全反式维甲酸诱导人胃癌细胞株SGC-7901分化的研究
维甲酸,类似物和衍生物胃肿瘤,药物疗法肿瘤细胞，培养的,药物作用,项目负责人,All,-trans-retinoicacidinduceddifferentiationinhumangastriccarcinomacelllineSGC-,7901,Abstract,AIM,METHODS,RESULTS,CONCLUSION,Subjectheadin
第四军医大学唐都医院消化内科 陕西省西安市 710038陈宇，女，1970-09-11生，重庆市人，汉族.1994年第四军医大学医疗系毕业，现为唐都医院消化内科硕士研究生.
项目负责人 陈宇,陕西省西安市四医大唐都医院消化内科(710038).
Tel: 029-3524578-77121.收搞日期 1997-01-21 接受日期 1997-02-26
All-trans-retinoic acid induced differentiation in human gastric carcinoma cell line SGC-7901
Yu Chen and Cai-Fu Xu
Department of Gastroenterology, Tangdu Hospital, The Fourth Military Medical University, Xi′an 710038, Shaanxi Province, China
Abstract
AIM To study the effect of alltransretinoic acid (ATRA) on human gastric carcinoma cell line SGC-7901.
METHODS At the base of cell culture in vitro, the effect of dif ferentiationinducing activity of ATRA was observed in cytomorphology, cell m ultiplication kinetics, expression of oncogene and tumorigenesis in nude mice.
RESULTS ATRA of 1μmol/L can inhibit the growth of SGC-7901 (P<0.01) and its maximum inhibition rate is 52.73% with no sign of toxicity for cells. Morphological changes of induced cells were observed. Cell cycle analysis by flowcytometry showed that percentage of G1 increased. Cells have been treated with ATRA at dose of 1μmol/L for two weeks almost lost their abilities to form clones in soft agar and its carcinoma relative antigen MGb2 decreased by 58.8%. Moreover, as determined by dot blot, the expression of two kinds of oncogenes c-myc and c-ki-ras mRNA were significantly decreased and suppressor gene wtp53 mRNA were increased. The animal model was human gastric carcinoma SGC-7901 xen ografed to BALB/C nude mice. The ability of metastatic carcinoma formation of treated cells was weakened. ......
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