幽门螺杆菌vac A基因毒性相关片段的克隆及序列分析
幽门螺杆菌;vacA基因;聚合酶链反应;克隆;序列分析,幽门螺杆菌vacA基因毒性相关片段的克隆及序列分析,项目负责人,Correspondenceto,CloningandsequenceanalysisofafragmentrelatedtovacuolatingcytotoxinofvacAofHelicobacterpylori,Abstract,AIM,METHODS
中国人民解放军第四军医大学基因诊断技术研究所 陕西省西安市 710032江红，女，1967-11-06生，辽宁省北票市人，汉族. 1989年陕西师范大学生物系本科毕业，1992年西南师范大学生物化学专业硕士毕业，现攻读第四军医大学分子生物学与生物化学专业博士学位，主要从事基因克隆与表达的研究. 发表论文5篇.
项目负责人 江红,710032,陕西省西安市,中国人民解放军第四军医大学基因诊断技术研究所.
Institute of Genetic Diagnosis, Fourth Military Medical University, Xi＇an 710038, Shaanxi Province, China
Correspondence to Hong Jiang, Institute of Genetic Diagnosis, Fourth Military Medical University, No 17 Changle Xilu, Xi＇an 710038, Shaanxi Province, China
Tel. 0086-29-3374771, Fax. 0086-29-3216587
Email. jhmqf@263.net
收稿日期 2000-04-03 接收日期 2000-04-13
Cloning and sequence analysis of a fragment related to vacuolating cytotoxin of vacA of Helicobacter pylori
Hong Jiang, Xiao-Jun Yan, Cheng-Zhi Su, Feng-Chan Han, Yong-Qiang Feng and Yu Hou
Abstract
AIM We clone and sequence a fragment related to vacuolating cytotoxin of vacA from Helicobacter pylori (Hp) strain 88022 for the clinical analysis of Hp vacA.
METHODS A 903bp fragment was amplified by the polymerase chain reaction. The template DNA was purified from Hp strain 88022. We designed the primers located in 316bp-335bp and 1198bp-1218bp respectively, from the relatively reserved region of vacA gene. DNA fragment containing the sequence codes for amino acids 34-334, was digested by BamHⅠ and PstⅠ and cloned into pGEM-3Zf(-) plasmid. Its sequence was determined with an autosequencing instrument from two directions and compared with those having the corresponding region of vacA from other Hp strains. ......
您现在查看是摘要页，全文长 25716 字符。