有效原代猪肝细胞培养体系的研制
人工肝;肝细胞;细胞,培养的,*国家自然基金资助项目，No.39570212，广东省“五个一”重点课题.,项目负责人,Correspondenceto,Aneffectivesystemforculturingprimaryporcinehepatocytes*,,Abstract,AIM,METHODS,RESULTS,CONCLUSION,Subj
第一军医大学附属珠江医院普通外科 广东省广州市 510282*国家自然基金资助项目，No.39570212，广东省“五个一”重点课题.
项目负责人 陈柯，510282，第一军医大学附属珠江医院普通外科，广东省广州市
Correspondence to Ke Chen, Department of General Surgery, Zhujiang Hospital, First Military Medical College
Guangzhou 510282, Guangdong Province, China
收稿日期 1998-06-06 接收日期 1998-12-12
An effective system for culturing primary porcine hepatocytes*
Ke Chen, Yi Gao, Yu-Xian Pan and Ji-Zhen Yang
Department of General Surgery, Zhujiang Hospital, First Military Medical College, Guangzhou 510282, Guangdong Province, China
Abstract
AIM To establish an effective primary porcine hepatocytes culturing system for the biological material of BAL.
METHODS Porcine hepatocytes were harvested by two-step perfusion collagenase method. Individually, cells were plated at a concentration of 3×105 in serum-free DMEM culture medium, DMEM culture supplemented with 10% fetal calf serum or 100mL/L porcine portal vein serum, changes in cell spreading with time in culture was determined by taking phase contrast micrographs of cells. The concentration of albumin and urea were measured. ......
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