【摘要】 目的 在爪蟾卵母细胞中建立γ-干扰素活性位点(GAS)调控的绿色荧光蛋白GFP表达报告系统，探讨ox LDL对清道夫受体SR-A调控表达的影响，同时对丹参酮、川芎嗪等中药在其中的干预作用作出分析评价。 方法 将绿色荧光蛋白报告基因构建在调控清道夫受体SR-A基因表达的GAS调控元件的下游，并导入爪蟾卵母细胞。用ox LDL诱导的巨噬细胞条件培养液(MCM)和平滑肌细胞条件培养液(SCM)以及含有γ-干扰素、丹参酮和川芎嗪的条件培养液培养注射后的卵母细胞;3天后，观察绿色荧光蛋白的表达情况。 结果 MCM和SCM都增加了系统中GFP的表达;γ-干扰素、丹参酮和川芎嗪均有抑制SCM诱导的作用。 结论 丹参酮和川芎嗪与γ-干扰素有类似的作用，可以通过干预GAS调控途经，阻止ox LDL诱导引起的SR-A过量表达。

    【关键词】 爪蟾卵母细胞 清道夫受体 γ-干扰素活性位点 丹参酮 川芎嗪

    Establishment and application ofγ-interferon activation site regulatory report system in xenopus oocytes

    Fan Chunlei，Gao Liping，Wo Xingde，et al.

    Life Science Department，Zhejiang College of Traditional Chinese Medicine，Zhejiang310053.

    【Abstract】 Objective To investigate the effect of oxLDL on the expression of SR-A，and evaluate the in-tervention action of anti-atherosclerosis drugs on it，we first established the novelγ-interferon activation site(GAS)elements regulatory GFP report systems in the xenopus oocytes.Methods A plasmid，in which green fluo-rescence protein gene was constructed downstream from the GAS elements，was microinjected into the nucleus of xeno-pus oocytes.The oocytes then were cultured in the smooth muscle cell conditioned medium(SCM) ......