北京医科大学临床肿瘤学院 北京市肿瘤防治研究所 100034

    *本课题为国家863基金资助项目(No.102-10-01-14)

    王金莹，女，1962-12-26生，河北省唐山市人，满族. 1985年华北煤炭医学院医疗系毕业，1995年北京医科大学肿瘤学硕士，内科主治医师，主要从事消化内科疾病的基础和临床研究，发表论文7篇.

    项目负责人 王金莹，北京市西城区红罗厂街1号.

    Tel: 010-66175004-14收搞日期 1996-06-26 接受日期 1997-02-26

    Alteration of p53 suppression gene in gastric mucosa and carcinogenesis

    Jin_Ying Wang, Mao_Lin Jin, You_Yong Lu and Ji_You Li

    Beijing Institute for Cancer Research, The School of Oncology, Bei jing Medical University, Beijing 100034, China

    AbstractAIM To study the alteration of p53 in a variety of gastric mucosal lesions.

    METHODS Immunohistochemical staining was used to examine the overexpression of p53 protein in 154 cases of human gastric mucosal lesions from endoscopic biopsies. We also used PCR-SSCP analysis and DNA sequence to detect p53 exon 5 to 8 mutation.

    RESULTS Positive nuclear staining for p53 protein was 33.3%(10/30 cases) in gastric carcinoma except non-malignant mucosal lesions, and p53 mutation of exon 5 to 8 was determined in 6.7% (1/15 cases) of intestinal metaplasia, 20% (3/15 cases) of dysplasia, and 54.5% (6/11 cases) of gastric carcinoma. We confirmed p53 misense mutation (G→T transversion) and delete C of exon 5 in gastric carcinoma and misense mutation (G→C transversion) of exon 6 in dysplasia by using DNA sequence analysis. ......