c-myc反义核糖核酸抑制血管平滑肌细胞增生的实验研究(摘要)
作者：刘闺男 刘利 齐国先 曾定尹
单位：辽宁省沈阳市，中国医科大学第一临床学院 内科(110001)
关键词：
目的 目的：血管平滑肌细胞(VSMC)的增生和迁移是动脉粥样硬化形成和经皮冠状动脉腔内成形术(PTCA)术后血管再狭窄的重要病理学基础。本研究的目的是通过模仿动脉粥样硬化形成及PTCA，造成动物血管内膜损伤，局部血管内注入与生长有关的c-myc反义核糖核酸(RNA)，观察其在防止动脉粥样硬化形成及血管再狭窄中的作用，为PTCA后出现的血管再狭窄寻找新的防治途径。
方法：选健康雄性日本大白兔36只，经球囊造成股动脉内膜损伤的同时，局部给与c-myc反义RNA、正义RNA(每只1 mg)及盐水。高胆固醇(每只1.5 g/kg)喂养12周处死，取股动脉，制成4 μm石蜡切片，按S-P法进行增生细胞核抗原(PCNA)、平滑肌肌动蛋白(Actin)及内皮细胞标记分子CD34单克降抗体免疫染色。在LUZX型图像分析系统下测量内膜厚度、PCNA核阳性细胞百分数。
, http://www.100md.com
结果：盐水及正义对照组内膜厚度明显增大(371 μm)可见明显的粥样斑块灶形成，其内皮细胞也明显增生，PCNA阳性细胞数显著增多，主要分布在内膜及中膜。免疫组化染色证明，内膜内大量增生的细胞胞浆呈Actin阳性，而内皮细胞呈CD34阳性，而反义治疗组的内膜增厚仅为217 μm(P<0.01)，可见散在泡沫细胞团，PCNA阳性细胞(30.5%)明显低于对照组(63%～64%),P<0.01。
结论：c-myc反义RNA进行封闭后，达到了显著抑制VSMC增生的作用，同时明显减轻了粥样斑块病变的程度，是防止动脉粥样硬化形成及血管再狭窄的有效手段之一。
The Study on the Effect of c-myc Antisense RNA for Inhibiting the Proliferation of Vascular Smooth Muscle Cells (Abstract)
, 百拇医药
Department of Cardiology, The First Affiliated Hospital of China Medical University, Shenyang (110001), Liaoning
Liu Guinan, Liu Li, Qi Guoxian, et al.
Objective: The proliferation and migration of vascular smooth muscle cells (VSMC) are the pathological basis of atherosclerotic formation and vascular restenosis after percutaneous transluminal coronary angioplasty (PTCA). The purpose of this research was to injure the vascular intima, mimicking the formation process of atherosclerosis and PTCA and to observe the effect of c-myc antisense RNA on preventing atherosclerotic formation and vascular restenosis.
, http://www.100md.com
Methods: The femur artery intima of 36 male Japanese white rabbits was injured by balloon sac and at the same time, either c-myc antisense RNA or sense RNA (1 mg per rabbit) or saline was injected into the blood. All the rabbits were fed with hypercholesterol feed and killed in 12 weeks. The femur artery was cut into 4 μm thick paraffin sections and stained for PCNA, actin and CD34 by s-p immunohistochemistry. The thickness of the intima was measured and the percentage of PCNA positive cells was counted by image analysis (LUZX).
, 百拇医药
Results: In the control group of sense RNA and saline, the intima became much thicker (317 μm), the formation of atherosclerotic plaque was obvious and the number of PCNA positive cells in the intima and the media significantly increased. The proliferating cells in the intima were actin positive and the endothelial cells were CD34 positive. But in the antisense RNA group, the thickness of the intima is only half of that of control group (217 μm) and the number of PCNA positive cells (30.5%) were significantly lower than that of the control group (63%).
Conclusion: c-myc antisense RNA could significantly inhibit the proliferation of VSMC and attenuated the development of atherosclerotic plaque and could be used to prevent the formation of atherosclerosis and vascular restenosis., 百拇医药
单位：辽宁省沈阳市，中国医科大学第一临床学院 内科(110001)
关键词：
目的 目的：血管平滑肌细胞(VSMC)的增生和迁移是动脉粥样硬化形成和经皮冠状动脉腔内成形术(PTCA)术后血管再狭窄的重要病理学基础。本研究的目的是通过模仿动脉粥样硬化形成及PTCA，造成动物血管内膜损伤，局部血管内注入与生长有关的c-myc反义核糖核酸(RNA)，观察其在防止动脉粥样硬化形成及血管再狭窄中的作用，为PTCA后出现的血管再狭窄寻找新的防治途径。
方法：选健康雄性日本大白兔36只，经球囊造成股动脉内膜损伤的同时，局部给与c-myc反义RNA、正义RNA(每只1 mg)及盐水。高胆固醇(每只1.5 g/kg)喂养12周处死，取股动脉，制成4 μm石蜡切片，按S-P法进行增生细胞核抗原(PCNA)、平滑肌肌动蛋白(Actin)及内皮细胞标记分子CD34单克降抗体免疫染色。在LUZX型图像分析系统下测量内膜厚度、PCNA核阳性细胞百分数。
, http://www.100md.com
结果：盐水及正义对照组内膜厚度明显增大(371 μm)可见明显的粥样斑块灶形成，其内皮细胞也明显增生，PCNA阳性细胞数显著增多，主要分布在内膜及中膜。免疫组化染色证明，内膜内大量增生的细胞胞浆呈Actin阳性，而内皮细胞呈CD34阳性，而反义治疗组的内膜增厚仅为217 μm(P<0.01)，可见散在泡沫细胞团，PCNA阳性细胞(30.5%)明显低于对照组(63%～64%),P<0.01。
结论：c-myc反义RNA进行封闭后，达到了显著抑制VSMC增生的作用，同时明显减轻了粥样斑块病变的程度，是防止动脉粥样硬化形成及血管再狭窄的有效手段之一。
The Study on the Effect of c-myc Antisense RNA for Inhibiting the Proliferation of Vascular Smooth Muscle Cells (Abstract)
, 百拇医药
Department of Cardiology, The First Affiliated Hospital of China Medical University, Shenyang (110001), Liaoning
Liu Guinan, Liu Li, Qi Guoxian, et al.
Objective: The proliferation and migration of vascular smooth muscle cells (VSMC) are the pathological basis of atherosclerotic formation and vascular restenosis after percutaneous transluminal coronary angioplasty (PTCA). The purpose of this research was to injure the vascular intima, mimicking the formation process of atherosclerosis and PTCA and to observe the effect of c-myc antisense RNA on preventing atherosclerotic formation and vascular restenosis.
, http://www.100md.com
Methods: The femur artery intima of 36 male Japanese white rabbits was injured by balloon sac and at the same time, either c-myc antisense RNA or sense RNA (1 mg per rabbit) or saline was injected into the blood. All the rabbits were fed with hypercholesterol feed and killed in 12 weeks. The femur artery was cut into 4 μm thick paraffin sections and stained for PCNA, actin and CD34 by s-p immunohistochemistry. The thickness of the intima was measured and the percentage of PCNA positive cells was counted by image analysis (LUZX).
, 百拇医药
Results: In the control group of sense RNA and saline, the intima became much thicker (317 μm), the formation of atherosclerotic plaque was obvious and the number of PCNA positive cells in the intima and the media significantly increased. The proliferating cells in the intima were actin positive and the endothelial cells were CD34 positive. But in the antisense RNA group, the thickness of the intima is only half of that of control group (217 μm) and the number of PCNA positive cells (30.5%) were significantly lower than that of the control group (63%).
Conclusion: c-myc antisense RNA could significantly inhibit the proliferation of VSMC and attenuated the development of atherosclerotic plaque and could be used to prevent the formation of atherosclerosis and vascular restenosis., 百拇医药