【摘要】 目的 研究黄芪丹参复方成分提高胎盘血供的分子机制，为临床有效防治胎盘血供不足所致妊娠并发症提供思路。 方法 提取分离有效中药黄芪、丹参复方成分，运用一氧化氮合酶(NOS)阻滞剂L-精氨酸甲酯(L-NAME)制作一氧化氮合成阻滞大鼠模型，透射电镜观察胎盘超微结构变化。 结果 模型组胎盘滋养细胞水肿，线粒体肿胀、空泡化明显，滋养细胞表面绒毛稀少，凋亡细胞增加，而中药治疗组明显较轻，凋亡数目较少，与空白组近似。 结论 黄芪丹参复方成分可能提高滋养细胞活力，阻抑滋养细胞异常凋亡，防止胎盘浅表着床，有利于滋养细胞的植入，从而达到提高胎盘血液供应的目的。

    关键词 凋亡 胎盘 滋养细胞 黄芪 丹参

    Study on the effects of the components of Mongolian milkvetch root and Dan-shen root compound on placental ultramicroscopic structure of nitrogen oxide synthesis blocking pregnant rats

    Li Chunmei，Wang Ruoguang，You Zhaoling，et al.

    Department of Intergrated Traditional and Western Medicine，Hunan Traditional Chinese Medicine College，Changsha410007.

    【Abstract】 Objective To provide thoughts on effectively preventing and treating pregnant complications caused by insufficient placental blood supply by exploring the molecular mechanism of the components of Mongolian milkvetch root and Dan-shen root compound elevating placental blood supply.Methods The effective components of Mongolian milkvetch root and Dan-shen root were separated and extracted.L-Arginine methyl ester(L-NAME)was applied to pregnant rats to make nitrogen oxide synthesis blocking model and then observed the change of placental ultramicroscopic structure by transmission electron microscope.Results Placental nourish cells of model group were dropsical ......