吴清明,陈滋华,童强,李胜保,王小虎,郧阳医学院附属太和医院消化内科 湖北省石堰市 442000

    吴清明,男, 1965-5-25生, 湖北黄冈人,汉族,2004年毕业于武汉大学,博士, 教授.

    通讯作者:吴清明,442000, 湖北省石堰市,郧阳医学院附属太和医院消化内科. pollex001@163.com

    电话:0719-6869066

    收稿日期:2004-12-27 接受日期: 2005-01-14

    Effectof Seleno Methionine on P-glycoprotein in cisplatin-resistant esophagealcarcinoma cells

    Qing-Ming Wu, Zi-Hua Chen, Qiang Tong, Shen-Bao Li, Xiao-Hu Wang

    Qing-Ming Wu, Zi-Hua Chen, Qiang Tong, Shen-Bao Li, Xiao-Hu Wang,Department of gasteoenterology, the Affiliated Taihe Hospital of YunyangMedical College, Shiyan 442000, Hubei Province, China

    Correspondence to: Dr. Qing-Ming Wu, Department ofgasteoenterology, the Affiliated Taihe Hospital of Yunyang MedicalCollege, Shiyan 442000, Hubei Province, China. pollex001@163.com

    Received: 2004-12-27 Accepted: 2005-01-14

    Abstract

    AIM: To investigate the inhibitory effect of Seleno Methionine (SM) onthe P-glycoprotein in cisplatin(DDP)-resistant esophageal carcinoma cells.

    METHODS: High concentration of DDP was used intermittantly toinduce drug-resistant esophageal carcinoma cells EC9706. The effect of SMon the sensibility of cells to DDP was detected by MTT.Immunohistochemical technique was used to detect the expression of P-glycoproteinand then semi-quantative analysis was performed. ......