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小青龙汤对于过敏气喘动物之免疫调控的影响
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     过去传统中药对于气喘的处方及临床实验都有记载,小青龙汤(Xiao-Qing-Long-Tang, XQLT)是中药其中一种用来治疗气喘和过敏性鼻炎,但是此药方之治疗机制至目前仍不十分明确。

    本研究是利用过去所建立的过敏气喘动物模式,分析此过敏气喘之小鼠的肺泡冲洗液,来探讨小青龙汤的免疫调控作用。

    本实验以Der p来致敏小鼠后,再以每公斤体重1克的小青龙汤萃取物喂食小鼠,实验分组包括A组:致敏前1小时喂食BALB/c小鼠每公斤体重1克的小青浓汤;B组:致敏后24小时喂食BALB/c小鼠每公斤体重1克的小青浓汤;C组:致敏前每隔一日喂食BALB/c小鼠每公斤体重1克的小青浓汤,共计喂食六次。经由以上实验来评估短期或长期喂食小青龙汤对气管过敏反应之预防效果,本实验将针对致敏小鼠之肺泡冲洗液的细胞数目和以流式细胞仪分析淋巴球族群之变化。

    致敏前1小时或每隔一日喂食BALB/c小鼠每公斤体重1克的小青浓汤,共计喂食六次,肺泡冲洗液的嗜伊红性细胞数目及百分比会减少,达到抑制气管发炎的效果,并降低肺部间质淋巴球之数目,小青龙汤的免疫调控作用是经由活化及提升CD4和双阴性T淋巴球之族群的细胞数目来达成,但小青龙汤对于B组之小鼠也就是已有过敏反应发生情况下则不具此效用。
, 百拇医药
    小青龙汤证实在致敏前有免疫调控的作用,在致敏后有降低支气管发炎的功能。

    关键词:小青龙 中国草药俗称中药 支气管气喘

    Code Number:CCMP87-RD-033

    Title:The Immunomodulate Effect of Xiao-Qing-Long-Tong on Mite-sensitioe Allergic Mice

    Orgazination:Division of Clinical Immunology, Department of Pediatrics, College of Medicine National Cheng-Kung University, Tainan, TAIWAN

    Auther:Jiu-Yao Wang, M.D., D.Phi.
, 百拇医药
    Allergic asthma is the most common chronic disease in children. The prevalence of this disease is increased rapidly in recent ten years and the cause of increased incidence is unknown either. There were detailed descriptions about the pathologic mechanisms, symptoms, clinical experiences and therapeutic prescriptions of asthma in the traditional Chinese medicine. However, many therapeutic mechanisms are still unclear. Previously, there were studies that used animal model to investigate the therapeutic mechanisms of Xiao-Qing-Long-Tang which also frequently used in clinics. The results showed that Xiao-Qing-Long-Tang (XQLT) was useful to relieve the early and late asthmatic reactions and reduce the local infiltration of inflammatory cells. This study was proceeded according to the methods of our previous reports. We found that chronic use of XQLT before allergen challenged can inhibit the allergen-induced bronchial inflammation, while after allergen challenge, XQLT will exert harmful effect by aggravate eosinophils infiltration and bronchial inflammation. There were significant decrease percentages of eosinophils and macrophages, and increase of lymphocytes population in BAL of group A (feeding XQLT 30 min before allergen challenge) as compared to those of PBS group. While the cellular distribution in BAL of group B (feeding XQLT 24 hr after AC) and group C (long term use of XQLT) had no significant change as compared to that of PBS group. BAL cells from allergen-challenged mice were also assessed with intracellular staining by FAScan. Mononuclear cells obtained from peripheral blood and peribronchial lymph node were analyzed for the response to XQLT in vitro. There was significant increase in the percentage of CD3+/CD4+/CD8- lymphocytes in the group A mice as compared to those of PBS group mice. Moreover, the percentage of double negative (CD4-/CD8-) T lymphocytes in the BAL of sensitized mice were also increased when treated with XQLT 30 min before AC. We suggested that the therapeutic mechanisms of Xiao-Qing-Long-Tang were through change of Th2/Th1 cytokine profile that induced immunological tolerance in the airway and reduced some degree of allergic inflammation. Therefore, in the future study, we plan to study firstly the immunomodulatory effect of XQLT on the allergen-induced bronchial inflammation after long term used of this herb medication., 百拇医药