml FGF-10 reached statistical significance compared with negative control (P<0.05). The secretion of TGF-α in FGF-10 were lower than that in positive control at 48 and 72 hours. For cells cuttured at 37500 cells/cm 2, the secretion of TGF-α stimulated by FGF-10 was similar to that when cells cuttured at lower density. PDGF-AB in each group was undetectable. When cells cuttured at high density and reached 100 percent confluent, PDGF-AB could not be detected at 24 hours. At 48 hours, both in absolute concentrations and on a per-cell basis, PDGF-AB productions in 125 and 500 ng/ml of FGF-10 were markedly greater than those of negative control at 48 and 72 hours (P<0.05), and the secretion of PDGF-AB stimulated by FGF-10 was in a dose-dependent fashion. For cells cultured at low density, although the concentrations of VEGF in 16-500 ng/ml of FGF-10 were significantly higher than that in negative control (P<0.05) at 72 hours, on a per-cell basis, they were not greater than that of negative control. The secretion of VEGF in positive control was greater than that of FGF-10 at 24-72 hours. CONCLUSION: The effect of FGF-10 on increasing secretion of TGF-α and PDGF-AB might mediate the actions of FGF-10 on keratinocytes, fibroblasts and endothelial cells to promote re-epithelialization as well as granulation tissue deposition during wound healing.
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PAGE：19-21
Effects of acidi fibroblast growth factor on hepatic and renal functions after intestinal ischemia/reperfusion injury
Weng Li-xin, Fu Xiao-bing, Li Xiu-xia, Sun Tong-zhu, Zheng Shu-yun, Chen Wei.304 th Hospital of PLA, Beijing 100037, China
OBJECTIVE： To investigate the change in hepatic and renal functions parameters after intestinal ischemia-reperfusion (I/R) injury, and to explore the effects of acidic fibroblast growth factor (aFGF) on hepatic and renal functions after intestinal I/R injury in rats. METHODS: Seventy-eight Wistar rats were divided into four groups, which are sham-operated (C) group, ischemia (45 minutes) plus reperfusion (R), reconstructive human aFGF treatment (rhF), and wild type aFGF treatment (wtF) groups. The animals were sacrificed at 2, 6, 12 and 24 hours, respectively. Hepatic and renal functions were analyzed. Results: In comparison with those in group C, the hepatic and renal functions were damaged in group R, rhF and wtF decreased. Treatment with rhF and wtF markedly abated the hepatic and renal dysfunction. The desquamation of intestine villi and infiltration of inflammation cells in the submucosa were observed in all groups. CONCLUSION: Hepatic and renal functions are damaged after intestinal I/R injury. Treatment with rhF and wtF could protect against multiple organ dysfunction associcated with intestinal ischemia-reperfusiun injury.
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PAGE：22-25
Radioprotection of recombinant human interleukin-3 and granulocyte-macrophage colony-stimulating factor on peripheral lymphocytes of rhesus monkey irradiated by 3.0 Gy γ-rays
Cui Yu-fang, Yang Hong, Luo Qing-liang, Dong Bo, Liu Xiao-lan, Xu Han, Mao Bing-zhi, Wang De-wen
Beijing Institute of Radiation Medicine, Military Academy Medical Sciences, Beijing 100850, China
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OBJECTIVE： To observe the radioprotection of recombinant human interleukin-3 (rhIL-3), granulocyte-macrophage colony-stimulating factor(GM-CSF) and rhIL-3 (rhIL-3+GM-CSF) on peripheral lymphocytes of rhesus monkey irradiated by 3.0 Gy γ-rays, and attempt to provide evidence of cytokines used effectively in the therapy of acute radiation sickness. METHODS: Thirty rhesus monkey used in the experiment were randomly divided into six groups of rhIL-3 20μg·kg -1·d -1, 60μg·kg -1·d -1， GM-CSF 10μg·kg -1·d -1，IL-3 20 μg·kg -1·d -1 +GM-CSF 10μg·kg -1·d -1， radiation control and normal control. 21 d after whole body γ-irradiation and subcutaneous injection of cytokines, T lymphocyte and its subsets, Bax/Bcl-2 proteins in lymphocytes were determined by immunohistochemical staining with alkaline phosphatase, and lymphocyte apoptosis was detected by TdT-mediated dUTP nick end labeling (TUNEL) technique. Results: (1) After irradiation the quantities of peripheral lymphocyte, T cell and its subsets obviously decreased as compared with those of normal controls. For instance, the percentages of lymphocyte, T, T H and Ts cells in radiation control group reduced to 44 percent, 42 percent, 41 percent and 57 percent of normal controls, respectively. (2)After radiation the reduction of lymphocyte, T, T H and Ts cells were evidently improved by injection of GM-CSF and GM-CSF+IL-3， The T,T H cells in GM-CSF and GM-CSF+IL-3 groups were respectively elevated by 1.57 and 1.76 fold, as well as 1.48 and 1.72 fold of radiation controls. (3) A large amount of lymphocyte apoptosis was found after radiation, GM-CSF and GM-CSF+IL-3 treatment could distinctively inhibit abundant lymphocyte apoptosis induced by acute irradiation,the apoptotic rates of lymphocytes in GM-CSF and GM-CSF+IL-3 groups reduced to 41 percent and 48 percent respectively when compared with that of radiation controls. CONCLUSION: A definite dose of GM-CSF and GM-CSF+IL-3 could suppress the reduction of lymphocyte, T and T H cells and lymphocyte apoptosis induced by 3.0 Gy γ-irradiation. It confirms that inhibition of GM-CSF and GM-CSF+IL-3 on lymphocyte reduction as well as apoptosis might be one of the major causes to alleviate radiation injury of lymphocytes and improve the immunological function.
, 百拇医药
PAGE：26-28
Experimental study on prevention and treatment of focal segmental glomerulosclerosis with heparin
Zhang Bi-li， Li Zhi-jun, Song Lan-yun， Zhou Hong, Wang Wen-hong, Zhang Xuan Department of Nephrology, Tianjin Children's Hospital, Tianjin 300074, China
OBJECTIVE： To observe the preventive and therapeutic feasibility of heparin calcium injection in rats with focal segmental glomerulosclerosis (FSGS). METHODS: Eighteen male Wistar rats were divided into three groups： Normal control group (A, n=6), heparin-treated rats (B, n=6) and model group (C, n=6). Rat model of FSGS was constructed with low-dose adriamycin, metaraminol and high lipid feed method. Group B was treated with heparin. 24-hour urinary protein was measured at the 0, 2nd, 4th, 6th and 12th week. Serum total protein, albumin, cholesterol, blood urea nitrogen (BUN) and serum creatinine (SCr) were determined at 0, 2nd, 6th and 12th week. Renal cortex blood flow was performed at the 12th week. Renal pathology changes were evaluated at the 12th week as well. Results: 24-hour urinary protein was decreased in heparin treatment group after 6th week. The serum cholesterol level in heparin treatment group was lower than that in model group at 12th week. Renal cortex blood flow was increased significantly in heparin-treated group. Glomerular sclerotic index (SI) and glomerular extracellular matrix (ECM)/glomerular area (GA) were decreased significantly in heparin treatment group. Electron microscopy revealed that there was no glomerular capillary cavities obstruction in heparin -treated group, basement membrane change and foot precesses fusion were markedly alleviated in comparison with model group. CONCLUSION: Heparin may be playing a valuable role in the prevention and treatment of FSGS.
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PAGE：29-32
Effect of fructose-1,6-diphosphate and dexamethasone on ischemia/reperfusion injury after hemorrhagic shock in rabbits
Chen Yong-quan, Jin Xiao-ju, Tang Rong-hua, Huang Wen-bin, Tao Qing-song, Wang Bang-an
Yijishan Hospital, College of Wannan Medical, Wuhu 241001, Anhui, China
OBJECTIVE： To investigate the mechanism of myocardial ischemia/reperfusion injury and the protective effect of fructose-1, 6-diphosphagic (FDP) and dexamethasone (DXM) in hemorrhagic shock in rabbits. METHODS: Using a hemorrhagic shock model of Wiggers, 48 rabbits were randomly divided into 6 groups. GroupⅠ control group; GroupⅡ with drugs given before ischemia phase (divided into 3 groups: FDPⅠ, DXMⅠ and FDPⅠ+ DXMⅠ); Group Ⅲ with drugs given in reperfusion phase (divided into 2 groups: FDPII and DXMⅡ). The levels of creatine kinase (CK) and troponin I (cTnI) in plasma were measured, and myocyte apoptosis index was assessed. Results: Baseline levels of CK and cTnI were similar in three groups; CK and cTnI and apoptosis index were lower or with a lower tendency in groupⅡ and in groupⅢ (P<0.05 or P<0.01); CK and cTnI showed a lower tendency in rise in FDPⅠ and DXMⅠ than in FDPII and even slower in FDP group than in DXM group; CK and cTnI levels rose slower in FDPⅠ+DXMⅠ than in FDPⅠ and DXMⅠ.CONCLUSION: FDP given during ischemia and DXM could effectively protect the myocardium from reperfusion injury following hemorrhagic shock.
, 百拇医药
PAGE：33-35
Significance of Changes in plasma lipid peroxide levels in critical lyill patients after liver transplantation
Liu Chun, Zhou Xiao-si, Geng Qiu-ming
The Third Hospital of Peking University,Beijing 100083, China; Corresponding author: LIU Chun' Tianjin Union Hospital, Tianjing 300050, China
OBJECTIVE： To investigate the relationship between plasma lipid peroxide (LPO) levels and prognosis in critically ill patients after liver transplantation. METHODS: Plasma LPO levels were measured in 18 patients within 21 days after liver transplantation, and their relations to the prognosis were analyzed. Results: Plasma LPO levels were markedly elevated in patients before state of illness got severe, especially in those with fatal outcome. The peak LPO levels were much higher in non-survivors than in survivors, and mortality rate in patients whose plasma LPO exceeding 10 μmol/L (5/6 cases) was significantly higher than those under 10 μmol/L (1/12 cases, P<0.01). The mortality rate in patients with daily fluctuation range of plasma LPO levels over 1.2 μmol·L-1·d-1 was higher than those under 1.2 μmol· L-1· d-1, and the difference was significant between two groups (P<0.01). CONCLUSION: Measurement of plasma LPO level might be of clinical significance to properly judge the critically ill conditions, and it can be used as routine examination for patients after liver transplantation.
, 百拇医药
PAGE：36-41
Study of mechanism on loss of some components from basement membrane in epithelial-interstitial junction in cutaneous pseudoepitheliomatous hyperplasia lesion
JIANG Du-yin, FU Xiao-bing, SHENG Zhi-yong, CHENG Wei, ZHOU Gang, SUN Tong-zhu Key Laboratory of Wound Repair, 304th Hospital of PLA, Beijing, 100037, China
OBJECTIVE： To investigate the relationship between the formation of pseudoepitheliomatous hyperplasia (PEH) and the mechanism of loss of some components from the basement membrane in epithelial interstitial junction (EIJ) in the treatment of cutaneous wounds, when formation of PEH lesion was induced. METHODS: Morphological change in epithelial tissue was observed with histopathologic method and electronic microscopy in 11 specimens of PEH lesions and 6 specimens of normal skin adjacent to PEH (PEH-N) from 11 patients with injured skin. The expression characteristics and distribution of pan-cytokeratin (p-CK), matrix metalloproteinase-2 (MMP-2), MMP-3, MMP-9, proliferating cell nuclear antigen (PCNA), epithelial cadherin(E-Cad) and β-catenin(β-Cat) in EMJ were detected with immunohistochemical methods. Results: Epithelial cells expressing P-CK presented squamous epithelization and extended into deep layer of mesenchyma. In epithelium-mesenchyma junction, where Ⅳ type collagen and laminin were weakly expressed, the protein contents of p-CK, E-Cad, MMP-2, MMP-3， MMP-9 were decreased, whilst the immunochemical staining of β-Cat and PCNA was apparently increased. In the junction, epithelial basal cells were observed to migrate and to depart from basal membrane; epithelial islands and isolated epithelial cells expressing p-CK in mesenchyma could be observed. Ultrastructural observation revealed deformation of epithelial basal cells, increment of nucleus/cytoplasm ratio, loosened intercellular junctions, decrement of electronic density of BM and derangement of BM stucture could be observed. CONCLUSION: Reduction in the capability of epithelial basal cells adhesion, differentiation and formation of basement membrane and cytokeratin in PEH associated with wound may be the crucial cause which controls epithelial cells migration into mesenchyma. That the contents of ColⅣ and LN were decreased may not be associated with MMPs, but with enhancement of the ratio of β-Cat/E-Cad signal might be the important mechanism of dedifferentiation of epithelial basal cells and the loss of ability of structure formation and cellular migration.
, 百拇医药
PAGE：42-44
Protective effect of propofol on liver during ischemia-reperfusion injury in patients undergoing liver surgery
Lin Li-na, Wang Wan-tie, Wu Jin-ze, Hu Zheng-yang, Xie Ke-jian
Department of Anesthesiology, First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325003, Zhejiang, hina
OBJECTIVE： To explore the protective effect of propofol on liver during hepatic ischemia/reperfusion injury (HIRI) and its mechanisms in patients undergoing liver surgery. METHODS: Eighteen patients who were scheduled for selective hepatic surgery were randomly divided into control group (n=9) and propofol treatment group (n=9). Changes of several parameters in plasma and effects of propofol on them were observed before liver ischemia, at end of ischemia and at reperfusion for 25 minutes, parameters of which included superoxide dismutase (SOD), xanthine oxidase (XO), lipid peroxide (LPO) and alanine aminotrasferase (ALT) activity, and the ultrastructure changes in liver tissue were observed under electron microscope at 25 minutes after reperfusion. Results: SOD activity decreased remarkably (P<0.01); XO activity, LPO concentration and ALT value increased significantlly (P<0.01) during HIRI, and there were abnormal changes of the hepatic ultrastructure at 25 minutes after reperfusion. Afer treatment with propofol, the variation of all parameters were alleviated markedly (P<0.05 and P<0.01). CONCLUSION: Propofol has protective effects on HIRI by reducing oxygen free radical level and inhibiting lipid peroxidation after hepatic ischemia/reperfusion in patients undergoing liver cancer surgery.
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PAGE：45-48
Influence of inhibitors of nitric oxide synthase and L-arginine on the survival rate in a rat model of traumatic shock
HUANG Zong-hai, FENG Hao-miao, HUANG Xu-liang, SUN Ying-gang, LIN Hong-wu
Department of General Surgery， Zhujiang Hospital， First Military Medical University, Guangzhou 510282, Guangdong, China
OBJECTIVE： To investigate the influence of inhibitors of nitric oxide synthase (NOS) and L-arginine on the survival rate in a rat model of traumatic shock. METHODS: A rat model of traumatic shock was established by facturing the posterior limb of the rat. Nω-nitro-L-arginine-methyl ester (L-NAME, non-selective NOS inhibitor, 10 mg/kg), amino- guanidine〔AG, selective inducible nitric oxide synthase (iNOS) inhibitor, 100 mg/kg〕 and L-arginine 〔L-Arg, the precursor of nitric oxide(NO) synthesis 100 mg/kg〕 were injected intravenously during resuscitation,survival time and survival rate were observed. Results: There were no significant changes in survival time and 24-hour survival rate between L-NAME 〔(23.80±9.09) hours and 40 percent〕 and control group〔(18.78±4.64)hours and 10 percent, both P>0.05〕; the survival time of AG group(28.72±6.25)hours and L-Arg group (30.64±8.77)hours prolonged apparently (both P<0.01), and 24-hour survival rate was also increased (both 80 percent, both P<0.01). CONCLUSION: Selective iNOS inhibitor AG and L-Arg exert benefitial effects on after traumatic shock rats.
, 百拇医药
PAGE：49-51
Effects of L-arginine on the function of platelet aggregation during hepatic ischemia/reperfusion injury
Wang Wan-tie, Lin Li-na, Pan Xue-rong, Xu Zheng-jie
Department of Pathophysiology, Wenzhou Medical College, Wenzhou 325027, Zhejiang, China
OBJECTIVE： To explore the effects of L-arginine (L-Arg) on the function of platelet aggregation during hepatic ischemia-reperfusion injury (HIRI). METHODS: The changes in maximum aggregating rate of circulating platelets (Ptmax), its maximum aggregating time (PtT) as well as its aggregating slopes (PtS) were measured. Effects of L-Arg on those parameters were observed during HIRI in 20 rabbits and 18 patients who were scheduled for elective hepatic surgery. Results: Ptmax and PtS all increased significantly (P<0.05 and P<0.01), while PtT decreased remarkably (both P<0.05) during HIRI of rabbits and patients. After treatment with L-Arg, the abnormal changes of parameters as above were all alleviated remarkably (P<0.05 and P<0.01). CONCLUSION: It is indicated that L-Arg can effectively regutate the function of platelet aggregation during HIRI.
, 百拇医药
PAGE：52-53
Effect of complex nutrients on lipid peroxidation reation in stressed rats with moist-heat combired injury
Li Ya-jie, Wang Ying, Zhai Hui-min, Xu Cai-xia, Luo Bing-de
Nanfang Hospital, First Military Medical University, Guangzhou 510515， Guangdong, China
PAGE：54-55
Change in myokinase in hypokalenice periodic paralysis and its diagnostic significance
, 百拇医药
Tu Qiu-yun, Zi Xiao-hong, Li Xiao-bo, Li Yong-hong, Chen Li-hao
The third Xiangya Medical University, Central-South University, Changsha 410013, Hunan, China
PAGE：55-56
Clinical study on charges in serum enzynce and c-reative protein levels in patients with acute poisoning
Wang Yu-jun, Lin Kai-yan, Wu Da-hong, Wang Zheng-ying, Wang Qiong, Mi Xiao-wei, Liu Zhao-hui
, 百拇医药
Qiannan People's Hospital, Qiannan 558000, Guizhou, China
PAGE：57
Significance of procalcitonin in acute pancreatitis
Pei Hong-hong, Feng Ying-qun, Yang Zheng-an, Niu Fei, Wang Ni
Xian traffic university, the second Hospital, Xian 710004, Shanxi, China
PAGE：59-62
Mitogen-activated protein kinase signaling pathway and mucosal repair after intestinal injury
, 百拇医药
Zhen Shu-yun, Fu Xiao-bing, Xu Jian-guo
304th Hospital of PLA, Beijing, 100037, China
PAGE：63-64
Guidelines of European Heart Association: Principles of Emergoncy treatment
Zhu Hai-yan, Shen Hong
General Hospital of People's Liberation Army, Beijing 100853, China, 百拇医药