沈仓良, 陈伟华, 邹思湘, 南京农业大学动物医学院 江苏省南京市 210095

    沈仓良, 男, 1981-05-18生, 江苏省苏州市人, 汉族. 2004年南京农业大学动物医学院硕士生, 从事动物营养生化研究.

    通讯作者: 邹思湘, 210095, 江苏省南京市, 南京农业大学动物医学院. sixiangzou@njau.edu.cn

    电话: 025-84396763 传真:025-4398669

    收稿日期: 2005-03-22 接受日期: 2005-04-01

    Anti-infection effect of hydrolysates from conglycinin on E.coli in mice

    Cang-Liang Shen, Wei-Hua Chen, Si-Xiang Zou

    Cang-Liang Shen, Wei-Hua Chen, Si-Xiang Zou, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China

    Correspondence to: Si-Xiang Zou, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China. sixiangzou@njau.edu.cn

    Received: 2005-03-22 Accepted: 2005-04-01

    Abstract

    AIM: To elucidate anti-infection effect of hydrolysates from conglycinin on E.coli in mice.

    METHODS: Male KM mice were assigned randomly in five treatments. After feeding basal diet three days, E.coliO₁₃₈ was fed in five treatments from 2×10¹¹ to 2×10⁷CFU/L, diluted (in decuple) ordinally. The LD₅₀ of mice feeding E.coliO₁₃₈ after 56 h was 4.73×10¹⁰ CFU/L. Two batch of Male KM mice were randomly assigned to six treatments respectively: normal (NOR) group, feeding-E.coli control (FEC), Basal diet + physiological saline, Basal diet + Hcl-full hydrolysis of conglycinin (HCL-FHC), Basal diet + conglycinin (Conglycinin), Basal diet + pepsin-hydrolysate conglycinin (PTC), and Basal diet + purified fraction B of pepsin hydrolysates of conglycinin (P2-PTC) group. The mice were fed with a dose of 0.2 mL /g (containing equal amount of nitrogen) except the ones in NOR and FEC group. Twenty days after feeding, each mouse in FEC, HCL-FHC, Conglycinin, PTC and P2-PTC group was fed with E.coliO₁₃₈ (2×10⁹ CFU/L and 2×10¹¹ CFU/L for the two batch, respectively, 0.2 mL/g) in the 22^nd day (midday). The actions of the mice were observed until 48 h after feeding with E.coliO₁₃₈. Then all the mice were killed. The blood, spleen and the whole length of intestines were collected. The immune index was determined by radioimmunoassay. ......