杨科 黄凌燕 100700 北京军区总医院血液科 中华血液学杂志 1999 0 20 12


    关键词：原位杂交，荧光；染色体异常；白血病，淋巴细胞性，急性；DNA探针 期刊 zhxyxzz 0 专 论 fur -->


    

【摘要 】 目的 研究荧光原位杂交技术(FISH)在检测异常染色体中的应用价值及其意义。方法 用FISH方法和染色体G分带技术对比观察急性淋巴细胞白血病(ALL)患者染色体的变化。结果 对38例患者17,18和21号染色体进行G分带技术检查，结果分别有11,13和18例患者细胞染色体存在三倍体细胞。FISH检查结果，除上述病例发现三倍体细胞外，染色体G分带检查正常者中也有部分病例存在三倍体。结论 FISH检测异常染色体，方法简便、灵敏、可靠，与常规细胞遗传学方法比较，FISH方法不需要培养细胞，对分裂期和分裂间期细胞均可进行基因定位检测，尤其在有核细胞少等不适于进行细胞遗传学分析的情况下，FISH方法仍可得到有意义的数据。

Detection of aberrant chromosomesin acute lymphoblastic leukemia by fluorescence in situ hybridization

YANG Ke,HUANG Lingyan.

    Beijing Army General Hospital of PLA,Beijing 100700

【Abstract 】 Objective Toinvestigate the value of fluorescence in situ hybridization (FISH) in detecting aberrantchromosomes in acute lymphoblastic leukemias. Methods Conventionalbanding techniques and FISH were used to detect trisomy 17,18 and 21 in 38 patients withacute lymphoblastic leukemia.Lymphoid DNA probes specific for chromosomes no.17,18 and 21were used for FISH. Results Out of the 38 patients,18 had +17,22 +18and 23 +21.The number of trisomy cells ranged from 7% to 80%. Ten normal bone marrowsamples were hybridized with the chromosomes 17,18 and 21 probes.Conclusion FISHis more sensitive than conventional cytogenetic analysis and allows systematic study onlarge cohort of patients without the need of metaphase preparations.

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