【摘要】 目的 研究致动脉粥样硬化危险因素———氧化低密度脂蛋白(OX-LDL)与新的第二信使内源性一氧化碳(CO)及其合成酶血红素氧化酶-1(HO-1)的相互作用。 方法 通过反转录聚合酶链式反应、mRNA表达图像分析方法、放免法及生物化学等多项指标观察OX-LDL对培养血管内皮细胞中新的第二信使CO及其合成酶HO-1的作用。 结果 OX-LDL能刺激培养血管内皮细胞HO-1mRNA的表达及CO含量的增高，也能刺激内皮细胞ET-1mRNA表达增高。但加入氯化高铁血红素后，HO-1mRNA的表达增高，产生更多的CO及cGMP，从而导致ET-1mRNA表达减低。 结论 增强HO-1mRNA的表达及CO含量能降低由OX-LDL诱导的作用最强的血管收缩肽ET-1mRNA的表达。

    关键词 血红素氧化酶-1 内源性一氧化碳 氧化低密度脂蛋白

    The research of the interaction of OX-LDL with heme

    oxygenase-1in culture vascular endothelial cell

    Yu Lu

    Department of Kidney，305Hospital of People Liberation Army，Beijing100017.

    【Abstract】 Objective To research the interaction of the risk factor of guiding atherosclerosis-OX-LDL with the new second message-endogenous carbon monoxide and his synzyme heme oxygenase-.Methods Reverse transcription-polymerse chain reaction，dot blot and some biochemical index arks were performed so as to research the interaction of OX-LDL with endogenous carbon monoxide and his synzyme heme oxygenase-1.Results OX-LDL can incite the express of heme oxygenase-1mRNA and increase the concentration of endogenous carbon monox-ide and incite the express of ET-mRNA.After adding hemin ......