【摘要】 目的 探讨S-期激酶相关蛋白(Skp2)表达与胃癌临床病理学特征间的关系。 方法 利用半定量RT-PCR和免疫组化Envision方法检测Skp2mRNA和蛋白在胃癌及正常组织中的表达。 结果 50例胃癌组织中Skp2mRNA的表达量明显高于正常组织(ω=688，P<0.01)。pT 3～4 胃癌组织中COX-2mRNA的表达明显高于pT 1～2 胃癌组织(ω=352，P<0.01)。60份胃癌石蜡标本中有42份Skp2阳性表达，阳性率为70.0%。Skp2阳性表达与浸润深度、淋巴结转移、UICC分期显著相关(P<0.01)，与组织学类型、脉管侵犯无关。 结论 Skp2在胃癌的发生发展过程中起着重要作用，其高表达提示患者预后不良。

    关键词 胃癌 S-期激酶相关蛋白 基因

    Expression and clinical significance of S-phase kinase-associated protein2in gastric cancer

    Wang Xingping，Yu Jiren，Chen Guosheng，et al.

    Department of Surgery，Yuqian Peoples Hospital of Linan，Zhejiang311311.

    【Abstract】 Objective To study the relationship between expression of S-phase kinase-associated protein2(Spk2)and the clinicopathological feature in gastric cancer.Methods The Spk2mRNA and protein were examined in gastric cancer tissue and normal tissue by semi-quantitative RT-PCR and immunohistochemistry.Results The expression of Spk2mRNA in gastric cancer tissue was significantly higher than that of nomal tissue in50cases(ω=688 ......