采用改进的差示PCR技术分离胃癌差异表达基因的研究
胃肿瘤,诊断;RNA,信使;聚合酶链反应;基因表达;基因诊断,项目负责人,Correspondenceto,DifferentiallyexpressedgeneswereisolatedingastriccarcinomabyoptimiseddifferentialdisplayPCR,Abstract,,AIM,METHODS,RESULT
第四军医大学全军基因诊断技术应用研究所 陕西省西安市 710033崔大祥，男，1966-02-03生，安徽省桐城县人，汉族. 1990年第二军医大学毕业，1995年第四军医大学肿瘤学硕士，1998年第四军医大学分子生物学博士，主要从事肿瘤研究，承当国家自然科学基金课题(No.39470683)一项，发表论文20篇.
项目负责人 崔大祥，710033，第四军医大学全军基因诊断技术应用研究所，陕西省西安市长乐西路17号.
Correspondence to Dr. Da-Xiang Cui, Chinese PLA Institute of Gene Diagnosis, 17 Changle Xilu, Xi'an 710033,Shaanxi Province, China
Tel. +86·29·3285729E-mail. Cuidx@igd. edu. cn
收稿日期 1998-07-15
Differentially expressed genes wereisolated in gastric carcinoma by optimised differential display PCR
Da-Xiang Cui, Xiao-Jun Yan and Cheng-Zhi Su
Chinese PLA Institute of Gene Diagnosis of the Fourth Military Medical University, Xi'an 710033, Shaanxi Province, China
Abstract
AIM To establish optimised condition of mRNA DD-PCR and to isolate messenger RNAs differentially expressed in cell line GC7901 or GES-1; to establish gene diagnosis method for gastric cancer.
METHODS By using cell line GC7901 and GES-1 as research targets, the optimised condition of mRNA DD-PCR was explored. By optimised condition, cDNA fragments differentially expressed in GC7901 or GES-1 were isolated. Among these fragments 17 cDNA fragments were hybridized to total RNA of cell line GC7901 and GES-1, and sequenced as well as GenBank similar examination. By using obtained sequences, 17 pairs of primers were designed, and these primers were used to examine clinical samples. ......
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