【摘要】 目的 研究前列腺增生症(BPH)和前列腺增生症伴急性尿潴留患者一氧化氮(NO)含量和前列腺部尿道组织中一氧化氮合成酶(NOS)活性。 方法 制备NOS抽提液，用双波长分光光度计在401nm和421nm波长下测定吸光度差异的变化代表NOS含量。取血用一氧化氮试剂盒(酶法)测定血NO含量。 结果 在BPH急性尿潴留时和单纯BPH时分别为(122.25±1.04)μmol/L和(71.5±1.04)μmol/L，两者相比，差异有非常显著意义(P<0.01)。BPH伴急性尿潴留患者组织中NOS活性为(106.56±24.52)pmol·mg -1 ·min -1 ，单纯BPH患者组织中NOS活性为(250.23±112.59)pmol·mg -1 ·min -1 (P<0.001)。 结论 NO参与调节前列腺部尿道组织的松弛，NOS活性的降低，导致NO降低，可能由此导致临床上BPH出现急性尿潴留。

    关键词 前列腺增生症 一氧化氮合成酶 急性尿潴留 一氧化氮

    Study on the content of NO and the activity of NOS in the patients with BPH accompanying the acute urine retention

    Wang Zhong，Lai Yulian

    The Urology Department of No.2People's Hospital of Fujian Province，Fujian350000.

    【Abstract】 Objective To investigate the content of NO and the activity of NOS in BPH and BPH accompany-ing the acute urine retention.Methods To produce liquor containing NOS and to estimate the difference of401nm and421nm by spectrophotometer deputy the content of NOS.To assay the content of NO by nitrogen monoxide test dose box.Results The contents of NO in patients with BPH accompanying the acute urine retention is(122.25±1.04)μmol/L ......