毛 琳 申元英 李向群 李宗山 杨占秋 邱雨石 湖北医科大学病毒所临床病毒室，武汉430071 基础医学与临床 1999 0 19 6


    关键词：肾综合征出血热； 逆转录-聚合酶链； 酶联免疫吸咐法； 早期诊断 期刊 jcyxylc 0 研究论文 fur -->


    

摘要 为了选择更为敏感，特异，快速的肾综合征出血热(HFRS)的诊断方法。我们建立了碘化钠—异硫氰酸胍—氯仿法提取汉坦病毒(HV)核酸(RNA)用于逆转录一聚合酶链反应(RT-PCR)检测不同病型HFRS患者一周内血清中HV-RNA且与酶联免疫吸附法(ELISA)检测HFRS-IgM进行比较。45例HFRS患者血清中HV-RNA阳性率为82.2%，其中轻、中、重型阳性率分别为75%，78.5%，86.9%。HFRS-IgM阳性率55.5%，其中轻、中、重型阳性率分别为37.5%，50.0%， 65.2%。RT-PCR法检出率高于ELISA法，差别有显著性。RT-PCR法和ELISA法均可以用于HFRS的早期诊断。但前者更为敏感。

Early Diagnosis forHernorrhagle Fever with Rend Syndrome by Reverse-Transcription Polymerase Chain Reaction

Mao Ling SherlYuanying Li Xiangqur Let al

    (Vims Research Institute Hubei Medical University,Wuhan430071)

Abstract Selecting a more sensitive,specincearly diagnosis for hernorrhagic fever with renaisyndrome(HFRSL). A reverse-transcriptionpolymerase chain reaction(RT-PCR)detecting the hantavirus(HV)RNA in the sera Ofdifferent.types HFRS patients with Nal-guanidiym-thioeyanate-chloroform method extractingHV RNA was established and ELISA detecting the lgM was also performed for conparision.Thetotal pasitive rate of 45 HFRS cases ahd different type (mile,mediun,serious )patient'spositive rates are 82.2%,(75%,78%,86.9%)respectively by RT-PCR and55.5%(37.5%,50%,65.2%)respectively by EUSA.The detecting rates by RT-PCR aresignmeantlyhigher than those by ELISA.conclusions:Results suggested that both RT-PCR andELISA couldbe used for early diagnosis ofHFRS,and the RT.PCR is more sensitive than EUSA.

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