【摘要】 目的 探讨大鼠急性脊髓损伤(SCI)后不同时段髓内诱生型一氧化氮合酶(iNOS)mRNA动态表达。方法 36只SD大鼠随机分组，任选一组作为对照。采用Allen法制作急性脊髓损伤模型，在损伤后2，6，12，24，48h等时段，以反转录酶聚合酶链式反应(RT-PCR)分析iNOS mRNA表达。在SCI后24h，用免疫组化方法分析iNOS在脊髓不同类型神经细胞中的表达与分布。结果 脊髓损伤后，神经元、星形细胞和小胶质细胞中均可见iNOS表达，以神经元表达为主。SCI后2h可见iNOS表达0.56±0.02，24h达高峰1.20±0.05，48h开始降低0.70±0.06。结论 研究资料提示SCI后脊髓内iNOS表达增高，过量产生的NO加重了继发性脊髓损伤。

    关键词 脊髓损伤 一氧化氮 一氧化氮合酶 基因表达

    Tang Jian，Shi Hongguang，Zhao Jian

    Department of Orthopaedics，Haimen People’s Hospital.Jiangsu226001.

    【Abstract】 Objective To explore the expression of induced nitric oxide synthase(iNOS)mRNA in spinal cords after acute spinal cord injured(SCI)in SD rats.Methods Expression of iNOS-mRNA in injured spinal cords was detected by RT-PCR after the2nd，6th，12th，24th，48th h after injured with Allen’s weight-dropping model.The distribution of iNOS was examined by immunohistochemistry in tissues of spinal.Results The iNOS expression was observed first at2h，peaked at24h ......