人类抗凋亡基因survivin的克隆及其原核表达
杨欣艳,王孟薇,王刚石,尤纬缔,项目负责人,:,Cloningandexpressionofhuman,anti-apoptosisgenesurvivininEscherchiacoli,Correspondenceto:,Abstract,摘要,目的,方法,结果,结论,0,引言,1,材料和方法,1.1材料,1.2,方法,2,结果,2.1RNA
杨欣艳,北京军区总医院消化内科 北京市 100700王孟薇,王刚石, 尤纬缔,中国人民解放军总医院西院消化科北京市 100853
项目负责人:杨欣艳,100700, 北京市,北京军区总医院消化内科.
电话:023-68774905
收稿日期:2002-03-19 接受日期:2002-05-11
Cloning and expression of human anti-apoptosisgene survivin inEscherchia coli
Xin-YanYang, Meng-Wei Wang, Gang-Shi Wang, Wei-Di You
Xin-Yan Yang, Department of Gastroenterology, Beijing Military GeneralHospital, Beijing 100700, China
Meng-Wei Wang, Gang-Shi Wang, Wei-Di You, Department of Gastroenterology,PLA General Hospital, Beijing 100853, China
Correspondence to: Xin-Yan Yang, Department of Gastroenterology,Beijing Military General Hospital, Beijing 100700, China.
Received: 2002-03-19 Accepted:2002-05-11
AbstractAIM: To clone and express human antiapoptosis gene-survivin (SVV) inEscherchia coli.
METHODS: The SVV cDNA was obtained by using RT-PCR method with total RNAextracted from the human gastric cancer cell line, SGC7901. Then it wascloned into the pGEM-T easy vector, and subcloned into expression vectorpRSET. After proved to be correct by sequencing, recombinant expressionplasmid pRSET-SVV was transformed into E.coli BL21 (DE3). Thefusion protein was produced by IPTG induction. ......
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