【摘要】 目的 研究热休克诱导肝癌HepG2细胞HSP70的表达规律及槲皮素对HSP70表达的抑制效应，为中医中药治疗肝癌和提高肝癌热疗效果提供了理论依据。方法 (1)体外培养肝癌HepG2细胞，恒温水浴42℃热休克HepG2细胞90min。(2)采用免疫细胞化学法、流式细胞术检测热休克前、热休克后2h、4h、8h、12h、24h时HSP70的表达规律;热休克前1h应用槲皮素对HSP70的抑制效应;热体克后应用槲皮素对HSP70的抑制效应。结果 (1)42℃热休克诱导HepG2细胞，HSP70表达增多，阳性细胞数升高，4h达到最高(11.47%)，8h后回落到低值。(2)热休克前应用槲皮素能有效抑制热休克诱导HSP70的过量表达。(3)热休克后加入槲皮素，对已诱导产生的HSP70无明显抑制作用。结论 热休克能诱导肝癌HepG2细胞的HSP70过量表达，热休克前应用槲皮素能抑制人肝癌HepG2细胞HSP70的过量表达，提示桷皮素可作为一种热疗的增敏剂。

    关键词 槲皮素 HepG2细胞 热疗 热休克蛋白70

    A study of the inhibition effect of Quercetin on HSP70of HepG2cells induced by heat shock Zeng Wenting，Tang Wubing，Ma Peiqiu，et al. Department of Hepatology Research，First Affilliated

    Hospital of Guangzhou Medical College，Guangzhou510120

    【Abstract】 Objective To study the expressions of heat shock protein70(HSP70)in human heptocarcinoma cell line HepG2after hyperthermia and the inhibition effect of Quercetin on HSP70expresses.It will provide practical and theoretical foundation for clinical hyperthermia and traditional Chinese medicine on liver cancer.Methods (1)HepG2cells were cultured in vitro.HepG2cells were heat shocked being bathed in water at42℃stably for90min.(2)Immunocytochemistry and flow cytometry(FCM)were used.The expressions of HSP70were detected at0hour before and2 ......