当前位置: 首页 > 期刊 > 《世界华人消化杂志》 > 2002年第7期
编号:10693791
诱导HSP70减轻肠上皮细胞缺氧/再给氧的损伤
http://www.100md.com 2002年7月15日 《世界华人消化杂志》 2002年第7期
EffectofinducibleHSP70onhypoxia,reoxygenationinducedinjuryofintestinalepithelialcellinvitro,Abstract,摘要,目的,方法,结果,结论,0,引言,1材料和方法,1.2,1.3.1MTT,检测细胞活力,1.3.2HSP70,免疫组化检测,1.3.3IEC-6,细胞凋亡的检
     袁志强,李晓鲁,彭毅志,中国人民解放军第三军医大学西南医院烧伤研究所 重庆市 400038

    袁志强,男,1976-3-18生,湖南省汝城县人,汉族.1997年第三军医大学毕业,现为第三军医大学烧伤外科学博士研究生,主要从事脏器缺血再灌注损伤及感染防治的研究,发表论文8篇.

    国家重点基础研究发展规划项目,(G1999054202)

    项目负责人 彭毅志,400038,重庆市高滩岩,第三军医大学西南医院烧伤研究所. cqmed@sina.com

    电话: 023-68754175

    收稿日期 2002-03-05 接受日期 2002-03-22

    Effect of inducible HSP70 on hypoxia/ reoxygenation induced injury of intestinal epithelial cell in vitro

    Zhi-Qiang Yuan, Xiao-Lu Li, Yi-Zhi Peng

    Zhi-Qiang Yuan, Xiao-Lu Li, Yi-Zhi Peng, Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing 400038, China

    Supported by major stat basic Research development program of China (G1999054202)

    Correspondence to: Dr Yi-Zhi Peng, Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing 400038, China. cqmed@sina.com

    Received 2002-03-05 Accepted 2002-03-22

    AbstractAIM: To study the mechanism and effect of inducible heat shock protein 70 (HSP70) on hypoxia/reoxygenation induced injury of intestinal epithelial cell.

    METHODS: Cultured IEC-6 cells were divided into three groups: Control group (C), hypoxia/reoxygenation group (H/R) and heat shock pretreated group (HS+H/R). After 1 hour of hypoxia followed by 1 hour of reoxygenation, Immunohistochemical staining was used to determine the expression of HSP70 in IEC-6 cells. Cell viability was measured by MTT assay and lactate dehydrogenase leakage were analyzed, and the cell apoptosis was evaluated with TUNEL staining. ......

您现在查看是摘要页,全文长 18483 字符