【摘要】 目的 研究银杏叶提取物EGb761对肿瘤坏死因子-α(TNF-α)诱导Hela细胞凋亡和caspase-8活性的影响。方法 采用流式细胞术检测细胞凋亡，采用caspase-8荧光检测试剂盒测定caspase-8活性。结果 流式细胞术分析结果显示，EGb761在10～40mg/L终浓度范围内对rhTNF-α诱导的Hela细胞凋亡均有显著的抑制作用(P<0.01)，呈剂量依赖关系;caspase-8活性检测结果显示，EGb761在10～40mg/L终浓度范围内对rhTNF-α诱导的Hela细胞caspase-8活化均有显著的抑制作用(P<0.01)，呈剂量依赖关系。结论 结果提示，EGb761抑制TNF-α诱导的Hela细胞凋亡可能与其抑制caspase-8活化有关。

    关键词 肿瘤坏死因子-α EGb761 Hela细胞 凋亡 caspase-8

    Effects of Ginkgo biloba extract EGb761on apoptosis induced by TNF-αin Hela cell

    Huang Dinan，Hou Gan，Liu Wance

    Institute of Biochemistry and Molecular Biology，Guangdong Medical College，Zhanjiang Guangdong524023.

    【Abstract】 Objective To investigate the effects of Ginkgo biloba extract EGb761on apoptosis of Hela cell induced by tumor necrosis factor-α(TNF-α)and on the activity of caspase-8.Methods Flow cytometry was used to detect the effect of EGb761on the apoptosis of Hela cell induced by rhTNF-αand caspase-8fluorescent kits was used to detect the activity of caspase-8.Results The result of flow cytometry exhibited that EGb761with10～40mg/L final concentration had notable inhibition but not absolute inhibition on the apoptosis of Hela cell induced by rhTNF-α(P<0.01)and it showed dose-dependence.The detection of the activity of caspase-8showed that EGb761with10～40mg/L final concentration also had notable inhibition on the caspase-8activation of Hela cell inˉduced by rhTNF-α(P<0.01).Conclusion The inhibition of apoptosis in Hela cell induced by rhTNF-αby EGb761may have relation to the caspase-8activation. ......