摘要 目的： 观察γ干扰素(IFN-γ)、白细胞介素(IL)-10、IL-12对转染B7-1白血病细胞B7-1基因表达及其诱导健康人外周血单个核细胞(PBMC)产生IFN-γ、IL-2的影响。方法： 用逆转录-聚合酶链反应(RT-PCR)和酶联免疫吸附反应(ELISA)方法检测IFN-γ、IL-2基因表达和蛋白质合成。结果： 发现IFN-γ、IL-12增强PBMC产生IL-2、IFN-γ，IL-10抑制其产生，同时发现IFN-γ、IL-12增强转染B7-1白血病细胞B7-1基因表达，IL-10抑制其表达。结论： 细胞因子IFN-γ、IL-10、IL-12影响转染B7-1白血病细胞诱导PBMC产生IFN-γ、IL-2，其可能是通过影响B7-1基因表达实现的。

    The effect of cytokines on the activation of T cellsinduced by B7-1-transfected leukemia cells Huang Guiqing, Li Chengrong, Yang Xiqiang. Children's Hospital, Chongqing University of MedicalSciences, Chongqing 400014

     Abstract Objective: To observe the effects of IL-12, IFN-γ, IL-10 on the B7-1 expression of B7-1 transfected leukemia cells(K562 and HL-60) and on the IL-2 and IFN-γ expressionand production in the peripheral blood mononuclear cells(PBMC) induced by B7-1 transfectedK562 and HL-60 cells.Methods: RT-PCRand ELISA techniques were used to assay the expression and production of IFN-γ and IL-2. Results: IFN-γ and IL-12 increased the expressions of B7-1, IL-2 and IFN-γ, but IL-10 inhibited all the expressions. Conclusion: IFN-γ,IL-10 and IL-12 affected the expression and production of IFN-γand IL-2 possibly by affecting the B7-1 expression.

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