肝细胞癌组织中HCV基因型的逆转录原位PCR检测
肝肿瘤;癌，肝细胞;丙型肝炎病毒;病理学，临床;聚合酶链反应;基因型;原位杂交;血清学,项目负责人,Correspondenceto,HCVgenotypesexpressioninhepatocellularcarcinomabyreversetranscription,insitupolymerasechainreacti
上海医科大学病理教研室 上海市 200032苏英豪，男，1962-08-22生，安徽省凤台县人，汉族. 1984年上海医科大学医疗系本科毕业，1990年安徽医科大学病理学硕士毕业，副教授，1997年考入上海医科大学攻读病理学博士学位，主要从事肝癌分子病理学研究，发表论文13篇.
项目负责人 朱世能，200032，上海市，上海医科大学病理教研室.
Department of Pathology, School of Basic Medical Sciences, Shanghai Medical University, Shanghai 200032, China
Correspondence to Ying-Hao Su, Department of Pathology, School of Basic Medical Sciences, Shanghai Medical University, Shanghai 200032, China
Tel. 0086-21-64041900 Ext.2532
Email. suyh1019@sohu.com
收稿日期 2000-01-14 接收日期 2000-03-01
HCV genotypes expression in hepatocellular carcinoma by reverse transcription in situ polymerase chain reaction
Ying-Hao Su, Shi-Neng Zhu, Shi-Lun Lu and Ying-Hong Gu
Abstract
AIM To study the relationship between hepatitis C virus (HCV) genotypes and development of hepatocellular carcinoma (HCC) at molecular pathologic level.
METHODS Reverse transcription in situ polymerase chain reaction (RT-IS PCR) method was performed to detect genotypes 1b and 2a of HCV in HCC tissue and noncancerous area of 86 cases. HCV genotypes were simultaneously checked by Okamoto＇s method using RNA extracts from HCC tissues. ......
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