【摘要】 目的 探讨金克-槐耳颗粒是否能逆转人乳腺癌MCF-7/A细胞对阿霉素(ADM)的多药耐药性(MDR)及其逆转机制。方法 采用四甲基偶氮唑蓝(MTT)法测定药物的细胞毒性、耐药倍数和逆转倍数;应用荧光分裂光光度计测定细胞内ADM药物浓度;荧光定量PCR仪测定药物对细胞内MDR-1mRNA表达水平的影响。结果 (1)0.01mg/ml剂量的槐耳颗粒能显著降低化疗药物ADM对人乳腺癌耐药细胞株MCF-7/A的IC 50 (3.95μg/ml)，与逆转前耐药细胞株MCF-7/A的IC 50 (17.6μg/ml)相比差异有显著性(P<0.05)，其逆转倍数为4.5倍，耐药倍数为21.7倍。(2)0.01mg/ml槐耳颗粒能显著增加耐药细胞内ADM浓度，实验组与对照组比较差异有非常显著性(P<0.01)。(3)人乳腺癌敏感细胞株MCF-7/S无MDR-1mRNA表达，低毒剂量和非细胞毒性剂量的槐耳颗粒使MCF-7/A细胞的耐药基因MDR-1表达水平下调，与对照组相比差异有非常显著性(P<0.01)。结论 研究表明金克-槐耳颗粒具有逆转MCF-7/A细胞耐药性的作用，逆转机制是使耐药基因MDR-1表达水平下调，证明金克具有双效性，一是其抗肿瘤活性，二是一种耐药逆转剂。

    关键词 乳腺癌 多药耐药性(MDR) MCF-7/A 金克 逆转作用

    Zhang Yubao，Ma Yuyan，Wang Bo，et al.

    The surgical department of the tumor hospital of harbin medical university，150040.

    【Abstract】 Objective The aim of this study is to discuss whether the Jinke plaster can reverse the MDR of the breast cancer cell strian MCF-7/A to ADM or not and the mechanism.Methods MTT method was used to deˉtect the cell toxicity，drug-resistant multiple and reversing multiple;Fluorospectorphotometer was used to detect the concentration of ADM in cell;Fluoro-quantitative PCR method was used to detect the effect of the drug on the exˉpressing level of MDR-1mRNA in cell.Results (1)0.01mg/ml H uaierkeli plaster can significantly reduce the IC 50 (3.95μg/ml)of ADM to the drug resistant cell strain(MCF-7/A)of breast cancer.It has significant difference comparing to the IC 50 (17.67μg/ml)the drug-resistant cell strain MCF-7/A without reverseing(P<0.05) ......