人环氧合酶-2(hCOX-2)编码基因的克隆及其 反义核酸转染胃癌细胞的初步研究
环氧合酶;反转录PCR;反义RNA;肿瘤;基因转染,人环氧合酶-2(hCOX-2)编码基因的克隆及其反义核酸转染胃癌细胞的初步研究,国家自然科学基金资助项目，,No.39870320,项目负责人,SupportedbytheNationalNaturalScienceFoundationofChina,No.39870320,Correspondenceto,Clon
中国人民解放军第四军医大学西京医院消化病研究所 陕西省西安市 710033吴汉平，男，1970-06-25生，辽宁省大连市人，汉族. 1994年第二军医大学医疗系本科毕业，现为第四军医大学西京医院全军消化病研究所硕士研究生. 主要从事消化道肿瘤研究.
国家自然科学基金资助项目，No.39870320
项目负责人 吴开春，710033，陕西省西安市，中国人民解放军第四军医大学西京医院消化内科.
Department of Gastroenterology, Xijing Hospital, Fourth Military Medical University, Xi＇an 710033, China
Supported by the National Natural Science Foundation of China, No.39870320
Correspondence to Kai-Chun Wu, Department of Gastroenterology, Xijing Hospital, Fourth Military Medical University,Xi＇an 710033, Shaanxi Province, China
Tel. 0086-29-3375230
Email. kaicwu@pub.xa_online.sn.cn
Received 2000-07-26 Accepted 2000-09-18
Cloning of human cyclooxygenase-2 (COX-2) encoding gene and study of gastric cancer cell transfected
with its antisense vector
Han-Ping Wu, Kai-Chun Wu, Ling Li, Li-Ping Yao, Mei Lan, Xin Wang and Dai Ming Fan
Abstract
AIM To obtain human COX-2 encoding gene, construct its sense, antisense eukaryotic vectors and transfect its antisense vector into COX-2 highly expressing gastric cancer cell line in order to further study its biological activities and role in tumorigenesis. ......
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