脉络宁注射液对肢体缺血-再灌注损伤一氧化氮及一氧化氮合酶的影

脉络宁注射液对肢体缺血-再灌注损伤一氧化氮及一氧化氮合酶的影响

http://www.100md.com 2005年2月1日中国中西医结合急救杂志2005年1月第12卷第1期

     王岱君¹，王金平¹，田华²

    (潍坊医学院①人体解剖学教研室，②保健科，山东潍坊 261042)

    【摘要】目的：探讨脉络宁注射液对肢体缺血-再灌注损伤一氧化氮(NO)和一氧化氮合酶(NOS)的影响。方法：健康成年家兔20只，随机分为对照组和实验组，建立后肢缺血-再灌注损伤动物模型。在即将恢复血流灌注时，实验组家兔自耳缘静脉注射脉络宁注射液，对照组注射等量生理盐水。在缺血前、缺血后2 h、再灌注后1 h和4 h各自从术侧股静脉采血，分离血清，测定血清NO和NOS的含量；取胫前肌行电镜观察。结果：两组血清NO含量在缺血后2 h比缺血前均明显增高(P均<0.05)，再灌注后均明显降低(P均<0.05)。两组NOS活性在缺血后2 h比缺血前明显降低(P均<0.01)，再灌注后比缺血后2 h均明显增高(P<0.05或P<0.01)，且实验组明显高于对照组(P<0.01和P<0.05)。电镜观察可见再灌注后对照组组织损伤严重，而实验组较轻。结论：脉络宁注射液能抑制肢体缺血-再灌注损伤NO的过量产生，提高NOS活性，对肢体缺血-再灌注损伤具有保护作用。

    【关键词】一氧化氮；一氧化氮合酶；脉络宁注射液；缺血-再灌注损伤，肢体

    Effect of Mailuoning injection (脉络宁注射液) on nitric oxide and nitric oxide synthase in rabbits with extremity ischemia/reperfusion injury

    WANG Dai-jun1, WANG Jin-ping1, TIAN Hua2.

    1. Department of Human Anatomy, Weifang Medical College, Weifang 261042, Shandong, China; 2. Department of Health, Weifang Medical College, Weifang 261042, Shandong, China

    【Abstract】 Objective： To investigate the effect of Mailuoning injection(脉络宁注射液) on nitric oxide (NO) and nitric oxide synthase (NOS) in rabbits with extremity ischemia/reperfusion injury. Methods： The experimental models of extremity ischemia/reperfusion injury were established in 20 healthy adult rabbits that wererandomly divided into control group and experimental group. Intravenously, normal saline was infused into rabbits of the control group and Mailuoning injection, in experimental group just before blood reperfusion. Before ischemia, 2 hours after ischemia, 1 hour after reperfusion and 4 hours after reperfusion, the samples of blood were taken from the femoral veins respectively for measurements of NO and NOS in serum. Meanwhile the tibialis anterior muscles were harvested for the ultrastructure examinations. Results： The content of NO in serum was obviously increased after ischemia and decreased after reperfusion in both two groups(both P<0.05). NOS activity was decrease significantly 2 hours after ischemia, and significantly increased after reperfusion, especially in the experimental group (P<0.05 or P<0.01). The skeletal muscle injury of the experimental group, after reperfusion, was of a mild degree than that of control group in electron microscope examinations. Conclusion： The Mailuoning injection is able to inhibit excessive production of NO induced by extremity ischemia/reperfusion, and enhance NOS activity, however, it possessesprotective effect on extremity ischemia/reperfusion injury. ......

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