宋刘来,罗和生,余保平,武汉大学人民医院消化内科 湖北省武汉市 430060

    宋刘来,男,1972-10-22生,安徽省安庆市人,汉族.1996年安徽蚌埠医学院本科毕业,现为武汉大学人民医院硕士研究生,研究方向为消化系疾病的诊治.

    项目负责人:罗和生,430060,湖北省武汉市,武汉大学人民医院消化内科. luotang@public.wh.hb.cn

    收稿日期:2002-07-12 接受日期:2002-07-29

    Effects of hepatocyte growth factor on fibrosis and hepatic expression of MMP-1 andTIMP-1

    Liu-Lai Song,He-Sheng Luo,Bao-Ping Yu

    Liu-Lai Song, He-Sheng Luo,Bao-Ping Yu,The Devision of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China

    Correspondence to: He-Sheng Luo,The Devision of gastroenterology,Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China. luotang@public.wh.hb.cn

    Received:2002-07-12 Accepted:2002-07-29

    AbstractAIM:To investigate the effect of hepatocyte growth factor (HGF) on severity of liver fibrosis and hepatic expressions of MMP-1,TIMP-1 and to explore the mechanism of HGF in preventing liver fibrosis in rats.

    METHODS:Eighty male Wistar rats were randomly divided into normal control group (Group A, 16 rats), liver fibrosis model group (Group B, 54 rats) and HGF therapy group (Group C, 10 rats). The liver fibrosis model was induced by administration CCl4 intraperitoneally.Rats in Group C had been administered HGF for six weeks and were sacrificed afterwards. Eight rats from each of group A and B were randomly sacrificed on week 6 simultaneously as that in group C. The remaining rats in-group B were randomly further subdivided into liver fibrosis model group (Group D, 12 rats) and HGF therapy group (Group E, 10 rats),HGF was administered to rats in group E on week.7.All rats in group D and E were sacrificed on week 10. Liver function and levels of serum hyaluronic acid (HA), mucin (LN), collegen type IV (CIV), procollagen III (PCIII) were tested; the expression of MMP-1 and TIMP-1 were determined by immunohistochemical staining and analyzed by computer. ......