鞠砚 亢建民 臧培卓 邱吉庆 赵英志 丛福臣 刘春璞 李连发 亢建民(天津市环湖脑系科医院神经外科，天津 300060)；鞠砚 邱吉庆(白求恩医科大学第一临床学院神经外科，吉林 长春 130021)；臧培卓(白求恩医大一院神经外科博士研究生，吉林 长春 130021)；赵英志 丛福臣 刘春璞 李连发(白求恩医大一院神经外科进修生，吉林 长春 130021) 中风与神经疾病杂志 2000 0 17 4


    关键词：胶质瘤；凋亡；淋巴细胞；细胞因子 期刊 zfysjjbzz 0 224-226 论著与经验总结 fur -->


    

摘 要： 目的 细胞因子TNF、IFN等在淋巴细胞介导胶质瘤细胞凋亡方面的作用和特点。方法 采用免疫组化、分子生物学、MTT法及PBL对培养的胶质瘤细胞凋亡形成的直接观察方法。结果 正常人PBL对U-251MG的杀伤活性明显高于TNF或IFN的单独作用，IFN+PBL组对U-251MG的杀伤作用较PBL及TNF+PBL组的杀伤作用凋亡细胞很少而核分裂相细胞多；PBL+IFN组出现大量肿瘤细胞凋亡。结论 细胞凋亡调节失常是慢性增殖性肿瘤形成并发展的主要原因。从免疫活性细胞可以通过促凋亡方式抗肿瘤以来，如何通过调节免疫系统预防和治疗肿瘤已成为目前研究的热点。认为MTT法检测PBL介导胶质瘤细胞凋亡有特殊意义。在淋巴细胞抗肿瘤过程中介导释放IFN使肿瘤细胞TNFR及FasR表达增强，同时通过胞浆中的穿孔素，颗粒酶的释放导致肿瘤细胞凋亡。TNF引起的肿瘤杀伤作用是与TNFR相关的FasR介导的。FasL及抗Fas抗体与Fas集合后导致细胞凋亡。

    中图分类号： 739.41 文献标识码： A 文章编号： 1003-2754(2000)04-0224-03

The effect of cellular factors on glioma cell apoptosis induced by lymphocyte

JU Yan KANG Jian-min ZHANG Pei-zhuo et al.

    ( Department ofNeurosurgery,The First Teaching Hospital of Norman Bethun University of MedicalSciences,Changchun,130021,China )

Abstracts: Objective To investigate the effect ofcellular factors on glioma cell apoptosis induced by lymphocyte. Methods Usingimmunohistochemical,moleculobiological;MTT and direct observating methods to study combinecultured glioma cells;lymphocyte and cellular factors in first 4 hour. Results The killing activity of normalhuman PBL to U₂₅₁ MG is much higher than TNF or IFN. IFN plus PBL group is thebest one,the effect is better than PBL and PBL plus TNF,difference striking(P<0.05).Contral group has rare apoptosis cells but tumor cell apoptosis. Conclusion Lymphocyte release IFN whichincrease the tumor cells TNFR and FasR expretion,at the same time,perforin and granulinrelease from plasma and make tumor apoptosis. TNF induce tumor cell wounded is related toTNF and FasR. FasL and antifasantibody connected with Fas and lead to tumor cellapoptosis. It is very significante that using MTT method to research glioma cell apoptosisinduced by PBL.

     Keywords: Glioma; Apoptosis ； Lymphocyte ； Cellular factors. ▲

由于胶质瘤是一种难以根治的肿瘤 ......

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