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编号:10696901
胆囊结石患者胆汁、粘膜和结石需氧菌和厌氧菌脱氧核糖核酸研究
http://www.100md.com 2000年1月15日 《世界华人消化杂志》 2000年第1期
胆结石;DNA;细菌;胆汁,项目负责人,Correspondenceto,AstudyonDNAofaerobicandanaerobicbacteriainbile,mucosaandstoneingallstonepatients,,Abstract,AIM,METHODS,RESULTS,CONCLUSION,Subjectheadings,摘要
     中国人民解放军第一军医大学珠江医院肝胆外科 广东省广州市 510282

    方驰华,男,1958-06-20生,湖北省汉川市人,1981年毕业于第四军医大学附属空军医学院,1994年同济医科大学肝胆外科硕士,副主任医师. 主攻胆道外科尤其梗阻性黄疸基础、临床及手术、腹腔镜外科和重症急性胰腺炎. 发表学术论文265篇,参加编著2部,获全军科技奖15项.

    项目负责人
方驰华,510282,广东省广州市工业大道中253号,中国人民解放军第一军医大学珠江医院肝胆外科.

    Correspondence to Chi-Hua Fang, Department of Hepatobiliary Surgery, Zhujiang Hospital, 253 Gongye Avenue, Guangzhou 510282, Guangdong Province, China

    Tel. +86-20-85143557

    收稿日期 1999-06-30 接收日期 1999-11-20

    A study on DNA of aerobic and anaerobic bacteria in bile, mucosa and stone in gallstone patients

    Chi-Hua Fang

    Department of Hepatobiliary Surgery, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, Guangdong Province, China

    

    Abstract


    AIM To study bacteria deoxyribonucleic acid (DNA) in bile, mucosa and stone, and provide evidence of molecular biology of bacteria in gallstones formation.

    METHODS DNA of five aerobic bacteria including Bacillus Coli, Kelbsiella Pneumonie, Bacillus Typhoid, Helicobacter pylori, Bacillus Proteus and four anaerobic bacteria including Bacteroides Fragilis, Peptococcus, Clostridium and Bacillus Perfringens in previous samples was tested with polymerase chain reaction (PCR) in 70 gallstone patients. ......

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