急性胰腺炎细胞凋亡的研究进展
张喜平, 杭州市第一人民医院普通外科 浙江省杭州市 310006
刘达人, 田华, 浙江大学附属第二医院普通外科 浙江省杭州市 310008
浙江省医药卫生科技计划项目资助课题, No. 2003B134
浙江省中医药卫生科技计划项目资助课题, No.2003C130
杭州市重大科技发展计划项目资助课题, No. 2003123B19
杭州市医药卫生重点科技计划项目资助课题, No. 2003A004
通讯作者: 张喜平, 310006, 浙江省杭州市浣纱路261号, 杭州市第一人民医院普外科. zxp99688@sina.com
, 百拇医药
电话: 0571-87065701
收稿日期: 2005-04-04 接受日期: 2005-04-09
摘要
细胞凋亡又称程序性细胞死亡，是细胞由一系列基因调节控制的自主性的有序死亡.细胞凋亡的形态学上主要表现为细胞皱缩、染色质浓染和出现凋亡小体.凋亡小体具有完整的膜结构，内含部分细胞质、细胞器和破碎的细胞核成分，形成的凋亡小体由邻近的正常细胞和吞噬细胞清除，其内容物不会因外泄而引起炎症反应.急性胰腺炎与细胞凋亡关系方面的研究起步较晚，许多机制未能完全阐明，本文针对急性胰腺炎细胞凋亡和调控的研究进展进行综述.
张喜平, 刘达人, 田华. 急性胰腺炎细胞凋亡的研究进展. 世界华人消化杂志 2005;13(11):1340-1343
, 百拇医药
参考文献
1 Kaiser AM, Saluja AK, Sengupta A, Saluja M, Steer ML. Relationshipbetween severity, necrosis, and apoptosis in
five models of experimental acutepancreatitis. Am J Physiol 1995;269(5Pt 1):1295-1304
2 Samuilov VD, Oleskin AV, Lagunova EM. Programmed cell death. Biochemistry(Mosc) 2000;65:873-887
3 Bhatia M, Wallig MA, Hofbauer B, Lee HS, Frossard JL, Steer ML,Saluja AK. Induction of apoptosis in pancreatic
, 百拇医药
acinar cells reduces the severity of acutepancreatitis. Biochem Biophys Res Commun 1998;246:476-483
4 Saluja A, Hofbauer B, Yamaguchi Y, Yamanaka K, Steer M. Inductionof apoptosis reduces the severity of
caerulein-induced pancreatitis in mice. BiochemBiophys Res Commun 1996;220:875-878
5 He ZJ, Podkletnova I, Alho H, Sand J, Nordback I. Apoptosis inacute pancreatitis. Ann Chir Gynaecol 2000;89:65-67
, http://www.100md.com
6 袁耀宗, 龚自华, 楼恺娴, 涂水平, 翟祖康, 徐家裕. 生长抑素及Octreotide对急性胰腺炎胰腺细胞凋亡的作用机制.
中国危重病急救医学 2000;12:402-405
7 钟荣德, 代伟, 周杰. 生长抑素对重症胰腺炎胰腺细胞凋亡的影响. 广东医学 2004;25:138-140
8 Krajewski S, Krajewska M, Shabaik A, Miyashita T, Wang HG, Reed JC.Immunohistochemical determination of in
vivo distribution of Bax, a dominantinhibitor of Bcl-2. Am J Pathol 1995;145:1323-1336
, 百拇医药
9 Gomez G, Lee HM, He Q, Englander EW, Uchida T, Greeley GH Jr. Acutepancreatitis signals activation
of apoptosis-associated and survival genesin mice. Exp Biol Med (Maywood) 2001;226:692-700
10 Nakazawa T, Agematsu K, Yabuhara A. Later development of Fas ligand-mediatedcytotoxicity as compared
with granule-mediated cytotoxicityduring the maturation of natural killer cells. Immunology 1997;92:180-187
, http://www.100md.com
11 Uehara S, Gothoh K, Handa H, Tomita H, Tomita Y. Immune function inpatients with acute pancreatitis. J
Gastroenterol Hepatol 2003;18:363-370
12 Yasuda H, Kataoka K, Ichimura H, Mitsuyoshi M, Iida T, Kita M,Imanishi J. Cytokine expression and induction of acinar
cell apoptosis after pancreatic ductligation in mice. J Interferon Cytokine Res 1999;19:637-644
, http://www.100md.com
13 Wada M, Doi R, Hosotani R, Lee JU, Imamura M. Apoptosis of acinarcells in rat pancreatic duct ligation.
J Gastroenterol 1995;30:813-814
14 Schuler M, Green DR. Mechanisms of p53-dependent apoptosis. BiochemSoc Trans 2001;29(Pt 6):684-688
15 Masamune A, Sakai Y, Satoh A, Fujita M, Yoshida M, Shimosegawa T.Lysophosphatidylcholine induces apoptosis
in AR42J cells. Pancreas 2001;22:75-83
, http://www.100md.com
16 Norman J, Yang J, Fink G, Carter G, Ku G, Denham W, Livingston D.Severity and mortality of experimental
pancreatitis are dependent oninterleukin-1 converting enzyme (ICE). J Interferon Cytokine Res 1997;17:113-118
17 Yang J, Fier A, Carter Y, Liu G, Epling-Burnette PK, Bai F,Loughran TP Jr, Mastorides S, Norman JG, Murr MM. Liver
injury during acute pancreatitis: therole of pancreatitis-associated ascitic fluid (PAAF), p38-MAPK, andcaspase-3
, 百拇医药
in inducing hepatocyte apoptosis.J Gastrointest Surg 2003;7:200-207
18 Ortiz EM, Dusetti NJ, Vasseur S, Malka D, Bodeker H, Dagorn JC,Iovanna JL. The pancreatitis-associated protein
is induced by free radicals in AR4-2Jcells and confers cell resistance to apoptosis. Gastroenterology 1998;114:808-816
19 Hughes CB, Gaber LW, Mohey el-Din AB, Grewal HP, Kotb M, Mann L,Gaber AO. Inhibition of TNF alpha improves
, http://www.100md.com
survival in an experimental model ofacute pancreatitis. Am Surg 1996;62:8-13
20 Fink G, Yang J, Carter G, Norman J. Acute pancreatitis-inducedenzyme release and necrosis are attenuated by
IL-1 antagonism through an indirectmechanism. J Surg Res 1997;67:94-97
21 Norman JG, Fink G, Franz M, Guffey J, Carter G, Davison B, SextonC, Glaccum M. Active interleukin-1 receptor
, http://www.100md.com
required for maximal progression ofacute pancreatitis. Ann Surg 1996;223:163-169
22 Kusske AM, Rongione AJ, Reber HA. Cytokines and acute pancreatitis.Gastroenterology 1996;110:639-642
23 Inagaki T, Hoshino M, Hayakawa T, Ohara H, Yamada T, Yamada H, IidaM, Nakazawa T, Ogasawara T, Uchida
A, Hasegawa C, Miyaji M, Takeuchi T.Interleukin-6 is a useful marker for early prediction of the severity of
, 百拇医药
acute pancreatitis. Pancreas 1997;14:1-8
24 郝建宇, 王世鑫, 关玉盘, 杨昭徐. 大鼠急性胰腺炎模型中血清白介素-6及白介素-8的变化. 中华消化杂志 2000;20:68-69
25 Molero X, Guarner F, Salas A, Mourelle M, Puig V, Malagelada JR.Nitric oxide modulates pancreatic basal secretion
and response to cerulein in the rat:effects in acute pancreatitis. Gastroenterology 1995;108:1855-1862
26 Werner J, Fernandez-del Castillo C, Rivera JA, et al. On theprotective mechanisms of nitric oxide in acute
, 百拇医药
pancreatitis. Gut 1998;43:401-407
27 Konturek SJ, Szlachcic A, Dembinski A, Warzecha Z, Jaworek J,Stachura J. Nitric oxide in pancreatic secretion
and hormone induces pancreatitis inrats. Int J Pancreatol 1994;15:19-28
28 Dobosz M, Hac S, Wajda Z. Does nitric oxide protect frommicrocirculatory disturbances in experimental acute
pancreatitis in rats? Int JMicrocirc Clin Exp 1996;16:221-226
, http://www.100md.com
29 Wada M, Doi R, Hosotani R, Lee JU, Fujimoto K, Koshiba T, MiyamotoY, Fukuoka S, Imamura M. Expression of Bcl-2
and PCNA in duct cells afterpancreatic duct ligation in rats. Pancreas 1997;15:176-182
30 Fujimoto K, Hosotani R, Wada M, Lee J, Koshiba T, Miyamoto Y, DoiR, Imamura M. Ischemia-reperfusion injury on
the pancreas in rats: identificationof acinar cell apoptosis. J Surg Res 1997;71:127-136
, http://www.100md.com
31 Kimura K, Shimosegawa T, Sasano H, Abe R, Satoh A, Masamune A,Koizumi M, Nagura H, Toyota T.
Endogenous glucocorticoids decreasethe acinar cell sensitivity to apoptosis during cerulein pancreatitis in
rats. Gastroenterology 1998;114:372-381
32 袁耀宗.胰腺病学新进展与新技术. 上海: 上海科技文献出版社, 2000:106
33 陈元方.胃肠肽类激素基础与临床. 北京: 北京医科大学和中国协和医科大学联合出版社, 1997:399-422
, 百拇医药
34 刘建生, 卫新革, 付极, 刘进, 袁耀宗, 吴云林. 急性胰腺炎与内皮素、一氧化氮和氧自由基关系研究.
中国医师杂志 2003;5:28-29
35 李淑华, 李晓霞, 初彦辉, 冯芹喜, 胡大鹏, 关利新, 李志强, 胡怀明.硝普钠对急性胰腺炎大鼠治疗作用初探.
中国病理生理杂志 2000;16:852-853
36 Rakonczay Z Jr, Jarmay K, Kaszaki J, Mandi Y, Duda E, Hegyi P,Boros I, Lonovics J, Takacs T. NF-kappaB activation
is detrimental in arginine-inducedacute pancreatitis. Free Radic Biol Med 2003;34:696-709
, http://www.100md.com
37 Telek G, Feher J, Jakab F, Claude R. Acute pancreatitis: recentadvances in understanding its pathophysiology. Orv
Hetil 2000;141:267-278
38 Hill DB, Barve S, Joshi-Barve S, McClain C. Increased monocytenuclear factor-kappaB activation and tumor
necrosis factor production inalcoholic hepatitis. J Lab Clin Med 2000;135:387-395
39 左伟. 核因子-kB活化在大鼠急性坏死性胰腺炎肺损伤中的作用. 实用医学杂志 2003;19:465-466
, 百拇医药
40 裴红红, 秦兆寅, 杨正安, 封英群. 大鼠急性坏死性胰腺炎时核因子kB 表达的意义. 中华普通外科杂志 2002;17:752
41 裴红红, 秦兆寅, 杨正安, 封英群. 核因子-kB 在两种实验性胰腺炎时表达的意义. 中国急救医学 2002;22:72-73
42 Hahm KB, Kim JH, You BM. Induction of apoptosis with an extract ofArtemisia asiatica attenuates the severity
of cerulein-induced pancreatitis inrats. Pancreas 1998;17:153-157
43 Kimura K, Shimosegawa T, Abe R. Low doses of lipopolysaccharideupregulate acinar cell apoptosis in
cerulein pancreatitis. Pancreas 1998;17:120-126
编辑 徐协群 审读 张海宁, http://www.100md.com( 张喜平,刘达人,田 华)
刘达人, 田华, 浙江大学附属第二医院普通外科 浙江省杭州市 310008
浙江省医药卫生科技计划项目资助课题, No. 2003B134
浙江省中医药卫生科技计划项目资助课题, No.2003C130
杭州市重大科技发展计划项目资助课题, No. 2003123B19
杭州市医药卫生重点科技计划项目资助课题, No. 2003A004
通讯作者: 张喜平, 310006, 浙江省杭州市浣纱路261号, 杭州市第一人民医院普外科. zxp99688@sina.com
, 百拇医药
电话: 0571-87065701
收稿日期: 2005-04-04 接受日期: 2005-04-09
摘要
细胞凋亡又称程序性细胞死亡，是细胞由一系列基因调节控制的自主性的有序死亡.细胞凋亡的形态学上主要表现为细胞皱缩、染色质浓染和出现凋亡小体.凋亡小体具有完整的膜结构，内含部分细胞质、细胞器和破碎的细胞核成分，形成的凋亡小体由邻近的正常细胞和吞噬细胞清除，其内容物不会因外泄而引起炎症反应.急性胰腺炎与细胞凋亡关系方面的研究起步较晚，许多机制未能完全阐明，本文针对急性胰腺炎细胞凋亡和调控的研究进展进行综述.
张喜平, 刘达人, 田华. 急性胰腺炎细胞凋亡的研究进展. 世界华人消化杂志 2005;13(11):1340-1343
, 百拇医药
参考文献
1 Kaiser AM, Saluja AK, Sengupta A, Saluja M, Steer ML. Relationshipbetween severity, necrosis, and apoptosis in
five models of experimental acutepancreatitis. Am J Physiol 1995;269(5Pt 1):1295-1304
2 Samuilov VD, Oleskin AV, Lagunova EM. Programmed cell death. Biochemistry(Mosc) 2000;65:873-887
3 Bhatia M, Wallig MA, Hofbauer B, Lee HS, Frossard JL, Steer ML,Saluja AK. Induction of apoptosis in pancreatic
, 百拇医药
acinar cells reduces the severity of acutepancreatitis. Biochem Biophys Res Commun 1998;246:476-483
4 Saluja A, Hofbauer B, Yamaguchi Y, Yamanaka K, Steer M. Inductionof apoptosis reduces the severity of
caerulein-induced pancreatitis in mice. BiochemBiophys Res Commun 1996;220:875-878
5 He ZJ, Podkletnova I, Alho H, Sand J, Nordback I. Apoptosis inacute pancreatitis. Ann Chir Gynaecol 2000;89:65-67
, http://www.100md.com
6 袁耀宗, 龚自华, 楼恺娴, 涂水平, 翟祖康, 徐家裕. 生长抑素及Octreotide对急性胰腺炎胰腺细胞凋亡的作用机制.
中国危重病急救医学 2000;12:402-405
7 钟荣德, 代伟, 周杰. 生长抑素对重症胰腺炎胰腺细胞凋亡的影响. 广东医学 2004;25:138-140
8 Krajewski S, Krajewska M, Shabaik A, Miyashita T, Wang HG, Reed JC.Immunohistochemical determination of in
vivo distribution of Bax, a dominantinhibitor of Bcl-2. Am J Pathol 1995;145:1323-1336
, 百拇医药
9 Gomez G, Lee HM, He Q, Englander EW, Uchida T, Greeley GH Jr. Acutepancreatitis signals activation
of apoptosis-associated and survival genesin mice. Exp Biol Med (Maywood) 2001;226:692-700
10 Nakazawa T, Agematsu K, Yabuhara A. Later development of Fas ligand-mediatedcytotoxicity as compared
with granule-mediated cytotoxicityduring the maturation of natural killer cells. Immunology 1997;92:180-187
, http://www.100md.com
11 Uehara S, Gothoh K, Handa H, Tomita H, Tomita Y. Immune function inpatients with acute pancreatitis. J
Gastroenterol Hepatol 2003;18:363-370
12 Yasuda H, Kataoka K, Ichimura H, Mitsuyoshi M, Iida T, Kita M,Imanishi J. Cytokine expression and induction of acinar
cell apoptosis after pancreatic ductligation in mice. J Interferon Cytokine Res 1999;19:637-644
, http://www.100md.com
13 Wada M, Doi R, Hosotani R, Lee JU, Imamura M. Apoptosis of acinarcells in rat pancreatic duct ligation.
J Gastroenterol 1995;30:813-814
14 Schuler M, Green DR. Mechanisms of p53-dependent apoptosis. BiochemSoc Trans 2001;29(Pt 6):684-688
15 Masamune A, Sakai Y, Satoh A, Fujita M, Yoshida M, Shimosegawa T.Lysophosphatidylcholine induces apoptosis
in AR42J cells. Pancreas 2001;22:75-83
, http://www.100md.com
16 Norman J, Yang J, Fink G, Carter G, Ku G, Denham W, Livingston D.Severity and mortality of experimental
pancreatitis are dependent oninterleukin-1 converting enzyme (ICE). J Interferon Cytokine Res 1997;17:113-118
17 Yang J, Fier A, Carter Y, Liu G, Epling-Burnette PK, Bai F,Loughran TP Jr, Mastorides S, Norman JG, Murr MM. Liver
injury during acute pancreatitis: therole of pancreatitis-associated ascitic fluid (PAAF), p38-MAPK, andcaspase-3
, 百拇医药
in inducing hepatocyte apoptosis.J Gastrointest Surg 2003;7:200-207
18 Ortiz EM, Dusetti NJ, Vasseur S, Malka D, Bodeker H, Dagorn JC,Iovanna JL. The pancreatitis-associated protein
is induced by free radicals in AR4-2Jcells and confers cell resistance to apoptosis. Gastroenterology 1998;114:808-816
19 Hughes CB, Gaber LW, Mohey el-Din AB, Grewal HP, Kotb M, Mann L,Gaber AO. Inhibition of TNF alpha improves
, http://www.100md.com
survival in an experimental model ofacute pancreatitis. Am Surg 1996;62:8-13
20 Fink G, Yang J, Carter G, Norman J. Acute pancreatitis-inducedenzyme release and necrosis are attenuated by
IL-1 antagonism through an indirectmechanism. J Surg Res 1997;67:94-97
21 Norman JG, Fink G, Franz M, Guffey J, Carter G, Davison B, SextonC, Glaccum M. Active interleukin-1 receptor
, http://www.100md.com
required for maximal progression ofacute pancreatitis. Ann Surg 1996;223:163-169
22 Kusske AM, Rongione AJ, Reber HA. Cytokines and acute pancreatitis.Gastroenterology 1996;110:639-642
23 Inagaki T, Hoshino M, Hayakawa T, Ohara H, Yamada T, Yamada H, IidaM, Nakazawa T, Ogasawara T, Uchida
A, Hasegawa C, Miyaji M, Takeuchi T.Interleukin-6 is a useful marker for early prediction of the severity of
, 百拇医药
acute pancreatitis. Pancreas 1997;14:1-8
24 郝建宇, 王世鑫, 关玉盘, 杨昭徐. 大鼠急性胰腺炎模型中血清白介素-6及白介素-8的变化. 中华消化杂志 2000;20:68-69
25 Molero X, Guarner F, Salas A, Mourelle M, Puig V, Malagelada JR.Nitric oxide modulates pancreatic basal secretion
and response to cerulein in the rat:effects in acute pancreatitis. Gastroenterology 1995;108:1855-1862
26 Werner J, Fernandez-del Castillo C, Rivera JA, et al. On theprotective mechanisms of nitric oxide in acute
, 百拇医药
pancreatitis. Gut 1998;43:401-407
27 Konturek SJ, Szlachcic A, Dembinski A, Warzecha Z, Jaworek J,Stachura J. Nitric oxide in pancreatic secretion
and hormone induces pancreatitis inrats. Int J Pancreatol 1994;15:19-28
28 Dobosz M, Hac S, Wajda Z. Does nitric oxide protect frommicrocirculatory disturbances in experimental acute
pancreatitis in rats? Int JMicrocirc Clin Exp 1996;16:221-226
, http://www.100md.com
29 Wada M, Doi R, Hosotani R, Lee JU, Fujimoto K, Koshiba T, MiyamotoY, Fukuoka S, Imamura M. Expression of Bcl-2
and PCNA in duct cells afterpancreatic duct ligation in rats. Pancreas 1997;15:176-182
30 Fujimoto K, Hosotani R, Wada M, Lee J, Koshiba T, Miyamoto Y, DoiR, Imamura M. Ischemia-reperfusion injury on
the pancreas in rats: identificationof acinar cell apoptosis. J Surg Res 1997;71:127-136
, http://www.100md.com
31 Kimura K, Shimosegawa T, Sasano H, Abe R, Satoh A, Masamune A,Koizumi M, Nagura H, Toyota T.
Endogenous glucocorticoids decreasethe acinar cell sensitivity to apoptosis during cerulein pancreatitis in
rats. Gastroenterology 1998;114:372-381
32 袁耀宗.胰腺病学新进展与新技术. 上海: 上海科技文献出版社, 2000:106
33 陈元方.胃肠肽类激素基础与临床. 北京: 北京医科大学和中国协和医科大学联合出版社, 1997:399-422
, 百拇医药
34 刘建生, 卫新革, 付极, 刘进, 袁耀宗, 吴云林. 急性胰腺炎与内皮素、一氧化氮和氧自由基关系研究.
中国医师杂志 2003;5:28-29
35 李淑华, 李晓霞, 初彦辉, 冯芹喜, 胡大鹏, 关利新, 李志强, 胡怀明.硝普钠对急性胰腺炎大鼠治疗作用初探.
中国病理生理杂志 2000;16:852-853
36 Rakonczay Z Jr, Jarmay K, Kaszaki J, Mandi Y, Duda E, Hegyi P,Boros I, Lonovics J, Takacs T. NF-kappaB activation
is detrimental in arginine-inducedacute pancreatitis. Free Radic Biol Med 2003;34:696-709
, http://www.100md.com
37 Telek G, Feher J, Jakab F, Claude R. Acute pancreatitis: recentadvances in understanding its pathophysiology. Orv
Hetil 2000;141:267-278
38 Hill DB, Barve S, Joshi-Barve S, McClain C. Increased monocytenuclear factor-kappaB activation and tumor
necrosis factor production inalcoholic hepatitis. J Lab Clin Med 2000;135:387-395
39 左伟. 核因子-kB活化在大鼠急性坏死性胰腺炎肺损伤中的作用. 实用医学杂志 2003;19:465-466
, 百拇医药
40 裴红红, 秦兆寅, 杨正安, 封英群. 大鼠急性坏死性胰腺炎时核因子kB 表达的意义. 中华普通外科杂志 2002;17:752
41 裴红红, 秦兆寅, 杨正安, 封英群. 核因子-kB 在两种实验性胰腺炎时表达的意义. 中国急救医学 2002;22:72-73
42 Hahm KB, Kim JH, You BM. Induction of apoptosis with an extract ofArtemisia asiatica attenuates the severity
of cerulein-induced pancreatitis inrats. Pancreas 1998;17:153-157
43 Kimura K, Shimosegawa T, Abe R. Low doses of lipopolysaccharideupregulate acinar cell apoptosis in
cerulein pancreatitis. Pancreas 1998;17:120-126
编辑 徐协群 审读 张海宁, http://www.100md.com( 张喜平,刘达人,田 华)
