全反式视黄酸对人α1(Ⅰ)前胶原启动子活性的作用
胶原,遗传学;启动区(遗传学);维甲酸,药理学;胰岛素样生长因子Ⅰ,药理学;胰岛素,药理学,国家自然科学基金资助项目,No.39870301,项目负责人,ProjectsupportedbyNationalNaturalScienceFoundationofChina,No.39870301,Corre
中国人民解放军第二军医大学长征医院实验诊断科 上海市 200003伍严安，女，1954-05-24生，福建省上杭县人，汉族. 医学博士，主要从事肝纤维化机制的研究.
国家自然科学基金资助项目, No.39870301
项目负责人 伍严安，350001，福建省福州市东街134号，福建省立医院检验科.
Department of Laboratory Medicine, Changzheng Hospital,Second Military Medical University, Shanghai 200003, China
Project supported by National Natural Science Foundation of China, No.39870301
Correspondence to Yan-An Wu, Department of Laboratory Medicine, Fujian Provincial Hospital, 134 Dongjie Street, Fuzhou 350001, Fujian Province,China
Tel. 0086-591-7557768 Ext.3012
Email. yananwu@pub6.fz.fj.cn
Received 2000-09-01 Accepted 2000-09-18
Effect of all-trans-retinoic acid on promoter activity of human α1(Ⅰ) procollagen gene
Yan-An Wu, Chun-Fang Gao, Wei-Dong Wan, Hao Wang and Xian-Tao Kong
Abstract
AIM To study the effect of all-trans-retinoic acid (t-RA) on promoter activity of human α1(Ⅰ) procollagen gene and the influence of insulin-like growth factor 1 and insulin on the effect.
METHODS Human skin fibroblasts were transiently transfected with the construct of pCOLH2.5 containing -2483-+42bp of the procollagen gene and CAT as reporter gene. The cells subsequently treated with t-RA or/and IGF-1 or insulin. The CAT activity was assessed 24h after t-RA and the cytokines added. ......
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