潘勤,李定国, 汪余勤,徐芹芳,上海第二医科大学附属新华医院消化内科 上海市 200092

    潘勤,男, 1971-03-12生,上海市人, 汉族. 2003年获上海第二医科大学博士学位.主要研究方向为肝纤维化的发生机制及药物防治.

    上海市科委基金资助项目,No. 004119047

    项目负责人:潘勤, 200092, 上海市控江路1665号,上海第二医科大学附属新华医院. fangchunhua@online.sh.cn

    电话:021-65790000-5319 传真:021-55571294

    收稿日期:2003-12-29 接受日期:2004-02-24

    Establishment and identification of a novel immortalized rat hepaticstellate cell line HSC-PQ

    Qin Pan, Ding-Guo Li, Yue-Qin Wang, Qin-Fang Xue

    Qin Pan, Ding-Guo Li, Yue-Qin Wang, Qin-Fang Xue, Department ofGastroenterology, Xinhua Hospital, Shanghai Second Medical University,Shanghai 200092, China

    Correspondence to: Qin Pan, Department of Gastroenterology, XinhuaHospital, 1665 Kongjiang Road, Shanghai 200092, China. fangchunhua@online.sh.cn

    Received: 2003-12-29 Accepted:2004-02-24

    AbstractAIM: To establish and identify a novel immortalized rat hepaticstellate cell (HSC) line.

    METHODS: Primary HSCs were isolated from the liver of adult male Sprague-Dawleyrats by a combination of pronase-collagenase perfusion and densitygradient centrifugation. Then a new HSC line, being HSC-PQ, wasestablished, cultured, and passaged by way of cellular clone. Furthermore,cellular dynamics, light microscopy, transmission electron microscopy, andimmunocytochemistry were employed to investigate characteristics of theHSC line. ......