中国人民解放军第302医院传染病研究所基因治疗研究中心 北 京市 100039

    国家自然科学基金资助项目 No.39970674

    李克，男，1965-12-26生，河北宁晋人.1986年华山冶金医专医疗系毕业，19 94年军医进修学院硕士毕业，目前是军医进修学院在读博士研究生.主治医师，主要从事肝 脏疾病的临床及研究工作.

    项目负责人 李克，解放军第302医院传染病研究所基因治疗研究中 心. lisahib@yahoo.com

    电话：010-66933392

    收稿日期 2001-08-24 接受日期 2001-09-16

    Screening and Cloning of gene of hepatocyte protein 1 interacti ng with HCV core protein

    Ke Li， Lin Wang， Jun Cheng， Ling-Xia Zhang， Hui-Juan Duan， Yin- Ying Lu， Ji-Zhen Yang，Yan Liu， Yuan Hong， Xiao-Bing Xia， Gang Wang， Ji ng Dong， Li Li， Yan-Wei Zhong and Ju-Mei Chen

    Gene Therapy Research Center, The 302 Hospital of PLA,Feng Tai Road 26, 100039, Beijing, China.

    Supported by National Natural Science Foundation, No.39970674

    Correspondence to: Ke Li，The 302 Hospital of PLA, FengTai Roa d 26, 100039, Beijing, China.

    Received 2001-08-24 Accepted 2001-09-16

    Abstract

    AIM To clone the unknown gene of hepatocyte protein interact ing with hepatitis C virus core protein.

    METHODS Using the yeast-two hybrid system 3, we constructed ba it plasmid of hepatitis C virus core. After it was proved that hepatitis C virus core protein could firmly expressed in AH109 yeast strain, we performed yeast- two hybrid by mating AH109 with Y187 that transformed with liver cDNA library pl asmids pACT2 and then plated on quadrople dropout (QDO) medium and assayed for X -α-gal activity. The genes from yeast colonies could grow on QDO and had α- gal activity. We also divided the gene of HCV core into 3 parts,core(L), 2/3 c ore(M) and 1/3 core(S) fragments, three fragments was crossed back with the gene we had cloned. ......