摘要 目的： 初步分析白血病细胞中IRF-1基因表达水平的变化。方法： 采用逆转录-聚合酶链反应，银染分析检测24例白血病患者和3种白血病细胞系中IRF-1基因表达水平。结果： 3例白血病患者未检测到IRF-1基因表达，5例患者及HL-60、HIMeg细胞系IRF-1基因表达明显降低。结论： 部分白血病患者的发病机制可能与IRF-1基因表达缺失或下降有关；IRF-1基因表达水平的变化可能对部分白血病患者的疗效观察及预后有参考价值。

    Study on expression of IRF-1 gene in human leukemia with RT-PCR and the silver sequence techniques. Xia Fang,Yuan Youzhong,Zhang Weiping,et al.ChanghaiHospital,The Second Military Medical University,Shanghai 200433

     Abstract Objective： To explore preliminarily the changes ofIRF-1 gene expression in leukemias.Methods： The expression of IRF-1 genein 24 leukemia patients and 3 leukemic cell lines were studied with RT-PCR and the silversequence techniques.Results： No expression of IRF-1 gene in 3patients,and significantly decreased expressions in 5 patients and in HL-60 and HIMeg celllines were revealed.Conclusion： The mechanism of human leukemogenesismay be partly associated with no or low-expression of IRF-1 gene.

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