CD14基因C-260T多态性与溃疡性结肠炎的相关性
郭秋莎,夏冰,武汉大学中南医院综合医疗科,武汉大学中南医院消化病研究中心 湖北省武汉市 430071
国家自然科学基金资助课题,No. 30370638
湖北省卫生厅重点项目(2003)
通讯作者:夏冰,430071, 湖北省武汉市东湖路169号,武汉大学中南医院综合医疗科，武汉大学中南医院消化病研究中心. bingxia2004@yahoo.com.cn
电话: 027-67812985 传真:027-87330795
收稿日期:2005-04-04 接受日期:2005-04-09
, http://www.100md.com
摘要目的:研究我国湖北汉族人CD14基因C-260T多态性的分布及CD14基因C-260T位点的突变是否与溃疡性结肠炎(UC)相关.
方法:采用PCR-RFLP方法检测114例UC患者以及160例正常对照者CD14-260基因型及等位基因频率.
结果:对上述274份DNA标本的基因型分析，发现CD14-260基因型在正常对照组与UC组间无显著性差异.此基因多态性与UC无相关性.
结论:CD14基因C-260T多态性与中国湖北汉族UC无明显相关性.CD14基因不是溃疡性结肠炎的易感基因.
, 百拇医药
郭秋莎,夏冰. CD14基因C-260T多态性与溃疡性结肠炎的相关性.世界华人消化杂志 2005;13(12):1452-1454
Wright SD, Ramos RA, Tobias PS, Ulevitch RJ,Mathison JC. CD14, a receptor for complexes of lipopolysaccharide
(LPS) and LPS binding protein. Science 1990;249:1431-1433
2 da Silva CJ, Soldau K, Christen U, Tobias PS, Ulevitch RJ.Lipopolysaccharide is in close proximity to each of the proteins
, 百拇医药 in its membrane receptor complex. transferfrom CD14 to TLR4 and MD-2. J Biol Chem 2001;276:21129-21135
3 Pugin J, Heumann ID, Tomasz A, Kravchenko VV, Akamatsu Y, NishijimaM, Glauser MP, Tobias PS, Ulevitch RJ. CD14 is
a pattern recognition receptor. Immunity 1994;1:509-516
4 Schutt C. CD14. Int J Biochem Cell Biol 1999;31:545-549
5 Medzhitov R, Janeway C Jr. Innate immunity. N Engl J Med 2000;343:338-344
, 百拇医药
6 Frey EA, Miller DS, Jahr TG, Sundan A, Bazil V, Espevik T, FinlayBB, Wright SD. Soluble CD14 participates in the
response of cells to lipopolysaccharide. JExp Med 1992;176:1665-1671
7 Hubacek JA, Rothe G, Pit'haJ, Skodova Z, Stanek V, Poledne R, Schmitz G. C (-260) -->Tpolymorphism in the promoter
of the CD14 monocyte receptor gene as arisk factor for myocardial infarction. Circulation 1999;99:3218-3220
, 百拇医药
8 Baldini M, Lohman IC, Halonen M, Erickson RP, Holt PG, Martinez FD.A Polymorphism in the 5 flanking region
of the CD14 gene is associated withcirculating soluble CD14 levels and with total serum immunoglobulinE.
Am J Respir Cell Mol Biol 1999;20:976-983
9 Obana N, Takahashi S, Kinouchi Y, Negoro K, Takagi S, Hiwatashi N,Shimosegawa T. Ulcerative colitis is associated
, 百拇医药
with a promoter polymorphism oflipopolysaccharide receptor gene, CD14. Scand J Gastroenterol 2002;37:699-704
10 Klein W, Tromm A, Griga T, Fricke H, Folwaczny C, Hocke M, EitnerK, Marx M, Duerig N, Epplen JT. A polymorphism
in the CD14 gene is associated withCrohn disease. Scand J Gastroenterol 2002;37:189-191
11 Rioux JD, Silverberg MS, Daly MJ, Steinhart AH, McLeod RS,Griffiths AM, Green T, Brettin TS, Stone V, Bull SB,Bitton A, Williams CN, Greenberg GR,Cohen Z, Lander ES, Hudson TJ, Siminovitch KA. Genomewide search in
, 百拇医药
Canadian families with inflammatorybowel disease reveals two novel susceptibility loci. Am J Hum Genet
2000;66:1863-1870
12 中华医学会消化病学分会.对炎症性肠病诊断治疗规范的建议.中华消化杂志 2001;21:236-239
13 Simmons DL, Tan S, Tenen DG. Monocyte antigen CD14 is aphospholipid anchored membrane protein.
Blood 1989;73:284-289
14 Schutt C, Schilling T, Grunwald U, Schonfeld W, Kruger C. Endotoxin-neutralizingcapacity of soluble CD14.
, 百拇医药
Res Immunol 1992;143:71-78
15 Goyert SM, Ferrero E, Rettig WJ, Yenamandra AK, Obata F, Le BeauMM. The CD14 monocyte differentiation
antigen maps to aregion encoding growth factors and receptors. Science 1988;239:497-500
16 Zareie M, Singh PK, Irvine EJ, Sherman PM, McKay DM, Perdue MH.Monocyte/macrophage activation by normal
bacteria andbacterial products: implications for altered epithelial function inCrohn's disease. Am J Pathol
, 百拇医药
2001;158:1101-1109
17 Grimm MC, Pavli P, Van de PE, Doe WF. Evidence for a CD14+population of monocytes in inflammatory bowel
disease mucosa--implicationsfor pathogenesis. Clin Exp Immunol 1995;100:291-297
18 Rugtveit J, Haraldsen G, Hogasen AK, Bakka A, Brandtzaeg P, ScottH. Respiratory burst of intestinal macrophages
in inflammatorybowel disease is mainly caused by CD14+L1+ monocyte derived cells. Gut 1995;37:367-373
19 Ma Y, Ohmen JD, Li Z, Bentley LG, McElree C, Pressman S, Targan SR,Fischel-Ghodsian N, Rotter JI, Yang H. A
genome-wide searchidentifies potential new susceptibility loci for Crohn's disease. InflammBowel Dis 1999;5:271-278
编辑 王谨晖 审读 张海宁, 百拇医药( 郭秋莎, 夏 冰)
国家自然科学基金资助课题,No. 30370638
湖北省卫生厅重点项目(2003)
通讯作者:夏冰,430071, 湖北省武汉市东湖路169号,武汉大学中南医院综合医疗科，武汉大学中南医院消化病研究中心. bingxia2004@yahoo.com.cn
电话: 027-67812985 传真:027-87330795
收稿日期:2005-04-04 接受日期:2005-04-09
, http://www.100md.com
摘要目的:研究我国湖北汉族人CD14基因C-260T多态性的分布及CD14基因C-260T位点的突变是否与溃疡性结肠炎(UC)相关.
方法:采用PCR-RFLP方法检测114例UC患者以及160例正常对照者CD14-260基因型及等位基因频率.
结果:对上述274份DNA标本的基因型分析，发现CD14-260基因型在正常对照组与UC组间无显著性差异.此基因多态性与UC无相关性.
结论:CD14基因C-260T多态性与中国湖北汉族UC无明显相关性.CD14基因不是溃疡性结肠炎的易感基因.
, 百拇医药
郭秋莎,夏冰. CD14基因C-260T多态性与溃疡性结肠炎的相关性.世界华人消化杂志 2005;13(12):1452-1454
Wright SD, Ramos RA, Tobias PS, Ulevitch RJ,Mathison JC. CD14, a receptor for complexes of lipopolysaccharide
(LPS) and LPS binding protein. Science 1990;249:1431-1433
2 da Silva CJ, Soldau K, Christen U, Tobias PS, Ulevitch RJ.Lipopolysaccharide is in close proximity to each of the proteins
, 百拇医药 in its membrane receptor complex. transferfrom CD14 to TLR4 and MD-2. J Biol Chem 2001;276:21129-21135
3 Pugin J, Heumann ID, Tomasz A, Kravchenko VV, Akamatsu Y, NishijimaM, Glauser MP, Tobias PS, Ulevitch RJ. CD14 is
a pattern recognition receptor. Immunity 1994;1:509-516
4 Schutt C. CD14. Int J Biochem Cell Biol 1999;31:545-549
5 Medzhitov R, Janeway C Jr. Innate immunity. N Engl J Med 2000;343:338-344
, 百拇医药
6 Frey EA, Miller DS, Jahr TG, Sundan A, Bazil V, Espevik T, FinlayBB, Wright SD. Soluble CD14 participates in the
response of cells to lipopolysaccharide. JExp Med 1992;176:1665-1671
7 Hubacek JA, Rothe G, Pit'haJ, Skodova Z, Stanek V, Poledne R, Schmitz G. C (-260) -->Tpolymorphism in the promoter
of the CD14 monocyte receptor gene as arisk factor for myocardial infarction. Circulation 1999;99:3218-3220
, 百拇医药
8 Baldini M, Lohman IC, Halonen M, Erickson RP, Holt PG, Martinez FD.A Polymorphism in the 5 flanking region
of the CD14 gene is associated withcirculating soluble CD14 levels and with total serum immunoglobulinE.
Am J Respir Cell Mol Biol 1999;20:976-983
9 Obana N, Takahashi S, Kinouchi Y, Negoro K, Takagi S, Hiwatashi N,Shimosegawa T. Ulcerative colitis is associated
, 百拇医药
with a promoter polymorphism oflipopolysaccharide receptor gene, CD14. Scand J Gastroenterol 2002;37:699-704
10 Klein W, Tromm A, Griga T, Fricke H, Folwaczny C, Hocke M, EitnerK, Marx M, Duerig N, Epplen JT. A polymorphism
in the CD14 gene is associated withCrohn disease. Scand J Gastroenterol 2002;37:189-191
11 Rioux JD, Silverberg MS, Daly MJ, Steinhart AH, McLeod RS,Griffiths AM, Green T, Brettin TS, Stone V, Bull SB,Bitton A, Williams CN, Greenberg GR,Cohen Z, Lander ES, Hudson TJ, Siminovitch KA. Genomewide search in
, 百拇医药
Canadian families with inflammatorybowel disease reveals two novel susceptibility loci. Am J Hum Genet
2000;66:1863-1870
12 中华医学会消化病学分会.对炎症性肠病诊断治疗规范的建议.中华消化杂志 2001;21:236-239
13 Simmons DL, Tan S, Tenen DG. Monocyte antigen CD14 is aphospholipid anchored membrane protein.
Blood 1989;73:284-289
14 Schutt C, Schilling T, Grunwald U, Schonfeld W, Kruger C. Endotoxin-neutralizingcapacity of soluble CD14.
, 百拇医药
Res Immunol 1992;143:71-78
15 Goyert SM, Ferrero E, Rettig WJ, Yenamandra AK, Obata F, Le BeauMM. The CD14 monocyte differentiation
antigen maps to aregion encoding growth factors and receptors. Science 1988;239:497-500
16 Zareie M, Singh PK, Irvine EJ, Sherman PM, McKay DM, Perdue MH.Monocyte/macrophage activation by normal
bacteria andbacterial products: implications for altered epithelial function inCrohn's disease. Am J Pathol
, 百拇医药
2001;158:1101-1109
17 Grimm MC, Pavli P, Van de PE, Doe WF. Evidence for a CD14+population of monocytes in inflammatory bowel
disease mucosa--implicationsfor pathogenesis. Clin Exp Immunol 1995;100:291-297
18 Rugtveit J, Haraldsen G, Hogasen AK, Bakka A, Brandtzaeg P, ScottH. Respiratory burst of intestinal macrophages
in inflammatorybowel disease is mainly caused by CD14+L1+ monocyte derived cells. Gut 1995;37:367-373
19 Ma Y, Ohmen JD, Li Z, Bentley LG, McElree C, Pressman S, Targan SR,Fischel-Ghodsian N, Rotter JI, Yang H. A
genome-wide searchidentifies potential new susceptibility loci for Crohn's disease. InflammBowel Dis 1999;5:271-278
编辑 王谨晖 审读 张海宁, 百拇医药( 郭秋莎, 夏 冰)
