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雌激素以及胆酸负荷和胆汁流失对大鼠肝胆功能的影响
http://www.100md.com 2000年9月15日 《世界华人消化杂志》 2000年第9期
雌激素;胆酸;妊娠期肝内胆汁瘀积症;胆汁;肝胆功能;大鼠,项目负责人,Correspondenceto,Effectofestrogen,cholicacidloadingandbiledrainingonhepatobiliaryfunctionsinrats,,Abstract,AIM,METHODS,-1,RESULTS,CON
     南京农业大学动物医学院动物生理生化农业部重点开放实验室 江苏省南京市 210095

    李新平,南京农业大学1999毕业博士研究生,现在重庆大学生物工程学院从事博士后研究.

    项目负责人
毛鑫智,教授、博士生导师,江苏省南京市玄武区卫岗1号,南京农业大学动物医学院动物生理生化农业部重点开放实验室.

    Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing Agricultural University, College of Veterinary Medicine, Nanjing 210095, Jiangsu Province, China

    Correspondence to
Prof. Xin-Zhi Mao, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095,Jiangsu Province, China

    Tel. 0086-25-4396264, Fax. 0086-25-4442173

    Email.xzml@pub.jlonline.com

    收稿日期 2000-04-18 接收日期 2000-06-10

    Effect of estrogen, cholic acid loading and bile draining on hepatobiliary functions in rats

    Xin-Ping Li and Xin-Zhi Mao

    

    Abstract


    AIM To study the effects of estrogen, cholic acid loading and bile draining on hepatobiliary functions in order to clarify the cause and pathogenesis of intrahepatic cholestasis of pregnancy (ICP) in women.

    METHODS Thirty-seven Wistar female rats with body mass of 170g-200g were randomly divided into three groups. Estrogen group (E, 10), cholate group (CA, 17) and control group (C, 10). Rats in group E were injected with benzogynestryl (5mg·kg-1) once a day in 5 days and in group C were done the same as group E in addition to injection of benzogynestryl substituted by propylene glycol as well as the rats in group CA were infused with cholate saline solution (2.4mg·kg-1, 0.2% w/v) 3 times interval 20min before the experiment. Blood samples were taken from infraorbitalis V. Plexus at the beginning of experiment and then catheter was put into bile duct for collecting the bile every 15min in the first hour and total amount in second hour. After 2h, the blood samples were also taken before experiment. Estrodiol (E2), GCA and AST, ALT, GGT, ALP,CHOL, TBA and TBIL in plasma and bile were measured with RIAs and autobioanalyzer by enzymetic method, respectively. ......

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