【摘要】 目的 观察银杏叶提取物EGb761体外抗β-淀粉样蛋白(Aβ)损伤的作用。 方法 用MTT法检测细胞的增殖活性;释放到细胞外的LDH活性检测细胞膜的损伤;Caspase-3活性检测细胞凋亡。 结果 EGb761呈剂量依赖性提高Aβ损伤引起的SY5Y细胞的存活率降低，降低Aβ损伤引起的LDH释放及Caspase-3活性增加。 结论 EGb761对Aβ 25-35 损伤有保护作用。

    关键词 β-淀粉样蛋白 银杏叶提取物(EGb761)

    Effects of EGb761on SH-SY5Y cells against damage induced by Aβ

    Wang Yuehua，Du Guanhua

    Institute of Materia Medica，Chinese Academy of Medical Science and Peking Union Medical College，Beijing100050.

    【Abstract】 Objective To investigate the protective effects of EGb761on SH-SY5Y cells against damage induced by amyloidβ-peptide(Aβ).Methods The viability of SH-SY5Y cells was measured byMTT assay.The loss of membrane integrity and cell lysis was measured by assay the release of lactic dehydrogenase(LDH).The activ-ity of caspase-3in cells was measured by means of the cleavage of fluorogenic substrate Ac-DEVD-MCA.Results EGb761could respectively improve the survival of SH-SY5Y cells.The results also showed that EGb761signifi-cantly decreased Aβ-induced LDH release and reduced the Aβ 25-35 -induced increase of caspase-3activity.Con-clusion EGb761protected SH-SY5Y cells against toxicity induced by Aβ. ......