张广森 赵红霞 张琳 张广森 赵红霞 张琳 410011 长沙，湖南医科大学附属第二医院分子血液病研究室 中华血液学杂志 1999 0 20 9


    关键词：高半胱氨酸；内皮，血管；血小板聚集；抗凝血酶Ⅲ；肝素辅助因子Ⅱ 期刊 zhxyxzz 0 论 著 fur -->


    

摘要 目的 揭示高半胱氨酸(Hcy)致血栓形成的机制。方法 ①免疫荧光分析血管内皮细胞凝血酶调节蛋白(TM)抗原分布；②发色基质法测定肝素辅助因子Ⅱ(HCⅡ)活性。结果 ①Hcy可下调血管内皮细胞表面TM的表达，且呈浓度依赖性；②Hcy对ADP和凝血酶诱导的血小板聚集均无影响;③在正常人血浆体系中,以肝素和硫酸皮肤素(DS)为激动剂, Hcy对血浆AT-Ⅲ和HCⅡ的抗凝血酶活性无明显影响,在纯化的AT-Ⅲ体系, Hcy对AT-Ⅲ的抗凝血酶作用也无影响。结论 Hcy的致血栓效应与其对内皮细胞TM表达调节有关，且独立于血小板聚集功能和肝素辅助因子活性变化。

Effects of homocysteine onhuman vascular endothelial cells, platelet aggregation and heparin cofactor activity

ZHANG Guangsen, ZHAO Hongxia, ZHANG Lin.

    The Second Affiliated Hospital,Hunan Medical University, Changsha 410011

Abstract Objectives To elucidatepartly the mechanism of homocysteine-associated thrombosis. Methods ①Thrombomodulin(TM ) antigen distribution on human vascular endothelial cell (EC)membrane was assayed byimmune-fluorescence histochemistry stain and flow cytometry; ②Plasma heparin cofactoractivity was measured by chromogenic method. Results Homocysteine (Hcy) significantlydown-regulated the expression of TM on EC, with a concentration-dependent manner; ②Hcydid not influence platelet aggregation induced by ADP and thrombin; ③Hcy exerted noeffects on the activities of antithrombin Ⅲ or heparin cofactor. Conclusion The effectof Hcy in developing thrombosis may be associated with an inhibitory action on EC TMexpression;which was independent of platelet aggregation or heparin cofactor activity.

     ......

您现在查看是摘要页，全文长 10046 字符。