唐其东 林曙光 王若洁 唐其东(广东省心血管病研究所 510080)；林曙光(广东省心血管病研究所 510080)；王若洁(广东省心血管病研究所 510080) 中华心血管病杂志 2000 0 28 1


    关键词：反义寡核苷酸；C-sis原癌基因；内膜增生；局部干预 期刊 zhxxgbzz 0 69??71 基础研究 fur -->


    

摘 要：目的 研究主动脉球囊损伤后，C-sis原癌基因反义寡核苷酸(AODN)局部干预对血管内膜增生的影响。方法 36只新西兰白兔随机分为C-sis原癌基因AODN组、正义寡核苷酸(SODN)组及生理盐水组，每组12只。球囊损伤胸主动脉后，1mg AODN、SODN用于损伤局部干预，高胆固醇饲养， 用RT-PCR技术及组织学方法结合图像分析，观察AODN对损伤血管C-sis表达及内膜增生的影响。结果 AODN明显抑制损伤血管C-sis表达，SODN无抑制作用；AODN组、SODN组及生理盐水组，内膜增生厚度分别为(8.54±1.59)μm、(24.59±2.14) μm、(25.15±2.31) μm，AODN组平均抑制(65.7±9.6)%的内膜增生。结论 C-sis原癌基因AODN局部干预明显抑制球囊损伤血管内膜增生，其抑制作用与抑制损伤血管C-sis原癌基因表达密切关联。

Localdelivery of C-sis proto-oncogene antisense oligodeoxynucleotides inhibits intimalhyperplasia in rabbit injured aorta

TANG Qidong LIN Shuguang WANG Ruojie.

    ( GuangdongCardiovascular Institute, Guangzhou 510080, China )

Abstract ： Objective To study the inhibitory effect oflocally delivered C-sis proto-oncogene antisense oligodeoxynucleotides (AODN) via ballooncatheter on intimal injury following balloon angioplasty in rabbit aorta. Methods Thirty six rabbits were dividedinto AODN and sense oligodeoxynucleotides (SODN) treated groups and control group randomly(n=12, each). Each of the rabbits was injured with channeled balloon catheter 1 mg ofC-sis proto-oncogene AODN or SODN was locally delivered on injured aorta with inflatedballoon at 0.5×10³ kPa for 8 minutes. The rabbits were fed with 2.0%cholesterol diet for 3 days or 28 days. Expression of C-sis proto-oncogene was determinedby using of RT- PCR. Morphological analysis of intimal hyperplasia was performed withhistologic staining and image analyzer. Result AODN inhibited C-sis proto-onconge expression in injuredaorta obviously. SODN showed no effect on c- sis mRNA. Thickness of intimal hyperplasiafollowing locally delivered AODN, SODN and 0.9% NaCI solution on injured aorta was 8.54±1.59,24.59±2.14 and 25.15±2.31 μ m, respectively. Inhibitory rate of intimal hyperplasia with locally deliveredAODN was (65.7±9.6)%. Conclusion Local delivery of C-sis proto-oncongeneAODN following balloon angioplasty on injured aorta inhibits intimal hyperplasia, and theinhibitory effect was associated with suppression of c- sis proto-oncongene expression onthe injured aorta.

     ......

您现在查看是摘要页，全文长 13623 字符。