丙酮酸乙酯对烫伤延迟复苏大鼠细胞免疫功能的影响
董月青 姚咏明 魏鹏 刘辉 董宁 于燕 盛志勇【摘要】 目的 观察丙酮酸乙酯(EP)对烫伤延迟复苏大鼠细胞免疫功能的影响，并探讨其作用机制。方法采用大鼠30%总体表面积Ⅲ度烫伤模型，103只大鼠随机分为正常对照组(n=7)、假烫伤组(n=32)、烫伤组(n=32，伤后6 h腹腔内注入40 ml/kg生理盐水)和EP(3.23 mg/ml)干预组(n=32，伤后6 h腹腔内注入40 ml/kg EP液)，分别于伤后1、3、5和7 d活杀，检测脾淋巴细胞增殖能力、白细胞介素-2(IL-2)生成及白细胞介素-2受体(IL-2R)表达。结果烫伤延迟复苏后1~7 d，脾淋巴细胞丝裂原刺激的增殖反应明显受抑制(P均<0.05)。烫伤后1~5 d IL-2生成明显减少(P均<0.05)，且烫伤后1和3 d IL-2R的表达显著降低(P均<0.05)。EP干预能够明显恢复烫伤后1~7 d脾淋巴细胞的增殖能力(P均<0.05)，伤后1、3和5 d，IL-2的生成显著增加，但对IL-2R的表达无影响(P均>0.05)。结论 EP能显著增加脾淋巴细胞的增殖能力和IL-2的产生，从而有效改善烫伤延迟复苏后细胞免疫功能障碍。
【关键词】 烧伤； 白细胞介素-2； 白细胞介素-2受体； T淋巴细胞； 丙酮酸乙酯
Effects of ethyl pyruvate on cell-mediated immune function in rats with delayed resuscitation after burn injury
DONG Yue-qing, YAO Yong-ming, WEI Peng, LIU Hui, DONG Ning, YU Yan, SHENG Zhi-yong. Burns Institute, 304 th Clinical Department of General Hospital of PLA, Beijing 100037, China
【Abstract】 Objective To investigate the effects of ethyl pyruvate (EP) on cell-mediated immune function in rats with delayed resuscitation after burn injury, and its potential regulatory mechanism. Methods Wistar rats were subjected to 30% full-thickness scald injury with delayed resuscitation. One hundred and three male rats were randomly divided into normal controls (n=7), sham scald group (n=32), scald group (n=32) in which 40 ml/kg normal saline was infused peritoneally 6 hours after scald, and EP treatment group (n=32) in which 40 mg/kg EP was injected peritoneally 6 hours after scald. Animals were sacrificed on postburn day 1, 3, 5, and 7, and spleen was collected to determine splenocyte proliferation, IL-2 production and cell-surface IL-2 receptor (IL-2R) expression. Results Splenic lymphocyte proliferation responses to T cell mitogen, concanavalin A(Con A), were depressed from 1 to 7 days after scald injury(all P<0.05). Meanwhile, in comparison with sham scald group, burn injury resulted in a significant decrease in splenic production of IL-2 on postburn day 1, 3, as well as 5, and a marked suppression of IL-2R expression on days 1 and 3 postburn (all P<0.05). Treatment with EP after burn injury showed a dramatic restoration of lymphocyte proliferation rate and increased production of IL-2 at various time points(all P<0.05). However, treatment with EP did not affect IL-2R expression compared with scalded rats(all P>0.05). Conclusion Treatment with EP could markedly elevate splenic T lymphocyte proliferation response and increase production of IL-2 following burn injury, thereby improving cell-mediated immunity in thermally injured rats with delayed resuscitation. ......
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