【摘要】 目的 观察藻酸双酯钠(PSS)对豚鼠心室肌细胞离子流的影响。方法 采用Ⅰ型胶原酶分离单个心室肌细胞的方法，利用全细胞膜片箝记录技术，在不同箝制条件下，记录并分析了PSS对L-型Ca 2+ 电流(I Ca，L )、内向整流K + 电流(I K1 )的影响。结果 (1)PSS使I Ca，L 的激活时间常数变大，电流幅度减小，I-V曲线上移。(2)PSS可使I K1 的内向成分和外向成分均增加，PSS能使I K1 的激活时间常数减小，同时，I K1 的电导值变大。结论 PSS对L-型Ca 2+ 电流有抑制作用，对内向整流K + 电流有加强作用。

    关键词 藻酸双酯钠 心室肌细胞 膜片箝技术 L-型Ca 2+ 电流 内向整流K + 电流

    Han Shengna，Cui Jinjuan，Zhang Zhao，et al.

    Department of Physiology，Medical College of Zhengzhou University，Zhengzhou450052.

    【Abstract】 Objective To examine the effects of polysaccharide sulfate(PSS)on ionic currents in guinea pig ventricular myocytes.Methods Single ventricular cells of guinea pigs were isolated with a collagenase(I-type)disˉpersion method，using whole-cell recording method to record L-type Ca 2+ current(I Ca，L )and the inward rectifier K + current(I K1 ).Results (1)PSS increased the time constant of activation and decreased the current amplitude of I Ca，L .(2)PSS increased not only the inward component but also the outward component of the peak I K1 .PSS decreased the time constant of activiation of I K1 and PSS increased the maximal slope conductance of I K1 .Conclusion PSS deˉcreases L-type Ca 2+ current ......